Result

Search results for the GEO ID: GSE8238

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM203966

GPL1355

ovary_cultured_control_rep1

0-day rat ovary cultured 2 days

ovaries dissected from 0-day old Sprague-Dawley rats and cleaned of ovarian bursa.

Chip ID=EricBK1P47-C-1-071905. RNA from zero-day old rat ovaries cultured two days as controls with no treatment.

Sample_geo_accession	GSM203966
Sample_status	Public on Jun 22 2007
Sample_submission_date	Jun 22 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female rat pups less than eight hours old were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Newborn rat ovaries were dissected from freshly euthanized rat pups. Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ug/ml transferrin, 200 ng/ml insulin (human recombinant, Sigma), and 0.05 mg/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Ovaries were randomly assigned to treatment groups with 2-3 ovaries per floating filter in each well. Ovaries were un-treated and used as controls. Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two pooled independent RNA samples were analysed for each treatment group (control or P4-treated ovaries cultured for two days).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA) where raw data were filtered for flags (present/absent calls), expression level, fold change and confidence (t-test p-value) with no multiple testing correction.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM203nnn/GSM203966/suppl/GSM203966.CEL.gz
Sample_series_id	GSE8238
Sample_data_row_count	31099

GSM203967

GPL1355

ovary_cultured_control_rep2

0-day rat ovary cultured 2 days

ovaries dissected from 0-day old Sprague-Dawley rats and cleaned of ovarian bursa.

Chip ID=EricBK1P-C-2-071905. RNA from zero-day old rat ovaries cultured two days as controls with no treatment.

Sample_geo_accession	GSM203967
Sample_status	Public on Jun 22 2007
Sample_submission_date	Jun 22 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female rat pups less than eight hours old were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Newborn rat ovaries were dissected from freshly euthanized rat pups. Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ug/ml transferrin, 200 ng/ml insulin (human recombinant, Sigma), and 0.05 mg/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Ovaries were randomly assigned to treatment groups with 2-3 ovaries per floating filter in each well. Ovaries were un-treated and used as controls. Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two pooled independent RNA samples were analysed for each treatment group (control or P4-treated ovaries cultured for two days).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA) where raw data were filtered for flags (present/absent calls), expression level, fold change and confidence (t-test p-value) with no multiple testing correction.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM203nnn/GSM203967/suppl/GSM203967.CEL.gz
Sample_series_id	GSE8238
Sample_data_row_count	31099

GSM203968

GPL1355

ovary_cultured_progesterone_rep1

0-day rat ovary cultured 2 days

ovaries dissected from 0-day old Sprague-Dawley rats and cleaned of ovarian bursa.

Chip ID=EricBK1P47-P4-1-071905. RNA from zero-day old rat ovaries cultured two days with progesterone treatment.

Sample_geo_accession	GSM203968
Sample_status	Public on Jun 22 2007
Sample_submission_date	Jun 22 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female rat pups less than eight hours old were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Newborn rat ovaries were dissected from freshly euthanized rat pups. Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ug/ml transferrin, 200 ng/ml insulin (human recombinant, Sigma), and 0.05 mg/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Ovaries were randomly assigned to treatment groups with 2-3 ovaries per floating filter in each well. Ovaries were treated with progesterone (P4) (Sigma Chemical Co., St. Louis, MO) at 10-6M. Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two pooled independent RNA samples were analysed for each treatment group (control or P4-treated ovaries cultured for two days).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA) where raw data were filtered for flags (present/absent calls), expression level, fold change and confidence (t-test p-value) with no multiple testing correction.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM203nnn/GSM203968/suppl/GSM203968.CEL.gz
Sample_series_id	GSE8238
Sample_data_row_count	31099

GSM203969

GPL1355

ovary_cultured_progesterone_rep2

0-day rat ovary cultured 2 days

ovaries dissected from 0-day old Sprague-Dawley rats and cleaned of ovarian bursa.

Chip ID=EricBK1P35-P4-2-071905. RNA from zero-day old rat ovaries cultured two days with progesterone treatment.

Sample_geo_accession	GSM203969
Sample_status	Public on Jun 22 2007
Sample_submission_date	Jun 22 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female rat pups less than eight hours old were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Newborn rat ovaries were dissected from freshly euthanized rat pups. Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ug/ml transferrin, 200 ng/ml insulin (human recombinant, Sigma), and 0.05 mg/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Ovaries were randomly assigned to treatment groups with 2-3 ovaries per floating filter in each well. Ovaries were treated with progesterone (P4) (Sigma Chemical Co., St. Louis, MO) at 10-6M. Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two pooled independent RNA samples were analysed for each treatment group (control or P4-treated ovaries cultured for two days).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA) where raw data were filtered for flags (present/absent calls), expression level, fold change and confidence (t-test p-value) with no multiple testing correction.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM203nnn/GSM203969/suppl/GSM203969.CEL.gz
Sample_series_id	GSE8238
Sample_data_row_count	31099

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