Result

Search results for the GEO ID: GSE8471

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GSM ID

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Title

Source name

Description

Characteristics

GSM210180

GPL570

MCF7 Control

breast epithelial cell, adherent, without stimulus, control

Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian

Gene expression induced by growth hormone (HRG) stimulation

Sample_geo_accession	GSM210180
Sample_status	Public on Feb 08 2008
Sample_submission_date	Jul 13 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
Sample_growth_protocol_ch1	Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
Sample_hyb_protocol	Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (section2, 2.2.p3-8 & 2.3.p3-14)
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Mariko,,Okada
Sample_contact_email	marikoh@rcai.riken.jp
Sample_contact_laboratory	Laboratory for Cellular Systems Modeling
Sample_contact_institute	RIKEN RCAI
Sample_contact_address	W518, 1-7-22, Suehiro-cho, Tsurumi-ku
Sample_contact_city	Yokohama
Sample_contact_zip/postal_code	230-0045
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM210nnn/GSM210180/suppl/GSM210180.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM210nnn/GSM210180/suppl/GSM210180.EXP.gz
Sample_series_id	GSE8471
Sample_data_row_count	54675

GSM210181

GPL570

MCF7 HRG 2h

breast epithelial cell, adherent, HRG, 10nM, 2h

Gene expression induced by growth hormone (HRG) stimulation

Sample_geo_accession	GSM210181
Sample_status	Public on Feb 08 2008
Sample_submission_date	Jul 13 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
Sample_growth_protocol_ch1	Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
Sample_hyb_protocol	Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (section2, 2.2.p3-8 & 2.3.p3-14)
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Mariko,,Okada
Sample_contact_email	marikoh@rcai.riken.jp
Sample_contact_laboratory	Laboratory for Cellular Systems Modeling
Sample_contact_institute	RIKEN RCAI
Sample_contact_address	W518, 1-7-22, Suehiro-cho, Tsurumi-ku
Sample_contact_city	Yokohama
Sample_contact_zip/postal_code	230-0045
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM210nnn/GSM210181/suppl/GSM210181.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM210nnn/GSM210181/suppl/GSM210181.EXP.gz
Sample_series_id	GSE8471
Sample_data_row_count	54675

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