Search results for the GEO ID: GSE7835  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM190175 | GPL570 |
|
No_DFO_control 1
|
Untreated U251 cells not stimulated with DFO, biological replicate 1
|
cultured U251 cells
refer to ATTC
|
Untreated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM190175
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190175/suppl/GSM190175.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190175/suppl/GSM190175.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190176 | GPL570 |
|
No_DFO_control 2
|
Untreated U251 cells not stimulated with DFO, biological replicate 3
|
cultured U251 cells
refer to ATTC
|
Untreated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM190176
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190176/suppl/GSM190176.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190176/suppl/GSM190176.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190177 | GPL570 |
|
No_DFO_control 3
|
Untreated U251 cells not stimulated with DFO, biological replicate 3
|
cultured U251 cells
refer to ATTC
|
Untreated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM190177
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190177/suppl/GSM190177.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190177/suppl/GSM190177.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190178 | GPL570 |
|
Control 1
|
Untreated U251 cells stimulated with DFO, biological replicate 1
|
cultured U251 cells
refer to ATTC
|
Untreated LNCaP cells stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM190178
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190178/suppl/GSM190178.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190178/suppl/GSM190178.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190179 | GPL570 |
|
Control 2
|
Untreated U251 cells stimulated with DFO, biological replicate 2
|
cultured U251 cells
refer to ATTC
|
Untreated LNCaP cells stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM190179
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190179/suppl/GSM190179.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190179/suppl/GSM190179.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190180 | GPL570 |
|
Control 3
|
Untreated U251 cells stimulated with DFO, biological replicate 3
|
cultured U251 cells
refer to ATTC
|
Untreated LNCaP cells stimulated with DHT, biological replicate 3
|
| Sample_geo_accession | GSM190180
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190180/suppl/GSM190180.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190180/suppl/GSM190180.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190181 | GPL570 |
|
siRNA 1
|
siRNA treated U251 cells stimulated with DFO, biological replicate 1
|
cultured U251 cells
refer to ATTC
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM190181
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190181/suppl/GSM190181.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190181/suppl/GSM190181.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190182 | GPL570 |
|
siRNA 2
|
siRNA treated U251 cells stimulated with DFO, biological replicate 2
|
cultured U251 cells
refer to ATTC
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM190182
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190182/suppl/GSM190182.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190182/suppl/GSM190182.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190183 | GPL570 |
|
siRNA 3
|
siRNA treated U251 cells stimulated with DFO, biological replicate 3
|
cultured U251 cells
refer to ATTC
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 3
|
| Sample_geo_accession | GSM190183
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190183/suppl/GSM190183.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190183/suppl/GSM190183.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190184 | GPL570 |
|
Polyamide 1
|
Polyamide treated U251 cells stimulated with DFO, biological replicate 1
|
cultured U251 cells
refer to ATTC
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM190184
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190184/suppl/GSM190184.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190184/suppl/GSM190184.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190185 | GPL570 |
|
Polyamide 2
|
Polyamide treated U251 cells stimulated with DFO, biological replicate 2
|
cultured U251 cells
refer to ATTC
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM190185
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190185/suppl/GSM190185.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190185/suppl/GSM190185.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190186 | GPL570 |
|
Polyamide 3
|
Polyamide treated U251 cells stimulated with DFO, biological replicate 3
|
cultured U251 cells
refer to ATTC
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 3
|
| Sample_geo_accession | GSM190186
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190186/suppl/GSM190186.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190186/suppl/GSM190186.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190187 | GPL570 |
|
Ech 1
|
Echinomycin treated U251 cells stimulated with DFO, biological replicate 1
|
cultured U251 cells
refer to ATTC
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM190187
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190187/suppl/GSM190187.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190187/suppl/GSM190187.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190188 | GPL570 |
|
Ech 2
|
Echinomycin treated U251 cells stimulated with DFO, biological replicate 2
|
cultured U251 cells
refer to ATTC
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM190188
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190188/suppl/GSM190188.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190188/suppl/GSM190188.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
GSM190189 | GPL570 |
|
Ech 3
|
Echinomycin treated U251 cells stimulated with DFO, biological replicate 3
|
cultured U251 cells
refer to ATTC
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 3
|
| Sample_geo_accession | GSM190189
| | Sample_status | Public on May 17 2007
| | Sample_submission_date | May 17 2007
| | Sample_last_update_date | May 17 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
| | Sample_growth_protocol_ch1 | U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190189/suppl/GSM190189.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190189/suppl/GSM190189.CHP.gz
| | Sample_series_id | GSE7835
| | Sample_data_row_count | 54675
| | |
|
| |
| |
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(2 groups will be compared at a time) |
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