Search results for the GEO ID: GSE8444  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM209885 | GPL341 |
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RTEC11 cells cultured at permissive temperature for 3 days-1
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Rat tracheal epithelial cell line RTEC11
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Rat tracheal epithelial cell line RTEC11
|
A rat conditionally immortalized tracheal epithelial cell line, RTEC11, derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen was used. RTEC11 cells were cultured at permissive (33°C) temperature for 3 days.
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| Sample_geo_accession | GSM209885
| | Sample_status | Public on Jul 17 2007
| | Sample_submission_date | Jul 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Hybridization Protocol: After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL341
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM209nnn/GSM209885/suppl/GSM209885.CEL.gz
| | Sample_series_id | GSE8444
| | Sample_data_row_count | 15923
| | |
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GSM209886 | GPL341 |
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RTEC11 cells cultured at permissive temperature for 3 days-2
|
Rat tracheal epithelial cell line RTEC11
|
Rat tracheal epithelial cell line RTEC11
|
A rat conditionally immortalized tracheal epithelial cell line, RTEC11, derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen was used. RTEC11 cells were cultured at permissive (33°C) temperature for 3 days.
|
| Sample_geo_accession | GSM209886
| | Sample_status | Public on Jul 17 2007
| | Sample_submission_date | Jul 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Hybridization Protocol: After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL341
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM209nnn/GSM209886/suppl/GSM209886.CEL.gz
| | Sample_series_id | GSE8444
| | Sample_data_row_count | 15923
| | |
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GSM209887 | GPL341 |
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RTEC11 cells cultured at nonpermissive temperature for 3 days-1
|
Rat tracheal epithelial cell line RTEC11
|
Rat tracheal epithelial cell line RTEC11
|
A rat conditionally immortalized tracheal epithelial cell line, RTEC11, derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen was used. RTEC11 cells were cultured at nonpermissive (39°C) temperature for 3 days.
|
| Sample_geo_accession | GSM209887
| | Sample_status | Public on Jul 17 2007
| | Sample_submission_date | Jul 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Hybridization Protocol: After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL341
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM209nnn/GSM209887/suppl/GSM209887.CEL.gz
| | Sample_series_id | GSE8444
| | Sample_data_row_count | 15923
| | |
|
GSM209888 | GPL341 |
|
RTEC11 cells cultured at nonpermissive temperature for 3 days-2
|
Rat tracheal epithelial cell line RTEC11
|
Rat tracheal epithelial cell line RTEC11
|
A rat conditionally immortalized tracheal epithelial cell line, RTEC11, derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen was used. RTEC11 cells were cultured at nonpermissive (39°C) temperature for 3 days.
|
| Sample_geo_accession | GSM209888
| | Sample_status | Public on Jul 17 2007
| | Sample_submission_date | Jul 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Hybridization Protocol: After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL341
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM209nnn/GSM209888/suppl/GSM209888.CEL.gz
| | Sample_series_id | GSE8444
| | Sample_data_row_count | 15923
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