Search results for the GEO ID: GSE7890 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM194104 | GPL570 |
|
keloid treated with hydrocortisone-1
|
Primary culture of dermal fibroblasts from keloid, treated with 1.5uM hydrocortisone
|
strain: 33
Disorder: keloid
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194104
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194104/suppl/GSM194104.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194105 | GPL570 |
|
keloid treated with hydrocortisone-2
|
Primary culture of dermal fibroblasts from keloid, treated with 1.5uM hydrocortisone
|
strain: 50
Disorder: keloid
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194105
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194105/suppl/GSM194105.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194106 | GPL570 |
|
keloid treated with hydrocortisone-3
|
Primary culture of dermal fibroblasts from keloid, treated with 1.5uM hydrocortisone
|
strain: 145
Disorder: keloid
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194106
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194106/suppl/GSM194106.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194107 | GPL570 |
|
keloid treated with hydrocortisone-4
|
Primary culture of dermal fibroblasts from keloid, treated with 1.5uM hydrocortisone
|
strain: 261
Disorder: keloid
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194107
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194107/suppl/GSM194107.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194108 | GPL570 |
|
keloid treated with hydrocortisone-5
|
Primary culture of dermal fibroblasts from keloid, treated with 1.5uM hydrocortisone
|
strain: 124
Disorder: keloid
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194108
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194108/suppl/GSM194108.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194109 | GPL570 |
|
keloid with no hydrocortisone treatment-1
|
Primary culture of dermal fibroblasts from keloid, no hydrocortisone treatment
|
strain: 145
Disorder: keloid
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194109
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194109/suppl/GSM194109.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194110 | GPL570 |
|
keloid with no hydrocortisone treatment-2
|
Primary culture of dermal fibroblasts from keloid, no hydrocortisone treatment
|
strain: 261
Disorder: keloid
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194110
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194110/suppl/GSM194110.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194111 | GPL570 |
|
keloid with no hydrocortisone treatment-3
|
Primary culture of dermal fibroblasts from keloid, no hydrocortisone treatment
|
strain: 124
Disorder: keloid
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194111
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194111/suppl/GSM194111.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194112 | GPL570 |
|
keloid with no hydrocortisone treatment-4
|
Primary culture of dermal fibroblasts from keloid, no hydrocortisone treatment
|
strain: 33
Disorder: keloid
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194112
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194112/suppl/GSM194112.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194113 | GPL570 |
|
keloid with no hydrocortisone treatment-5
|
Primary culture of dermal fibroblasts from keloid, no hydrocortisone treatment
|
strain: 50
Disorder: keloid
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194113
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194113/suppl/GSM194113.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194114 | GPL570 |
|
normal scar treated with hydrocortisone-1
|
Primary culture of dermal fibroblasts from normal scar, treated with 1.5uM hydrocortisone
|
strain: 116
Disorder: normal scar
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194114
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194114/suppl/GSM194114.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194115 | GPL570 |
|
normal scar treated with hydrocortisone-2
|
Primary culture of dermal fibroblasts from normal scar, treated with 1.5uM hydrocortisone
|
strain: 131
Disorder: normal scar
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194115
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194115/suppl/GSM194115.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194116 | GPL570 |
|
normal scar treated with hydrocortisone-3
|
Primary culture of dermal fibroblasts from normal scar, treated with 1.5uM hydrocortisone
|
strain: 170
Disorder: normal scar
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194116
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194116/suppl/GSM194116.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194117 | GPL570 |
|
normal scar treated with hydrocortisone-4
|
Primary culture of dermal fibroblasts from normal scar, treated with 1.5uM hydrocortisone
|
strain: 21
Disorder: normal scar
Condition: +hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194117
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194117/suppl/GSM194117.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194118 | GPL570 |
|
normal with no hydrocortisone treatment-1
|
Primary culture of dermal fibroblasts from normal scar, no hydrocortisone treatment
|
strain: 21
Disorder: normal
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194118
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194118/suppl/GSM194118.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194119 | GPL570 |
|
normal with no hydrocortisone treatment-2
|
Primary culture of dermal fibroblasts from normal scar, no hydrocortisone treatment
|
strain: 170
Disorder: normal
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194119
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194119/suppl/GSM194119.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194120 | GPL570 |
|
normal with no hydrocortisone treatment-3
|
Primary culture of dermal fibroblasts from normal scar, no hydrocortisone treatment
|
strain: 58
Disorder: normal
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194120
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194120/suppl/GSM194120.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194121 | GPL570 |
|
normal with no hydrocortisone treatment-4
|
Primary culture of dermal fibroblasts from normal scar, no hydrocortisone treatment
|
strain: 116
Disorder: normal
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194121
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194121/suppl/GSM194121.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
|
GSM194122 | GPL570 |
|
normal with no hydrocortisone treatment-5
|
Primary culture of dermal fibroblasts from normal scar, no hydrocortisone treatment
|
strain: 131
Disorder: normal
Condition: no hydrocortisone
|
Cell cultures were initiated from human biopsy material from normal dermal scars and keloids of adult males and females and frozen at first or second passage, in liquid nitrogen. Experimental cultures were derived from the first passage of cells thawed from liquid nitrogen.
|
Sample_geo_accession | GSM194122
| Sample_status | Public on Nov 26 2007
| Sample_submission_date | May 24 2007
| Sample_last_update_date | Nov 26 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_growth_protocol_ch1 | Five strains each of cultured low-passage fibroblasts from keloids and normal scars were thawed from liquid nitrogen, subcultured and triplicate cultures grown to confluency (10 days) under standard culture conditions (daily feeding with Ham’s Nutrient Mixture F10 + 10% FBS) with and without 1.5 micromolar hydrocortisone.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using a standard Trizol method with DNase treatment followed by a second Trizol extraction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Following quality control, the RNA was prepared for microarray analysis using the standard Affymetrix protocol (Affymetrix Inc, Santa Clara, CA). Briefly, a total of 5 µg of total RNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was then carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
| Sample_hyb_protocol | Hybridized cRNA was detected using streptavidin coupled to phycoerythrin and visualized using GeneChip Scanner 3000 7G.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G and GeneChip Operating System (GCOS, Affymetrix, Santa Clara, CA). Default values were used to grid images (.DAT) and generate .CEL and .CHP files and to generate gene expression values and ratios of gene expression between the hybridized samples
| Sample_data_processing | For statistical analysis, CEL files (raw Affymetrix data) were imported in GeneSpring 7.0 (Agilent Technologies) and transformed by RMA (Robust Multichip Analysis).
| Sample_platform_id | GPL570
| Sample_contact_name | Shirley,Brody,Russell
| Sample_contact_email | shirley.b.russell@vanderbilt.edu
| Sample_contact_phone | 615-343-5853
| Sample_contact_fax | 615-343-8619
| Sample_contact_department | Medicine
| Sample_contact_institute | Vanderbilt University
| Sample_contact_address | 519 Light Hall
| Sample_contact_city | Nashville
| Sample_contact_state | TN
| Sample_contact_zip/postal_code | 37232-0700
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM194nnn/GSM194122/suppl/GSM194122.CEL.gz
| Sample_series_id | GSE7890
| Sample_data_row_count | 54675
| |
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Select GSMs and click on "Add groups" |
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