Search results for the GEO ID: GSE7888 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM194075 | GPL570 |
|
mesenchymal stem cells, lot#4F1127, 4th passage
|
mesenchymal stem cells, lot#4F1127, 4th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F1127, passage number 4
|
Sample_geo_accession | GSM194075
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194075/suppl/GSM194075.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194076 | GPL570 |
|
mesenchymal stem cells, lot#4F0312, 4th passage
|
mesenchymal stem cells, lot#4F0312, 4th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0312, passage number 4
|
Sample_geo_accession | GSM194076
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194076/suppl/GSM194076.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194077 | GPL570 |
|
mesenchymal stem cells, lot#5F0138, 4th passage
|
mesenchymal stem cells, lot#5F0138, 4th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#5F0138, passage number 4
|
Sample_geo_accession | GSM194077
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194077/suppl/GSM194077.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194078 | GPL570 |
|
mesenchymal stem cells, lot#4F0591, 4th passage
|
mesenchymal stem cells, lot#4F0591, 4th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0591, passage number 4
|
Sample_geo_accession | GSM194078
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194078/suppl/GSM194078.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194079 | GPL570 |
|
mesenchymal stem cells, lot#4F0760, 4th passage
|
mesenchymal stem cells, lot#4F0760, 4th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0760, passage number 4
|
Sample_geo_accession | GSM194079
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194079/suppl/GSM194079.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194080 | GPL570 |
|
mesenchymal stem cells, lot#4F1560, 5th passage
|
mesenchymal stem cells, lot#4F1560, 5th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F1560, passage number 5
|
Sample_geo_accession | GSM194080
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194080/suppl/GSM194080.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194081 | GPL570 |
|
mesenchymal stem cells, lot#4F1127, 7th passage
|
mesenchymal stem cells, lot#4F1127, 7th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F1127, passage number 7
|
Sample_geo_accession | GSM194081
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194081/suppl/GSM194081.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194082 | GPL570 |
|
mesenchymal stem cells, lot#4F0312, 7th passage
|
mesenchymal stem cells, lot#4F0312, 7th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0312, passage number 7
|
Sample_geo_accession | GSM194082
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194082/suppl/GSM194082.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194083 | GPL570 |
|
mesenchymal stem cells, lot#5F0138, 7th passage
|
mesenchymal stem cells, lot#5F0138, 7th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#5F0138, passage number 7
|
Sample_geo_accession | GSM194083
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194083/suppl/GSM194083.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194084 | GPL570 |
|
mesenchymal stem cells, lot#4F1560, 7th passage
|
mesenchymal stem cells, lot#4F1560, 7th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F1560, passage number 7
|
Sample_geo_accession | GSM194084
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194084/suppl/GSM194084.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194085 | GPL570 |
|
mesenchymal stem cells, lot#4F0591, 7th passage
|
mesenchymal stem cells, lot#4F0591, 7th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0591, passage number 7
|
Sample_geo_accession | GSM194085
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194085/suppl/GSM194085.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194086 | GPL570 |
|
mesenchymal stem cells, lot#4F1127, 9th passage
|
mesenchymal stem cells, lot#4F1127, 9th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F1127, passage number 9
|
Sample_geo_accession | GSM194086
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194086/suppl/GSM194086.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194087 | GPL570 |
|
mesenchymal stem cells, lot#4F0312, 9th passage
|
mesenchymal stem cells, lot#4F0312, 9th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0312, passage number 9
|
Sample_geo_accession | GSM194087
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194087/suppl/GSM194087.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194088 | GPL570 |
|
mesenchymal stem cells, lot#5F0138, 9th passage
|
mesenchymal stem cells, lot#5F0138, 9th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#5F0138, passage number 9
|
Sample_geo_accession | GSM194088
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194088/suppl/GSM194088.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194089 | GPL570 |
|
mesenchymal stem cells, lot#4F1560, 9th passage
|
mesenchymal stem cells, lot#4F1560, 9th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F1560, passage number 9
|
Sample_geo_accession | GSM194089
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194089/suppl/GSM194089.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194090 | GPL570 |
|
mesenchymal stem cells, lot#4F0591, 9th passage
|
mesenchymal stem cells, lot#4F0591, 9th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0591, passage number 9
|
Sample_geo_accession | GSM194090
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194090/suppl/GSM194090.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194091 | GPL570 |
|
mesenchymal stem cells, lot#4F0760, 9th passage
|
mesenchymal stem cells, lot#4F0760, 9th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.2
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0760, passage number 9
|
Sample_geo_accession | GSM194091
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194091/suppl/GSM194091.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194092 | GPL570 |
|
mesenchymal stem cells, lot#4F1127, 22nd passage
|
mesenchymal stem cells, lot#4F1127, 22nd passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F1127, passage number 22
|
Sample_geo_accession | GSM194092
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194092/suppl/GSM194092.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194093 | GPL570 |
|
mesenchymal stem cells, lot#5F0138, 24th passage
|
mesenchymal stem cells, lot#5F0138, 24th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#5F0138, passage number 24
|
Sample_geo_accession | GSM194093
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194093/suppl/GSM194093.CEL.gz
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194094 | GPL570 |
|
mesenchymal stem cells, lot#4F0312, 28th passage
|
mesenchymal stem cells, lot#4F0312, 28th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0312, passage number 28
|
Sample_geo_accession | GSM194094
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194094/suppl/GSM194094.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194095 | GPL570 |
|
mesenchymal stem cells, lot#4F1560, 28th passage
|
mesenchymal stem cells, lot#4F1560, 28th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F1560, passage number 28
|
Sample_geo_accession | GSM194095
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194095/suppl/GSM194095.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
| |
|
GSM194096 | GPL570 |
|
mesenchymal stem cells, lot#4F0591, 28th passage
|
mesenchymal stem cells, lot#4F0591, 28th passage
|
bone marrow mesenchymal stem cells
|
GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0591, passage number 28
|
Sample_geo_accession | GSM194096
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194096/suppl/GSM194096.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
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GSM194097 | GPL570 |
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mesenchymal stem cells, lot#4F0760, 28th passage
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mesenchymal stem cells, lot#4F0760, 28th passage
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bone marrow mesenchymal stem cells
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GCOS1.4
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), lot#4F0760, passage number 28
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Sample_geo_accession | GSM194097
| Sample_status | Public on Jun 06 2008
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Jun 06 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle Affymetrix protocol from 100 ng (Two-cycle) of total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| Sample_platform_id | GPL570
| Sample_contact_name | Shihori,,Tanabe
| Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| Sample_contact_institute | National Institute of Health Sciences
| Sample_contact_address | 1-18-1, Kami-yoga,
| Sample_contact_city | Setagaya-ku
| Sample_contact_state | Tokyo
| Sample_contact_zip/postal_code | 158-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194097/suppl/GSM194097.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE7888
| Sample_data_row_count | 54675
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