Search results for the GEO ID: GSE8597  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM213318 | GPL570 |
|
MCF7_CHX_E2_24h_rep1
|
MCF7 breast cancer cells
|
MCF7 CHX+E2
|
MCF7 pre-treated for 1h with cycloheximide, treated with E2 for 24h
|
| Sample_geo_accession | GSM213318
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213318/suppl/GSM213318.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213319 | GPL570 |
|
MCF7_CHX_E2_24h_rep2
|
MCF7 breast cancer cells
|
MCF7 CHX+E2
|
MCF7 pre-treated for 1h with cycloheximide, treated with E2 for 24h
|
| Sample_geo_accession | GSM213319
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213319/suppl/GSM213319.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213320 | GPL570 |
|
MCF7_CHX_E2_24h_rep3
|
MCF7 breast cancer cells
|
MCF7 CHX+E2
|
MCF7 pre-treated for 1h with cycloheximide, treated with E2 for 24h
|
| Sample_geo_accession | GSM213320
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213320/suppl/GSM213320.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213321 | GPL570 |
|
MCF7_CHX_E2_24h_rep4
|
MCF7 breast cancer cells
|
MCF7 CHX+E2
|
MCF7 pre-treated for 1h with cycloheximide, treated with E2 for 24h
|
| Sample_geo_accession | GSM213321
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213321/suppl/GSM213321.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213322 | GPL570 |
|
MCF7_CHX_EtOH_24h_rep1
|
MCF7 breast cancer cells
|
MCF7 CHX+EtOH
|
MCF7 pre-treated for 1h with cycloheximide, treated with vehicule for 24h
|
| Sample_geo_accession | GSM213322
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213322/suppl/GSM213322.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213323 | GPL570 |
|
MCF7_CHX_EtOH_24h_rep2
|
MCF7 breast cancer cells
|
MCF7 CHX+EtOH
|
MCF7 pre-treated for 1h with cycloheximide, treated with vehicule for 24h
|
| Sample_geo_accession | GSM213323
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213323/suppl/GSM213323.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213324 | GPL570 |
|
MCF7_CHX_EtOH_24h_rep3
|
MCF7 breast cancer cells
|
MCF7 CHX+EtOH
|
MCF7 pre-treated for 1h with cycloheximide, treated with vehicule for 24h
|
| Sample_geo_accession | GSM213324
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213324/suppl/GSM213324.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213325 | GPL570 |
|
MCF7_CHX_EtOH_24h_rep4
|
MCF7 breast cancer cells
|
MCF7 CHX+EtOH
|
MCF7 pre-treated for 1h with cycloheximide, treated with vehicule for 24h
|
| Sample_geo_accession | GSM213325
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213325/suppl/GSM213325.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213326 | GPL570 |
|
MCF7_E2_24h_rep1
|
MCF7 breast cancer cells
|
MCF7 DMSO+E2
|
MCF7 treated with E2 for 24h
|
| Sample_geo_accession | GSM213326
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213326/suppl/GSM213326.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213327 | GPL570 |
|
MCF7_E2_24h_rep2
|
MCF7 breast cancer cells
|
MCF7 DMSO+E2
|
MCF7 treated with E2 for 24h
|
| Sample_geo_accession | GSM213327
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213327/suppl/GSM213327.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213328 | GPL570 |
|
MCF7_E2_24h_rep3
|
MCF7 breast cancer cells
|
MCF7 DMSO+E2
|
MCF7 treated with E2 for 24h
|
| Sample_geo_accession | GSM213328
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213328/suppl/GSM213328.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213329 | GPL570 |
|
MCF7_E2_24h_rep4
|
MCF7 breast cancer cells
|
MCF7 DMSO+E2
|
MCF7 treated with E2 for 24h
|
| Sample_geo_accession | GSM213329
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213329/suppl/GSM213329.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213330 | GPL570 |
|
MCF7_EtOH_24h_rep1
|
MCF7 breast cancer cells
|
MCF7 DMSO+EtOH
|
MCF7 treated with vehicule for 24h
|
| Sample_geo_accession | GSM213330
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213330/suppl/GSM213330.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213331 | GPL570 |
|
MCF7_EtOH_24h_rep2
|
MCF7 breast cancer cells
|
MCF7 DMSO+EtOH
|
MCF7 treated with vehicule for 24h
|
| Sample_geo_accession | GSM213331
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213331/suppl/GSM213331.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213332 | GPL570 |
|
MCF7_EtOH_24h_rep3
|
MCF7 breast cancer cells
|
MCF7 DMSO+EtOH
|
MCF7 treated with vehicule for 24h
|
| Sample_geo_accession | GSM213332
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213332/suppl/GSM213332.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
GSM213333 | GPL570 |
|
MCF7_EtOH_24h_rep4
|
MCF7 breast cancer cells
|
MCF7 DMSO+EtOH
|
MCF7 treated with vehicule for 24h
|
| Sample_geo_accession | GSM213333
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Jul 26 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Pre-treatments with cycloheximide (10 microg/ml, Sigma) or vehicle DMSO (0.1%) were initiated 1 h before hormonal treatment with 17{beta}-estradiol (E2, 25 nM, Sigma) or vehicle (0.1% ethanol) for 24h.
| | Sample_growth_protocol_ch1 | MCF-7 breast carcinoma cells were maintained in {alpha}-minimal Eagle’s medium ({alpha}-MEM) (Wisent, St-Bruno, QC, Canada) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, Oakville, On, Canada). Three days before experiments, cells were switched to phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 10% charcoal-treated FBS, 1% sodium pyruvate (Wisent), 1% penicillin/streptomycin (Wisent) and 1% L-glutamine (Wisent). Cells were seeded in 10 cm plates at a density such that near-confluency is obtained at the end of the treatment. The day before hormonal stimulation, the medium was changed to phenol red-free DMEM supplemented with 0,5% charcoal-treated FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After completion of hormonal treatments, medium was completely removed and cells were collected in 1 ml of TRI-Reagent (Sigma). Total RNA was extracted as recommended by the manufacturer and further purified with RNeasy MinElute Cleanup Kit (QIAgen, Mississauga, On, Canada).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | R/Bioconductor RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Laperriere
| | Sample_contact_email | david.laperriere@umontreal.ca
| | Sample_contact_laboratory | Dr Sylvie Mader
| | Sample_contact_institute | IRIC - Institut de recherche en immunologie et en cancerologie
| | Sample_contact_address | 2950, chemin de Polytechnique
| | Sample_contact_city | Montreal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H3T 1J4
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://iric.ca/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM213nnn/GSM213333/suppl/GSM213333.CEL.gz
| | Sample_series_id | GSE8597
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
