Search results for the GEO ID: GSE8171  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM202262 | GPL339 |
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Py-4-1_pcDNA_1
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Mouse hemangioendothelioma (Py-4-1 cell line)
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Py-4-1, a hemangioma derived cell line established from a Py-4 mouse cell line carrying polyoma virus early region, transfected with a control pcDNA vector.
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control-transfected Py-4-1 cell clone
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| Sample_geo_accession | GSM202262
| | Sample_status | Public on Aug 04 2008
| | Sample_submission_date | Jun 19 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolate total cellular RNA with 1 ml of the Trizol reagent (Invitrogen) after washing one 80-90 % confluent 10 cm culture dish with PBS. Homogenize the sample using a cell scraper and add 0.2 ml of chloroform in a 1.5 ml tube. Shake the sample vigorously for 15 seconds and allow standing for 5 minutes. Centrifuge the resulting mixture at 12,000 x g for 15 minutes at 4 degree Celsius. Transfer the aqueous phase to a fresh tube and add 0.5 ml of iso-propanol and mix. Allow the sample to stand for 5 minutes at room temperature and centrifuge at 12,000 x g for 10 minutes at 4 degrees Celsius. Remove the supernatant and wash the RNA pellet by adding 1 ml of 75% ethanol. Vortex the sample and then centrifuge at 12,000 x g for 5 minutes at 4 degrees Celsius. Remove the ethanol and briefly dry the RNA pellet for 5 minutes and add 50 micro-liter of RNAase/DNAase free water and mix by repeated pipetting until RNA pellet is fully dissolved.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 10 micrograms of total RNA was processed to produce biotinylated cRNA targets.
| | Sample_hyb_protocol | Standard Affymetrix procedures were followed for hybridization and scanning of the arrays.
| | Sample_scan_protocol | Standard Affymetrix procedures were followed for hybridization and scanning of the arrays.
| | Sample_data_processing | Standard Affymetrix analysis using the MAS5 method.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jay,Woo,Shin
| | Sample_contact_email | jay.shin@gsc.riken.jp
| | Sample_contact_laboratory | Michael Detmar
| | Sample_contact_department | Institute of Pharmaceutical Sciences
| | Sample_contact_institute | ETH Zurich
| | Sample_contact_address | Wolfgang-Paulistrasse 10; HCI H394
| | Sample_contact_city | Zurich
| | Sample_contact_state | Zurich
| | Sample_contact_zip/postal_code | 8093
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM202nnn/GSM202262/suppl/GSM202262.CEL.gz
| | Sample_series_id | GSE8171
| | Sample_data_row_count | 22690
| | |
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GSM202263 | GPL339 |
|
Py-4-1_pcDNA_2
|
Mouse hemangioendothelioma (Py-4-1 cell line)
|
Py-4-1, a hemangioma derived cell line established from a Py-4 mouse cell line carrying polyoma virus early region, transfected with a control pcDNA vector.
|
control-transfected Py-4-1 cell clone
|
| Sample_geo_accession | GSM202263
| | Sample_status | Public on Aug 04 2008
| | Sample_submission_date | Jun 19 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolate total cellular RNA with 1 ml of the Trizol reagent (Invitrogen) after washing one 80-90 % confluent 10 cm culture dish with PBS. Homogenize the sample using a cell scraper and add 0.2 ml of chloroform in a 1.5 ml tube. Shake the sample vigorously for 15 seconds and allow standing for 5 minutes. Centrifuge the resulting mixture at 12,000 x g for 15 minutes at 4 degree Celsius. Transfer the aqueous phase to a fresh tube and add 0.5 ml of iso-propanol and mix. Allow the sample to stand for 5 minutes at room temperature and centrifuge at 12,000 x g for 10 minutes at 4 degrees Celsius. Remove the supernatant and wash the RNA pellet by adding 1 ml of 75% ethanol. Vortex the sample and then centrifuge at 12,000 x g for 5 minutes at 4 degrees Celsius. Remove the ethanol and briefly dry the RNA pellet for 5 minutes and add 50 micro-liter of RNAase/DNAase free water and mix by repeated pipetting until RNA pellet is fully dissolved.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 10 micrograms of total RNA was processed to produce biotinylated cRNA targets.
| | Sample_hyb_protocol | Standard Affymetrix procedures were followed for hybridization and scanning of the arrays.
| | Sample_scan_protocol | Standard Affymetrix procedures were followed for hybridization and scanning of the arrays.
| | Sample_data_processing | Standard Affymetrix analysis using the MAS5 method.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jay,Woo,Shin
| | Sample_contact_email | jay.shin@gsc.riken.jp
| | Sample_contact_laboratory | Michael Detmar
| | Sample_contact_department | Institute of Pharmaceutical Sciences
| | Sample_contact_institute | ETH Zurich
| | Sample_contact_address | Wolfgang-Paulistrasse 10; HCI H394
| | Sample_contact_city | Zurich
| | Sample_contact_state | Zurich
| | Sample_contact_zip/postal_code | 8093
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM202nnn/GSM202263/suppl/GSM202263.CEL.gz
| | Sample_series_id | GSE8171
| | Sample_data_row_count | 22690
| | |
|
GSM202264 | GPL339 |
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Py-4-1_pcDNA/Prox1_2
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Mouse hemangioendothelioma (Py-4-1 cell line)
|
Py-4-1, a hemangioma derived cell line established from a Py-4 transgenic mouse cell line carrying polyoma virus early region, transfected with a pcDNA/Prox1 vector.
|
stable Prox1-expressing Py-4-1 cell clone
|
| Sample_geo_accession | GSM202264
| | Sample_status | Public on Aug 04 2008
| | Sample_submission_date | Jun 19 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolate total cellular RNA with 1 ml of the Trizol reagent (Invitrogen) after washing one 80-90 % confluent 10 cm culture dish with PBS. Homogenize the sample using a cell scraper and add 0.2 ml of chloroform in a 1.5 ml tube. Shake the sample vigorously for 15 seconds and allow standing for 5 minutes. Centrifuge the resulting mixture at 12,000 x g for 15 minutes at 4 degree Celsius. Transfer the aqueous phase to a fresh tube and add 0.5 ml of iso-propanol and mix. Allow the sample to stand for 5 minutes at room temperature and centrifuge at 12,000 x g for 10 minutes at 4 degrees Celsius. Remove the supernatant and wash the RNA pellet by adding 1 ml of 75% ethanol. Vortex the sample and then centrifuge at 12,000 x g for 5 minutes at 4 degrees Celsius. Remove the ethanol and briefly dry the RNA pellet for 5 minutes and add 50 micro-liter of RNAase/DNAase free water and mix by repeated pipetting until RNA pellet is fully dissolved.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 10 micrograms of total RNA was processed to produce biotinylated cRNA targets.
| | Sample_hyb_protocol | Standard Affymetrix procedures were followed for hybridization and scanning of the arrays.
| | Sample_scan_protocol | Standard Affymetrix procedures were followed for hybridization and scanning of the arrays.
| | Sample_data_processing | Standard Affymetrix analysis using the MAS5 method.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jay,Woo,Shin
| | Sample_contact_email | jay.shin@gsc.riken.jp
| | Sample_contact_laboratory | Michael Detmar
| | Sample_contact_department | Institute of Pharmaceutical Sciences
| | Sample_contact_institute | ETH Zurich
| | Sample_contact_address | Wolfgang-Paulistrasse 10; HCI H394
| | Sample_contact_city | Zurich
| | Sample_contact_state | Zurich
| | Sample_contact_zip/postal_code | 8093
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM202nnn/GSM202264/suppl/GSM202264.CEL.gz
| | Sample_series_id | GSE8171
| | Sample_data_row_count | 22690
| | |
|
GSM202265 | GPL339 |
|
Py-4-1_pcDNA/Prox1_1
|
Mouse hemangioendothelioma (Py-4-1 cell line)
|
Py-4-1, a hemangioma derived cell line established from a Py-4 transgenic mouse line carrying polyoma virus early region, transfected with a pcDNA/Prox1 vector.
|
stable Prox1-expressing Py-4-1 cell clone
|
| Sample_geo_accession | GSM202265
| | Sample_status | Public on Aug 04 2008
| | Sample_submission_date | Jun 19 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolate total cellular RNA with 1 ml of the Trizol reagent (Invitrogen) after washing one 80-90 % confluent 10 cm culture dish with PBS. Homogenize the sample using a cell scraper and add 0.2 ml of chloroform in a 1.5 ml tube. Shake the sample vigorously for 15 seconds and allow standing for 5 minutes. Centrifuge the resulting mixture at 12,000 x g for 15 minutes at 4 degree Celsius. Transfer the aqueous phase to a fresh tube and add 0.5 ml of iso-propanol and mix. Allow the sample to stand for 5 minutes at room temperature and centrifuge at 12,000 x g for 10 minutes at 4 degrees Celsius. Remove the supernatant and wash the RNA pellet by adding 1 ml of 75% ethanol. Vortex the sample and then centrifuge at 12,000 x g for 5 minutes at 4 degrees Celsius. Remove the ethanol and briefly dry the RNA pellet for 5 minutes and add 50 micro-liter of RNAase/DNAase free water and mix by repeated pipetting until RNA pellet is fully dissolved.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 10 micrograms of total RNA was processed to produce biotinylated cRNA targets.
| | Sample_hyb_protocol | Standard Affymetrix procedures were followed for hybridization and scanning of the arrays.
| | Sample_scan_protocol | Standard Affymetrix procedures were followed for hybridization and scanning of the arrays.
| | Sample_data_processing | Standard Affymetrix analysis using the MAS5 method.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jay,Woo,Shin
| | Sample_contact_email | jay.shin@gsc.riken.jp
| | Sample_contact_laboratory | Michael Detmar
| | Sample_contact_department | Institute of Pharmaceutical Sciences
| | Sample_contact_institute | ETH Zurich
| | Sample_contact_address | Wolfgang-Paulistrasse 10; HCI H394
| | Sample_contact_city | Zurich
| | Sample_contact_state | Zurich
| | Sample_contact_zip/postal_code | 8093
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM202nnn/GSM202265/suppl/GSM202265.CEL.gz
| | Sample_series_id | GSE8171
| | Sample_data_row_count | 22690
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