Result

Search results for the GEO ID: GSE8051

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Title

Source name

Description

Characteristics

GSM198857

GPL341

WKY rep1

Primary branch of saphenous artery pooled from 2-3 16 week old male Wistar-Kyoto rats

16 week old male Wistar-Kyoto rats

Gene expression data from a primary branch of the saphenous artery of 16 week old male Wistar-Kyoto rats

Sample_geo_accession	GSM198857
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	no treatment
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198857/suppl/GSM198857.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198857/suppl/GSM198857.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198857/suppl/GSM198857.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

GSM198858

GPL341

WKY rep2

Primary branch of saphenous artery pooled from 2-3 16 week old male Wistar-Kyoto rats

16 week old male Wistar-Kyoto rats

Gene expression data from a primary branch of the saphenous artery of 16 week old male Wistar-Kyoto rats

Sample_geo_accession	GSM198858
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	no treatment
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198858/suppl/GSM198858.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198858/suppl/GSM198858.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198858/suppl/GSM198858.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

GSM198859

GPL341

WKY rep3

Primary branch of saphenous artery pooled from 2-3 16 week old male Wistar-Kyoto rats

16 week old male Wistar-Kyoto rats

Gene expression data from a primary branch of the saphenous artery of 16 week old male Wistar-Kyoto rats

Sample_geo_accession	GSM198859
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	no treatment
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198859/suppl/GSM198859.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198859/suppl/GSM198859.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198859/suppl/GSM198859.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

GSM198860

GPL341

ACTH treated rep1

Primary branch of saphenous artery pooled from 2-3 16 week old male Wistar-Kyoto rats

16 week old male Wistar-Kyoto rats (ACTH-treated)

Gene expression data from a primary branch of the saphenous artery of 16 week old ACTH-treated male Wistar-Kyoto rats

Sample_geo_accession	GSM198860
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	treated with ACTH (0.1mg/kg/day) subcutaneously, for 4 weeks prior to sampling (i.e. during weeks 12-16 of life)
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198860/suppl/GSM198860.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198860/suppl/GSM198860.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198860/suppl/GSM198860.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

GSM198861

GPL341

ACTH treated rep2

Primary branch of saphenous artery pooled from 2-3 16 week old male Wistar-Kyoto rats

16 week old male Wistar-Kyoto rats (ACTH-treated)

Gene expression data from a primary branch of the saphenous artery of 16 week old ACTH-treated male Wistar-Kyoto rats

Sample_geo_accession	GSM198861
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	treated with ACTH (0.1mg/kg/day) subcutaneously, for 4 weeks prior to sampling (i.e. during weeks 12-16 of life)
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198861/suppl/GSM198861.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198861/suppl/GSM198861.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198861/suppl/GSM198861.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

GSM198862

GPL341

ACTH treated rep3

Primary branch of saphenous artery pooled from 2-3 16 week old male Wistar-Kyoto rats

16 week old male Wistar-Kyoto rats (ACTH-treated)

Gene expression data from a primary branch of the saphenous artery of 16 week old ACTH-treated male Wistar-Kyoto rats

Sample_geo_accession	GSM198862
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	treated with ACTH (0.1mg/kg/day) subcutaneously, for 4 weeks prior to sampling (i.e. during weeks 12-16 of life)
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198862/suppl/GSM198862.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198862/suppl/GSM198862.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198862/suppl/GSM198862.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

GSM198863

GPL341

SHR rep1

Primary branch of saphenous artery pooled from 2-3 16 week old male spontaneously hypertensive (SHR) rats

16 week old male spontaneously hypertensive (SHR) rats

Gene expression data from a primary branch of the saphenous artery of 16 week old spontaneously hypertensive (SHR) rats

Sample_geo_accession	GSM198863
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	no treatment
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198863/suppl/GSM198863.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198863/suppl/GSM198863.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198863/suppl/GSM198863.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

GSM198864

GPL341

SHR rep2

Primary branch of saphenous artery pooled from 2-3 16 week old male spontaneously hypertensive (SHR) rats

16 week old male spontaneously hypertensive (SHR) rats

Gene expression data from a primary branch of the saphenous artery of 16 week old spontaneously hypertensive (SHR) rats

Sample_geo_accession	GSM198864
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	no treatment
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198864/suppl/GSM198864.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198864/suppl/GSM198864.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198864/suppl/GSM198864.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

GSM198865

GPL341

SHR rep3

Primary branch of saphenous artery pooled from 2-3 16 week old male spontaneously hypertensive (SHR) rats

16 week old male spontaneously hypertensive (SHR) rats

Gene expression data from a primary branch of the saphenous artery of 16 week old spontaneously hypertensive (SHR) rats

Sample_geo_accession	GSM198865
Sample_status	Public on Nov 28 2007
Sample_submission_date	Jun 07 2007
Sample_last_update_date	Nov 28 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	no treatment
Sample_growth_protocol_ch1	All RNA samples were obtained from male 16-week old Wistar-Kyoto rats or male 16-week old SHR rats
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rats were anesthetized with ether and killed by decapitation. A primary branch of the saphenous artery was removed, immersed in RNAlater (Ambion) and extraneous material removed in cold phosphate-buffered saline (PBS). Samples (n=3) were taken from 2 or 3 animals from each experimental group. Arteries were snap frozen in liquid nitrogen and processed using the RNeasy Micro Kit (Qiagen), including a 20 min DNAse treatment. RNA quality was monitored on an Agilent 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent). Concentrations of RNA were measured by UV spectrophometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Two cycle preparation of cRNA and hybridization: Sample preparation followed the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II [42]. Double-stranded cDNA was synthesized using oligo(dT) 24-anchored T7 primer, Superscript III (Invitrogen) and 100ng total RNA for the first strand followed by DNA polymerase I and T4 DNA polymerase for the second strand. The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37ºC for 7 h. The cRNA was purified (RNeasy, Qiagen) and used as template for the second round of double-stranded cDNA synthesis with random primers (Invitrogen). The double-stranded cDNA product of second round synthesis was purified by ethanol precipitation and used as template for the second round of in vitro transcription and labeling of cRNA, using the ENZO BioArray HighYield RNA Transcript Labeling kit (Affymetrix) according to the protocol. All components were mixed, incubated at 37ºC for 7 h, and the biotin-labeled cRNA was purified (RNeasy, Qiagen) and fragmented prior to hybridisation to Affymetrix GeneChip RAE230A arrays. Hybridization, washing and staining followed the Affymetrix GeneChip Expression Analysis protocols. Data were collected and analyzed using the program MAS5 (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to each array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. Affymetrix GeneChip Expression Analysis protocols were used throughout.
Sample_scan_protocol	GeneChips were scanned using an Affymetrix 2500A GeneArray Scanner.
Sample_data_processing	Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 150.
Sample_platform_id	GPL341
Sample_contact_name	Stephen,John,Ohms
Sample_contact_email	Stephen.Ohms@anu.edu.au
Sample_contact_phone	61-2-6125-8146
Sample_contact_laboratory	ACRF Biomolecular Resource Facility
Sample_contact_department	John Curtin School of Medical Research
Sample_contact_institute	Australian National University
Sample_contact_address	Garran Road
Sample_contact_city	Canberra
Sample_contact_state	Australian Capital Territory
Sample_contact_zip/postal_code	GPO Box 334, Canberra City
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198865/suppl/GSM198865.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198865/suppl/GSM198865.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198865/suppl/GSM198865.EXP.gz
Sample_series_id	GSE8051
Sample_data_row_count	15923

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