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Search results for the GEO ID: GSE7862
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GSM190617
GPL201
Normal buccal keratinocytes (NBK), Sample A Buccal mucosa from healthy, non-smoking donor undergoing maxillofacial surgery cell type: Normal oral keratinocytes in serum-free culture experimental factor: Un-exposed control Primary cell cultures were derived from tissue digestion with trypsin overnight at 4°C and the mixture was resuspended in a serum-free epithelial medium with high levels of amino acids (EMHA) and plated on dishes pre-coated with fibronectin and collagen.(Grafström R.C. Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). Cultures were transferred on regular tissue culture plastic at about 75 % confluence, and cells in passage 2 were used in the experiments.Total RNA was prepared from 3 x 10^6 cells using the RNeasy Mini Protocol (Qiagen GmbH, Hilden, Germany) from two separate experiments of NBK
GSM190618
GPL201
Normal buccal keratinocytes (NBK), sample B Buccal mucosa from healthy, non-smoking donor undergoing maxillofacial surgery cell type: Normal oral keratinocytes in serum-free culture experimental factor: Un-exposed control Primary cell cultures were derived from tissue digestion with trypsin overnight at 4°C and the mixture was resuspended in a serum-free epithelial medium with high levels of amino acids (EMHA) and plated on dishes pre-coated with fibronectin and collagen.(Grafström R.C. Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). Cultures were transferred on regular tissue culture plastic at about 75 % confluence, and cells in passage 2 were used in the experiments.Total RNA was prepared from 3 x 10^6 cells using the RNeasy Mini Protocol (Qiagen GmbH, Hilden, Germany) from two separate experiments of NBK.
GSM190619
GPL201
SV40 T-antigen transfected buccal keratinocyte line (SVpgC2a), Sample A Derived by transfection of SV40 T-antigen to human buccal keratinocytes cell line: The SVpgC2a cell line is cultured in the same serum-free epithelial medium with high levels of amino acids (EMHA) as normal buccal keratinocytes (Kulkarni et al. Carcinogenesis, 16: 2515-2521, 1995 and Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). experimental factor: Un-exposed control The SVpgC2a cell line is cultured in the same serum-free epithelial medium with high levels of amino acids (EMHA) as normal buccal keratinocytes (Kulkarni et al. Carcinogenesis, 16: 2515-2521, 1995 and Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). Total RNA was prepared from 3 x 10^6 cells using the RNeasy Mini Protocol (Qiagen GmbH, Hilden, Germany) from two separate experiments of SVpgC2a in passages 64-72.
GSM190620
GPL201
SV40 T-antigen transfected buccal keratinocyte line (SVpgC2a), Sample B Derived by transfection of SV40 T-antigen to human buccal keratinocytes cell line: The SVpgC2a cell line is cultured in the same serum-free epithelial medium with high levels of amino acids (EMHA) as normal buccal keratinocytes (Kulkarni et al. Carcinogenesis, 16: 2515-2521, 1995 and Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). experimental factor: Un-exposed control The SVpgC2a cell line is cultured in the same serum-free epithelial medium with high levels of amino acids (EMHA) as normal buccal keratinocytes (Kulkarni et al. Carcinogenesis, 16: 2515-2521, 1995 and Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). Total RNA was prepared from 3 x 10^6 cells using the RNeasy Mini Protocol (Qiagen GmbH, Hilden, Germany) from two separate experiments of SVpgC2a in passages 64-72.
GSM190621
GPL201
Buccal carcinoma cell line (SqCC/Y1), Sample A Buccal carcinoma cell line: The SqCC/Y1 cells are cultured in the same serum-free epithelial medium with high levels of amino acids (EMHA) as normal buccal keratinocytes (Sundkvist, K et al. Cancer Commun, 3: 331-340, 1991 and Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). experimental factor: Un-exposed control The SqCC/Y1 cells are cultured in the same serum-free epithelial medium with high levels of amino acids (EMHA) as normal buccal keratinocytes (Sundkvist, K et al. Cancer Commun, 3: 331-340, 1991 and Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). Total RNA was prepared from 3 x 10^6 cells using the RNeasy Mini Protocol (Qiagen GmbH, Hilden, Germany) from two separate experiments of SqCC/Y1 in passages 125-129.
GSM190622
GPL201
Buccal carcinoma cell line (SqCC/Y1), Sample B Buccal carcinoma cell line: The SqCC/Y1 cells are cultured in the same serum-free epithelial medium with high levels of amino acids (EMHA) as normal buccal keratinocytes (Sundkvist, K et al. Cancer Commun, 3: 331-340, 1991 and Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). experimental factor: Un-exposed control The SqCC/Y1 cells are cultured in the same serum-free epithelial medium with high levels of amino acids (EMHA) as normal buccal keratinocytes (Sundkvist, K et al. Cancer Commun, 3: 331-340, 1991 and Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). Total RNA was prepared from 3 x 10^6 cells using the RNeasy Mini Protocol (Qiagen GmbH, Hilden, Germany) from two separate experiments of SqCC/Y1 in passages 125-129
 
 
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