Search results for the GEO ID: GSE7970 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM197268 | GPL1355 |
|
+/+ Bottom 1_Sample1
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Bottom 1_Sample1
|
Sample_geo_accession | GSM197268
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197268/suppl/GSM197268.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197269 | GPL1355 |
|
+/+ Bottom 2_Sample3
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Bottom 2_Sample3
|
Sample_geo_accession | GSM197269
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197269/suppl/GSM197269.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197270 | GPL1355 |
|
+/+ Bottom 3_Sample5
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Bottom 3_Sample5
|
Sample_geo_accession | GSM197270
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197270/suppl/GSM197270.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197271 | GPL1355 |
|
+/+ Bottom 4_Sample7
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Bottom 4_Sample7
|
Sample_geo_accession | GSM197271
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197271/suppl/GSM197271.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197272 | GPL1355 |
|
+/+ Top 1_Sample2
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Top 1_Sample2
|
Sample_geo_accession | GSM197272
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197272/suppl/GSM197272.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197273 | GPL1355 |
|
+/+ Top 2_Sample4
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Top 2_Sample4
|
Sample_geo_accession | GSM197273
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197273/suppl/GSM197273.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197274 | GPL1355 |
|
+/+ top 3_Sample6
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ top 3_Sample6
|
Sample_geo_accession | GSM197274
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197274/suppl/GSM197274.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197275 | GPL1355 |
|
+/+ Top 4_Sample8
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Top 4_Sample8
|
Sample_geo_accession | GSM197275
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197275/suppl/GSM197275.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197276 | GPL1355 |
|
Iron-def Bottom 1_Sample9
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Iron-def Bottom 1_Sample9
|
Sample_geo_accession | GSM197276
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197276/suppl/GSM197276.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197277 | GPL1355 |
|
Iron-def Bottom 2_Sample11
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Iron-def Bottom 2_Sample11
|
Sample_geo_accession | GSM197277
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197277/suppl/GSM197277.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197278 | GPL1355 |
|
Iron-def Bottom 3_Sample13
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Iron-def Bottom 3_Sample13
|
Sample_geo_accession | GSM197278
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197278/suppl/GSM197278.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197279 | GPL1355 |
|
Iron-def Bottom 4_Sample15
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Iron-def Bottom 4_Sample15
|
Sample_geo_accession | GSM197279
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197279/suppl/GSM197279.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197280 | GPL1355 |
|
Iron-def Top 1_Sample10
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Iron-def Top 1_Sample10
|
Sample_geo_accession | GSM197280
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197280/suppl/GSM197280.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197281 | GPL1355 |
|
Iron-def Top 2_Sample12
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Iron-def Top 2_Sample12
|
Sample_geo_accession | GSM197281
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197281/suppl/GSM197281.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197282 | GPL1355 |
|
Iron-def Top 3_Sample14
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Iron-def Top 3_Sample14
|
Sample_geo_accession | GSM197282
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197282/suppl/GSM197282.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197283 | GPL1355 |
|
Iron-def Top 4_Sample16
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Iron-def Top 4_Sample16
|
Sample_geo_accession | GSM197283
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197283/suppl/GSM197283.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197284 | GPL1355 |
|
Fe-repletion (3d) Bottom 1_Sample17
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Fe-repletion (3d) Bottom 1_Sample17
|
Sample_geo_accession | GSM197284
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197284/suppl/GSM197284.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197285 | GPL1355 |
|
Fe-repletion (3d) 2 Bottom_Sample19
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Fe-repletion (3d) 2 Bottom_Sample19
|
Sample_geo_accession | GSM197285
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197285/suppl/GSM197285.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197286 | GPL1355 |
|
Fe-repletion (3d) 3 Bottom_Sample21
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Fe-repletion (3d) 3 Bottom_Sample21
|
Sample_geo_accession | GSM197286
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197286/suppl/GSM197286.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197287 | GPL1355 |
|
Fe-repletion (3d) Top 1_Sample18
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Fe-repletion (3d) Top 1_Sample18
|
Sample_geo_accession | GSM197287
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197287/suppl/GSM197287.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197288 | GPL1355 |
|
Fe-repletion (3d) Top 2_Sample20
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Fe-repletion (3d) Top 2_Sample20
|
Sample_geo_accession | GSM197288
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197288/suppl/GSM197288.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197289 | GPL1355 |
|
Fe-repletion (3d) Top 3_Sample22
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
Fe-repletion (3d) Top 3_Sample22
|
Sample_geo_accession | GSM197289
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197289/suppl/GSM197289.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197290 | GPL1355 |
|
b/b Bottom 1_Sample23
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b Bottom 1_Sample23
|
Sample_geo_accession | GSM197290
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197290/suppl/GSM197290.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197291 | GPL1355 |
|
b/b Bottom 2_Sample25
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b Bottom 2_Sample25
|
Sample_geo_accession | GSM197291
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197291/suppl/GSM197291.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197292 | GPL1355 |
|
b/b Bottom 3_Sample27
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b Bottom 3_Sample27
|
Sample_geo_accession | GSM197292
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197292/suppl/GSM197292.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197293 | GPL1355 |
|
b/b Bottom 4_Sample29
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b Bottom 4_Sample29
|
Sample_geo_accession | GSM197293
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197293/suppl/GSM197293.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197294 | GPL1355 |
|
b/b Top 1_Sample24
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b Top 1_Sample24
|
Sample_geo_accession | GSM197294
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197294/suppl/GSM197294.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197295 | GPL1355 |
|
b/b Top 2_Sample26
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b Top 2_Sample26
|
Sample_geo_accession | GSM197295
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197295/suppl/GSM197295.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197296 | GPL1355 |
|
b/b Top 3_Sample28
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b Top 3_Sample28
|
Sample_geo_accession | GSM197296
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197296/suppl/GSM197296.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197297 | GPL1355 |
|
b/b Top 4_Sample30
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b Top 4_Sample30
|
Sample_geo_accession | GSM197297
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197297/suppl/GSM197297.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197298 | GPL1355 |
|
b/b + Fe 3 Days Bottom 1_Sample31
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 3 Days Bottom 1_Sample31
|
Sample_geo_accession | GSM197298
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197298/suppl/GSM197298.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197299 | GPL1355 |
|
b/b + Fe 3 Days Bottom 2_Sample33
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 3 Days Bottom 2_Sample33
|
Sample_geo_accession | GSM197299
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197299/suppl/GSM197299.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197300 | GPL1355 |
|
b/b + Fe 3 Days Bottom 3_Sample35
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 3 Days Bottom 3_Sample35
|
Sample_geo_accession | GSM197300
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197300/suppl/GSM197300.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197301 | GPL1355 |
|
b/b + Fe 3 Days Bottom 4_Sample37
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 3 Days Bottom 4_Sample37
|
Sample_geo_accession | GSM197301
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197301/suppl/GSM197301.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197302 | GPL1355 |
|
b/b + Fe 3 Days Top 1_Sample32
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 3 Days Top 1_Sample32
|
Sample_geo_accession | GSM197302
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197302/suppl/GSM197302.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197303 | GPL1355 |
|
b/b + Fe 3 Days Top 2_Sample34
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 3 Days Top 2_Sample34
|
Sample_geo_accession | GSM197303
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197303/suppl/GSM197303.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197304 | GPL1355 |
|
b/b + Fe 3 Days Top 3_Sample36
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 3 Days Top 3_Sample36
|
Sample_geo_accession | GSM197304
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197304/suppl/GSM197304.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197305 | GPL1355 |
|
b/b + Fe 3 Days Top 4_Sample38
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 3 Days Top 4_Sample38
|
Sample_geo_accession | GSM197305
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197305/suppl/GSM197305.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197306 | GPL1355 |
|
b/b + Fe 6 days Bottom 1_Sample39
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 6 days Bottom 1_Sample39
|
Sample_geo_accession | GSM197306
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197306/suppl/GSM197306.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197307 | GPL1355 |
|
b/b + Fe 6 days Bottom 2_Sample41
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 6 days Bottom 2_Sample41
|
Sample_geo_accession | GSM197307
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197307/suppl/GSM197307.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197308 | GPL1355 |
|
b/b + Fe 6 days Bottom 3_Sample43
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 6 days Bottom 3_Sample43
|
Sample_geo_accession | GSM197308
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197308/suppl/GSM197308.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197309 | GPL1355 |
|
b/b + Fe 6 days Top 1_Sample40
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 6 days Top 1_Sample40
|
Sample_geo_accession | GSM197309
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197309/suppl/GSM197309.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197310 | GPL1355 |
|
b/b + Fe 6 days Top 2_Sample42
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 6 days Top 2_Sample42
|
Sample_geo_accession | GSM197310
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197310/suppl/GSM197310.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197311 | GPL1355 |
|
b/b + Fe 6 days Top 3_Sample44
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Belgrade strain
|
b/b + Fe 6 days Top 3_Sample44
|
Sample_geo_accession | GSM197311
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197311/suppl/GSM197311.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197312 | GPL1355 |
|
+/+ Lower crypt_Sample45
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Lower crypt_Sample45
|
Sample_geo_accession | GSM197312
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197312/suppl/GSM197312.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
GSM197313 | GPL1355 |
|
+/+ Upper crypt_Sample46
|
a 1-cm duodenal segment 1 cm distal from the pylorus was cryostat sectioned at the right angle to the crypt-villus axis and the sections representing the top one-third of villus (top) and the bottom one-third of the villus were pooled for RNA isolation
|
Wistar strain
|
+/+ Upper crypt_Sample46
|
Sample_geo_accession | GSM197313
| Sample_status | Public on May 01 2010
| Sample_submission_date | May 31 2007
| Sample_last_update_date | Jan 26 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Twelve one-month old ++ rats were separated into 3 groups of four rats each. Group 1 rats (samples 1-8) were fed a normal diet and served as control animals. Group 2 (samples 9-16) and Group 3 rats (samples 17-22) were fed a low iron diet and bled 2-3 ml every three days for 4 weeks and served as iron deficient rats (low-iron). Group 3 rats, of which one rat died during anesthesia while undergoing phlebotomy, were fed iron supplementation in drinking water (50 µM Ferric ammonium citrate) for the three days before sacrifice (iron-fed).
| Sample_treatment_protocol_ch1 | For the study of Belgrade rats, eleven bb rats were divided into 3 groups with the 4 rats in Group 1 (samples 23-30) maintained on a normal diet, the 4 rats in Group 2 (samples 31-38) fed iron for 3 days prior to sacrifice, and the 3 rats in Group 3 (samples 39-44) fed iron for 6 days prior to sacrifice. All procedures for the bb rats were the same as described above for the ++ rats. An additional ++ rat (samples 45-46) was similarly analyzed in a separate experiment.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Tri Reagent (Molecular Research Center, Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was used to synthesize double-stranded cDNA using a T7-(dT)24 primer and the Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA). Labeled according to standard Affymetrix protocol.
| Sample_hyb_protocol | Biotinylated cRNA was transcribed, purified, fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix). Hybed according to standard Affymetrix protocol.
| Sample_scan_protocol | After washing and staining, the arrays were scanned using a GeneChip Scanner 3000.
| Sample_data_processing | Pixel intensities were measured, expression signals were analyzed. Data mining and statistical analyses were performed with Data Mining Tool ver 3.0 (Affymetrix) algorithms.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kwo-yih,,Yeh
| Sample_contact_email | Kyeh@lsuhsc.edu
| Sample_contact_phone | 318-675-4967
| Sample_contact_fax | 318-675-4969
| Sample_contact_laboratory | BRI F2-12
| Sample_contact_department | Department of Medicine
| Sample_contact_institute | LSU Health Science Center
| Sample_contact_address | 1501 Kings Highway
| Sample_contact_city | Shreveport
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 71130
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM197nnn/GSM197313/suppl/GSM197313.CEL.gz
| Sample_series_id | GSE7970
| Sample_data_row_count | 31099
| |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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