Search results for the GEO ID: GSE8596 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM213306 | GPL570 |
|
EES-1
|
EES-1
|
EFT cell line expressing EWS/FLI1
|
Gene expression data from a EFT cell line expressing EWS/FLI1 typeI
|
Sample_geo_accession | GSM213306
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213306/suppl/GSM213306.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213306/suppl/GSM213306.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213306/suppl/GSM213306.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213307 | GPL570 |
|
SCCH196
|
SCCH196
|
EFT cell line expressing EWS/FLI1
|
Gene expression data from a EFT cell line expressing EWS/FLI1 typeI
|
Sample_geo_accession | GSM213307
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213307/suppl/GSM213307.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213307/suppl/GSM213307.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213307/suppl/GSM213307.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213308 | GPL570 |
|
RD-ES
|
RD-ES
|
EFT cell line expressing EWS/FLI1
|
Gene expression data from a EFT cell line expressing EWS/FLI1 typeII
|
Sample_geo_accession | GSM213308
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213308/suppl/GSM213308.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213308/suppl/GSM213308.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213308/suppl/GSM213308.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213309 | GPL570 |
|
SK-ES1
|
SK-ES1
|
EFT cell line expressing EWS/FLI1
|
Gene expression data from a EFT cell line expressing EWS/FLI1 typeII
|
Sample_geo_accession | GSM213309
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213309/suppl/GSM213309.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213309/suppl/GSM213309.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213309/suppl/GSM213309.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213310 | GPL570 |
|
NCR-EW2
|
NCR-EW2
|
EFT cell line expressing EWS/FLI1
|
Gene expression data from a EFT cell line expressing EWS/FLI1 typeII
|
Sample_geo_accession | GSM213310
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213310/suppl/GSM213310.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213310/suppl/GSM213310.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213310/suppl/GSM213310.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213311 | GPL570 |
|
NCR-EW3
|
NCR-EW3
|
EFT cell line expressing EWS/E1AF
|
Gene expression data from a EFT cell line expressing EWS/E1AF
|
Sample_geo_accession | GSM213311
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213311/suppl/GSM213311.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213311/suppl/GSM213311.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213311/suppl/GSM213311.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213312 | GPL570 |
|
W-ES
|
W-ES
|
EFT cell line expressing EWS/ERG
|
Gene expression data from a EFT cell line expressing EWS/ERG
|
Sample_geo_accession | GSM213312
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213312/suppl/GSM213312.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213312/suppl/GSM213312.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213312/suppl/GSM213312.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213313 | GPL570 |
|
NB69
|
NB69
|
NB cell line
|
Gene expression data from a NB cell line
|
Sample_geo_accession | GSM213313
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213313/suppl/GSM213313.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213313/suppl/GSM213313.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213313/suppl/GSM213313.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213314 | GPL570 |
|
NB9
|
NB9
|
NB cell line
|
Gene expression data from a NB cell line
|
Sample_geo_accession | GSM213314
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213314/suppl/GSM213314.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213314/suppl/GSM213314.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213314/suppl/GSM213314.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213315 | GPL570 |
|
GOTO
|
GOTO
|
NB cell line
|
Gene expression data from a NB cell line
|
Sample_geo_accession | GSM213315
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213315/suppl/GSM213315.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213315/suppl/GSM213315.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213315/suppl/GSM213315.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
| |
|
GSM213316 | GPL570 |
|
NRS-1
|
NRS-1
|
RMS cell line
|
Gene expression data from a RMS cell line
|
Sample_geo_accession | GSM213316
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213316/suppl/GSM213316.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213316/suppl/GSM213316.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213316/suppl/GSM213316.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
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GSM213317 | GPL570 |
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UET-13
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UET-13
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UET-13 cells are derived from human bone marrow stromal cells by prolonging the life span by retroviral transgenes, hTERT and E7
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Gene expression data from UET-13 cells
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Sample_geo_accession | GSM213317
| Sample_status | Public on Jul 27 2007
| Sample_submission_date | Jul 26 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoユs modified Eagleユs medium (DMEM) with 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshitaka,,Miyagawa
| Sample_contact_email | miyayoshi0007@yahoo.co.jp
| Sample_contact_institute | National Research Institute for Child Health and Development
| Sample_contact_address | Setagaya-ku
| Sample_contact_city | Okura
| Sample_contact_zip/postal_code | 157-8535
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213317/suppl/GSM213317.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213317/suppl/GSM213317.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213317/suppl/GSM213317.EXP.gz
| Sample_series_id | GSE8596
| Sample_data_row_count | 54675
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