Search results for the GEO ID: GSE8096  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM200612 | GPL96 |
|
HMEC S1 day 3 sample 1
|
HMEC S1
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMT3522S1 (HMEC S1)
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200612
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 3 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200612/suppl/GSM200612.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200739 | GPL96 |
|
HMEC S1 day 3 sample 2
|
HMEC S1
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMT3522S1 (HMEC S1)
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200739
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_treatment_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 3 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 3 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200739/suppl/GSM200739.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200740 | GPL96 |
|
HMEC S1 day 5 sample 1
|
HMEC S1
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMT3522S1 (HMEC S1)
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200740
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 5 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200740/suppl/GSM200740.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200741 | GPL96 |
|
HMEC S1 day 5 sample 2
|
HMEC S1
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMT3522S1 (HMEC S1)
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200741
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 5 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200741/suppl/GSM200741.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200742 | GPL96 |
|
HMEC S1 day 7 sample 1
|
HMEC S1
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMT3522S1 (HMEC S1)
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200742
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 7 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200742/suppl/GSM200742.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200743 | GPL96 |
|
HMEC S1 day 7 sample 2
|
HMEC S1
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMT3522S1 (HMEC S1)
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200743
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 7 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200743/suppl/GSM200743.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200748 | GPL96 |
|
HMEC 184 day 3 sample 1
|
HMEC 184
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMEC 184
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200748
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 3 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200748/suppl/GSM200748.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200749 | GPL96 |
|
HMEC 184 day 3 sample 2
|
HMEC 184
|
ioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMEC 184
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200749
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 3 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200749/suppl/GSM200749.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200754 | GPL96 |
|
HMEC 184 day 5 sample 1
|
HMEC 184
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMEC 184
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200754
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 5 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200754/suppl/GSM200754.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200755 | GPL96 |
|
HMEC 184 day 5 sample 2
|
HMEC 184
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BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMEC 184
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We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
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| Sample_geo_accession | GSM200755
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 5 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200755/suppl/GSM200755.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
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GSM200756 | GPL96 |
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HMEC 184 day 7 sample 1
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HMEC 184
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMEC 184
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200756
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 7 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200756/suppl/GSM200756.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
| | |
|
GSM200757 | GPL96 |
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HMEC 184 day 7 sample 2
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HMEC 184
|
BioSourceType: frozen_sample
DevelopmentalStage: adult
OrganismPart: mammary gland
TargetedCellType: human mammary epithelial
Sex: female
Organism: Homo sapiens
CellLine: HMEC 184
|
We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
|
| Sample_geo_accession | GSM200757
| | Sample_status | Public on Jul 12 2007
| | Sample_submission_date | Jun 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Mina Bissell
| | Sample_growth_protocol_ch1 | Cells cultured in a H14 medium (DMEM/F12 containing 250 ng/ml insulin, 10 µg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 x 10-6 M hydrocortisone, 10 ng/ml EGF and 5 µg /ml prolactin) were grown for 7 days in a 3D laminin-rich extracellular matrix (Matrigel BD Biosciences).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was extracted and purified using the QIAGEN RNAeasy kit by following the kit protocol. It was stored at -70C until use.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix in vitro transcription
| | Sample_hyb_protocol | Affymetrix Generic Hybridization
| | Sample_scan_protocol | Affymetrix Scanning
| | Sample_data_processing | Experiments with Affymetrix-present P-call rates of >30% were included in the analysis. Signal values from each of the 22,283 probe sets were calculated by means of robust multi-array analysis (RMA) using Bioconductor. (http://www.bioconductor.org) in the R computing environment. The signal values were inverse log2 transformed and then imported into GeneSpring software (SiliconGenetics); and each array was normalized to its median signal intensity.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Katherine,J,Martin
| | Sample_contact_email | kjm@bioarray.us
| | Sample_contact_institute | Bioarray Consulting
| | Sample_contact_address | 61 Carleton Rd
| | Sample_contact_city | Belmont
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02478
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://bioarray.us/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM200nnn/GSM200757/suppl/GSM200757.CEL.gz
| | Sample_series_id | GSE8096
| | Sample_data_row_count | 22283
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