Search results for the GEO ID: GSE8057  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM198888 | GPL8300 |
|
A2780_cis_IC90_pre_1
|
A2780 cells, exposed 2 h to cisplatin at IC90 , pre.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198888
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198888/suppl/GSM198888.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198889 | GPL8300 |
|
A2780_cis_IC90_pre_2
|
A2780 cells, exposed 2 h to cisplatin at IC90 , pre.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198889
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198889/suppl/GSM198889.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198890 | GPL8300 |
|
A2780_control_pre
|
A2780 cells, control , pre.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198890
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198890/suppl/GSM198890.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198891 | GPL8300 |
|
A2780_oxa_IC90_pre_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , pre.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198891
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198891/suppl/GSM198891.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198892 | GPL8300 |
|
A2780_oxa_IC90_pre_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , pre.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198892
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198892/suppl/GSM198892.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198893 | GPL8300 |
|
A2780_cis_IC90_0h_1
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 0h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198893
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198893/suppl/GSM198893.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198894 | GPL8300 |
|
A2780_cis_IC90_0h_2
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 0h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198894
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198894/suppl/GSM198894.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198895 | GPL8300 |
|
A2780_cis_IC90_2h_1
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 2h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198895
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198895/suppl/GSM198895.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198896 | GPL8300 |
|
A2780_cis_IC90_2h_2
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 2h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198896
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198896/suppl/GSM198896.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198897 | GPL8300 |
|
A2780_cis_IC90_6h_1
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 6h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198897
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198897/suppl/GSM198897.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198898 | GPL8300 |
|
A2780_cis_IC90_6h_2
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 6h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198898
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198898/suppl/GSM198898.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198899 | GPL8300 |
|
A2780_cis_IC90_24h
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 24h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198899
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198899/suppl/GSM198899.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198900 | GPL8300 |
|
A2780_oxa_IC90_0h_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 0h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198900
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198900/suppl/GSM198900.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198901 | GPL8300 |
|
A2780_oxa_IC90_0h_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 0h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198901
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198901/suppl/GSM198901.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198902 | GPL8300 |
|
A2780_oxa_IC90_2h_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 2h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198902
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198902/suppl/GSM198902.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198903 | GPL8300 |
|
A2780_oxa_IC90_2h_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 2h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198903
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198903/suppl/GSM198903.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198904 | GPL8300 |
|
A2780_oxa_IC90_6h_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 6h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198904
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198904/suppl/GSM198904.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198905 | GPL8300 |
|
A2780_oxa_IC90_6h_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 6h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198905
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198905/suppl/GSM198905.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198906 | GPL8300 |
|
A2780_oxa_IC90_24h
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 24h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198906
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198906/suppl/GSM198906.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198907 | GPL8300 |
|
A2780_control_0h
|
A2780 cells, control , 0h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198907
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198907/suppl/GSM198907.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198908 | GPL8300 |
|
A2780_control_2h
|
A2780 cells, control , 2h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198908
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198908/suppl/GSM198908.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198909 | GPL8300 |
|
A2780_control_6h
|
A2780 cells, control , 6h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198909
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198909/suppl/GSM198909.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198910 | GPL8300 |
|
A2780_control_16h_1
|
A2780 cells, control , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198910
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198910/suppl/GSM198910.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198911 | GPL8300 |
|
A2780_control_16h_2
|
A2780 cells, control , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198911
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198911/suppl/GSM198911.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198912 | GPL8300 |
|
A2780_control_16h_3
|
A2780 cells, control , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198912
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198912/suppl/GSM198912.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198913 | GPL8300 |
|
A2780_control_16h_4
|
A2780 cells, control , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198913
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198913/suppl/GSM198913.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198914 | GPL8300 |
|
A2780_cis_IC10_16h_1
|
A2780 cells, exposed 2 h to cisplatin at IC10 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198914
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198914/suppl/GSM198914.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198915 | GPL8300 |
|
A2780_cis_IC10_16h_2
|
A2780 cells, exposed 2 h to cisplatin at IC10 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198915
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198915/suppl/GSM198915.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198916 | GPL8300 |
|
A2780_cis_IC25_16h_1
|
A2780 cells, exposed 2 h to cisplatin at IC25 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198916
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198916/suppl/GSM198916.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198917 | GPL8300 |
|
A2780_cis_IC25_16h_2
|
A2780 cells, exposed 2 h to cisplatin at IC25 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198917
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198917/suppl/GSM198917.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198918 | GPL8300 |
|
A2780_cis_IC50_16h_1
|
A2780 cells, exposed 2 h to cisplatin at IC50 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198918
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198918/suppl/GSM198918.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198919 | GPL8300 |
|
A2780_cis_IC50_16h_2
|
A2780 cells, exposed 2 h to cisplatin at IC50 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198919
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198919/suppl/GSM198919.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198920 | GPL8300 |
|
A2780_cis_IC75_16h_1
|
A2780 cells, exposed 2 h to cisplatin at IC75 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198920
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198920/suppl/GSM198920.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198921 | GPL8300 |
|
A2780_cis_IC75_16h_2
|
A2780 cells, exposed 2 h to cisplatin at IC75 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198921
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198921/suppl/GSM198921.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198922 | GPL8300 |
|
A2780_cis_IC90_16h_1
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198922
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198922/suppl/GSM198922.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198923 | GPL8300 |
|
A2780_cis_IC90_16h_2
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198923
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198923/suppl/GSM198923.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198924 | GPL8300 |
|
A2780_cis_IC90_16h_3
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198924
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198924/suppl/GSM198924.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198925 | GPL8300 |
|
A2780_cis_IC90_16h_4
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198925
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198925/suppl/GSM198925.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198926 | GPL8300 |
|
A2780_cis_IC90_16h_5
|
A2780 cells, exposed 2 h to cisplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198926
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198926/suppl/GSM198926.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198927 | GPL8300 |
|
A2780_oxa_IC10_16h_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC10 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198927
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198927/suppl/GSM198927.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198928 | GPL8300 |
|
A2780_oxa_IC10_16h_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC10 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198928
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198928/suppl/GSM198928.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198929 | GPL8300 |
|
A2780_oxa_IC25_16h_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC25 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198929
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198929/suppl/GSM198929.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198930 | GPL8300 |
|
A2780_oxa_IC25_16h_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC25 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198930
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198930/suppl/GSM198930.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198931 | GPL8300 |
|
A2780_oxa_IC50_16h_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC50 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198931
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198931/suppl/GSM198931.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198932 | GPL8300 |
|
A2780_oxa_IC50_16h_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC50 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198932
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198932/suppl/GSM198932.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198933 | GPL8300 |
|
A2780_oxa_IC75_16h_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC75 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198933
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198933/suppl/GSM198933.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198934 | GPL8300 |
|
A2780_oxa_IC75_16h_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC75 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198934
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198934/suppl/GSM198934.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198935 | GPL8300 |
|
A2780_oxa_IC90_16h_1
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198935
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198935/suppl/GSM198935.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198936 | GPL8300 |
|
A2780_oxa_IC90_16h_2
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198936
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198936/suppl/GSM198936.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198937 | GPL8300 |
|
A2780_oxa_IC90_16h_3
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198937
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198937/suppl/GSM198937.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
GSM198938 | GPL8300 |
|
A2780_oxa_IC90_16h_4
|
A2780 cells, exposed 2 h to oxaliplatin at IC90 , 16h.
|
A2780 human ovarian carcinoma cells
|
NA
|
| Sample_geo_accession | GSM198938
| | Sample_status | Public on Jul 01 2007
| | Sample_submission_date | Jun 07 2007
| | Sample_last_update_date | Mar 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | samples were treated with specific growth-inhibitory concentrations of cisplatin or oxaliplatin for 2 hours, then washed three times in PBS and incubated in drug-free media. Cells were harvested and processed at various time.
| | Sample_growth_protocol_ch1 | A2780 is a human ovarian carcinoma cell line generously provided by Dr. Ozols (Fox Chase Cancer Center, Philadelphia, PA). Cells were mycoplasma-free and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine at 37ºC in humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Total RNA from log phase cells (10-15 x 106 cells) was isolated using Qiagen RNeasy kit (Qiagen, Inc.) according to specifications. Poly-A RNA was then isolated using Qiagen Oligotex kit (Qiagen, Inc.) from ~50 ìg total RNA and processed according to Affymetrix specifications (Affymetrix GeneChip Expression Analysis Manual, Affymetrix Inc, Santa Clara, CA). Briefly, double stranded cDNA was synthesized from ~2.5 mg poly-A RNA using the Superscript Choice System (Invitrogen, Grand Island, NY) followed by amplification and biotinylation by in vitro transcription reaction (BioArray High Yield Transcription Labeling Kit, Enzo Diagnostics, Farmingdale, NY).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | polyA RNA was hybridized to Affymetrix U95Av2 GeneChip arrays according to the standard Affymetrix protocol
| | Sample_scan_protocol | Gene chips were scanned using the Affymetrix Genechip Scanner 3000
| | Sample_data_processing | Probe level analysis was performed using Robust Multiarray Average (RMA) method using the Bioconductor package
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Yseult,F,Brun
| | Sample_contact_department | Cancer prevention and epidemiology
| | Sample_contact_institute | Roswell Park Cancer Institute
| | Sample_contact_address | Elm and Carlton St
| | Sample_contact_city | Buffalo
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14263
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM198nnn/GSM198938/suppl/GSM198938.CEL.gz
| | Sample_series_id | GSE8057
| | Sample_data_row_count | 12625
| | |
|
| |
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