Search results for the GEO ID: GSE7874  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM190964 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A1P1d07EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
21-50 age
M/F
Day 7: proerythroblast
|
| Sample_geo_accession | GSM190964
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190964/suppl/GSM190964.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190965 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A2P2d07EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
26-37 age
M/F
Day 7: proerythroblast
|
| Sample_geo_accession | GSM190965
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190965/suppl/GSM190965.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190966 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A3P3d07EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
25-45 age
M/F
Day 7: proerythroblast
|
| Sample_geo_accession | GSM190966
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190966/suppl/GSM190966.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190967 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A4P4d07EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
22-42 age
M/F
Day 7: proerythroblast
|
| Sample_geo_accession | GSM190967
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190967/suppl/GSM190967.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190968 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A5P5d07EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
20-30 age
M/F
Day 7: proerythroblast
|
| Sample_geo_accession | GSM190968
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190968/suppl/GSM190968.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190969 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A16P1d14EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 14
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: mature erythrocytes
tissue type: primary cells from donated blood
|
Pool of 3 samples:
21-50 age
M/F
Day 14: mature erythrocytes
|
| Sample_geo_accession | GSM190969
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190969/suppl/GSM190969.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190970 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A17P2d14EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 14
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: mature erythrocytes
tissue type: primary cells from donated blood
|
Pool of 3 samples:
26-37 age
M/F
Day 14: mature erythrocytes
|
| Sample_geo_accession | GSM190970
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190970/suppl/GSM190970.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190971 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A18P3d14EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 14
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: mature erythrocytes
tissue type: primary cells from donated blood
|
Pool of 3 samples:
25-45 age
M/F
Day 14: mature erythrocytes
|
| Sample_geo_accession | GSM190971
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190971/suppl/GSM190971.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190972 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A19P4d14EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 14
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: mature erythrocytes
tissue type: primary cells from donated blood
|
Pool of 3 samples:
22-42 age
M/F
Day 14: mature erythrocytes
|
| Sample_geo_accession | GSM190972
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190972/suppl/GSM190972.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM190973 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A20P5d14EPO
|
CD34+ progenitor cells subjected to EPO and harvested at day 14
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: mature erythrocytes
tissue type: primary cells from donated blood
|
Pool of 3 samples:
20-30 age
M/F
Day 14: mature erythrocytes
|
| Sample_geo_accession | GSM190973
| | Sample_status | Public on Aug 22 2007
| | Sample_submission_date | May 22 2007
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EPO (4U/ml)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. Trizol LS and Qiagen Rneasy Mini column clean up
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM190nnn/GSM190973/suppl/GSM190973.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM426506 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A6P1d07EST
|
CD34+ cell in EST condition on day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
Donor: CD092404/CD071604/CD040403
Age: 37/21/50
Sex: M/F/F
Day: d7/d7/d7
|
| Sample_geo_accession | GSM426506
| | Sample_status | Public on Jul 10 2009
| | Sample_submission_date | Jul 10 2009
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EST [Erythropoietin (EPO) =4U/ml, Stem Cell Factor (SCF)=50ng/ml, and Transforming growth factor beta-1 (TGFbeta-1)=1.25ng/ml]
| | Sample_growth_protocol_ch1 | After obtaining informed consent, human CD34+ cells were isolated in high purity from the peripheral blood of normal human volunteers. The cells were cultured at a concentration of 104 -105 cells/mL in medium supplemented with 4 U/mL EPO (Amgen, Thousand Oaks, CA), 50ng/ml SCF, and 1.25ng/ml TGFbeta1 (R&D systems) as described previously (PMID: 15367428). Cells were enumerated using an electronic cell counter (Coulter, Hialeah, FL). Adult and fetal hemoglobin was analysed by HPLC.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. RNA was extracted using QIAshredder and Rneasy Minikit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM426nnn/GSM426506/suppl/GSM426506.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM426507 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A7P2d07EST
|
CD34+ cell in EST condition on day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
Donor: CD043004/CD021304/CD032103
Age: 33/37/26
Sex: F/F/M
Day: d7/d7/d7
|
| Sample_geo_accession | GSM426507
| | Sample_status | Public on Jul 10 2009
| | Sample_submission_date | Jul 10 2009
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EST [Erythropoietin (EPO) =4U/ml, Stem Cell Factor (SCF)=50ng/ml, and Transforming growth factor beta-1 (TGFbeta-1)=1.25ng/ml]
| | Sample_growth_protocol_ch1 | After obtaining informed consent, human CD34+ cells were isolated in high purity from the peripheral blood of normal human volunteers. The cells were cultured at a concentration of 104 -105 cells/mL in medium supplemented with 4 U/mL EPO (Amgen, Thousand Oaks, CA), 50ng/ml SCF, and 1.25ng/ml TGFbeta1 (R&D systems) as described previously (PMID: 15367428). Cells were enumerated using an electronic cell counter (Coulter, Hialeah, FL). Adult and fetal hemoglobin was analysed by HPLC.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. RNA was extracted using QIAshredder and Rneasy Minikit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM426nnn/GSM426507/suppl/GSM426507.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM426508 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A8P3d07EST
|
CD34+ cell in EST condition on day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
Donor: CD073004/CD062102/CD062802
Age: 25/45/31
Sex: M/M/F
Day: d7/d7/d7
|
| Sample_geo_accession | GSM426508
| | Sample_status | Public on Jul 10 2009
| | Sample_submission_date | Jul 10 2009
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EST [Erythropoietin (EPO) =4U/ml, Stem Cell Factor (SCF)=50ng/ml, and Transforming growth factor beta-1 (TGFbeta-1)=1.25ng/ml]
| | Sample_growth_protocol_ch1 | After obtaining informed consent, human CD34+ cells were isolated in high purity from the peripheral blood of normal human volunteers. The cells were cultured at a concentration of 104 -105 cells/mL in medium supplemented with 4 U/mL EPO (Amgen, Thousand Oaks, CA), 50ng/ml SCF, and 1.25ng/ml TGFbeta1 (R&D systems) as described previously (PMID: 15367428). Cells were enumerated using an electronic cell counter (Coulter, Hialeah, FL). Adult and fetal hemoglobin was analysed by HPLC.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. RNA was extracted using QIAshredder and Rneasy Minikit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM426nnn/GSM426508/suppl/GSM426508.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM426509 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A9P4d07EST
|
CD34+ cell in EST condition on day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
Donor: CD052804/CD013004/CD050903
Age: 23/42/22
Sex: M/F/F
Day: d7/d7/d7
|
| Sample_geo_accession | GSM426509
| | Sample_status | Public on Jul 10 2009
| | Sample_submission_date | Jul 10 2009
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EST [Erythropoietin (EPO) =4U/ml, Stem Cell Factor (SCF)=50ng/ml, and Transforming growth factor beta-1 (TGFbeta-1)=1.25ng/ml]
| | Sample_growth_protocol_ch1 | After obtaining informed consent, human CD34+ cells were isolated in high purity from the peripheral blood of normal human volunteers. The cells were cultured at a concentration of 104 -105 cells/mL in medium supplemented with 4 U/mL EPO (Amgen, Thousand Oaks, CA), 50ng/ml SCF, and 1.25ng/ml TGFbeta1 (R&D systems) as described previously (PMID: 15367428). Cells were enumerated using an electronic cell counter (Coulter, Hialeah, FL). Adult and fetal hemoglobin was analysed by HPLC.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. RNA was extracted using QIAshredder and Rneasy Minikit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM426nnn/GSM426509/suppl/GSM426509.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
GSM426510 | GPL570 |
|
06-14-05_Miller_U133Plus2_0_A10P5d07EST
|
CD34+ cell in EST condition on day 7
|
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
Pool of 3 samples:
Donor: CD072304/CD062504/CD060702
Age: 20/30/23
Sex: M/F/F
Day: d7/d7/d7
|
| Sample_geo_accession | GSM426510
| | Sample_status | Public on Jul 10 2009
| | Sample_submission_date | Jul 10 2009
| | Sample_last_update_date | Jul 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | EST [Erythropoietin (EPO) =4U/ml, Stem Cell Factor (SCF)=50ng/ml, and Transforming growth factor beta-1 (TGFbeta-1)=1.25ng/ml]
| | Sample_growth_protocol_ch1 | After obtaining informed consent, human CD34+ cells were isolated in high purity from the peripheral blood of normal human volunteers. The cells were cultured at a concentration of 104 -105 cells/mL in medium supplemented with 4 U/mL EPO (Amgen, Thousand Oaks, CA), 50ng/ml SCF, and 1.25ng/ml TGFbeta1 (R&D systems) as described previously (PMID: 15367428). Cells were enumerated using an electronic cell counter (Coulter, Hialeah, FL). Adult and fetal hemoglobin was analysed by HPLC.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. RNA was extracted using QIAshredder and Rneasy Minikit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | standard affymetrix protocol
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffery,Lynn,Miller
| | Sample_contact_email | jm7f@nih.gov
| | Sample_contact_phone | 301-480-1908
| | Sample_contact_fax | 301-435-5148
| | Sample_contact_laboratory | Molecular Biology and Genetics Section
| | Sample_contact_department | Molecular Medicine Branch
| | Sample_contact_institute | NIH/NIDDK
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM426nnn/GSM426510/suppl/GSM426510.CEL.gz
| | Sample_series_id | GSE7874
| | Sample_data_row_count | 54675
| | |
|
| |
| |
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