Search results for the GEO ID: GSE8658  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM214734 | GPL570 |
|
MC1a
|
monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 26 yr; weight: 71 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214734
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214734/suppl/GSM214734.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214737 | GPL570 |
|
MC1b
|
monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 19 yr; weight: 65 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214737
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214737/suppl/GSM214737.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214738 | GPL570 |
|
MC2a
|
monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 34 yr; weight: 105 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214738
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214738/suppl/GSM214738.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214739 | GPL570 |
|
MC2b
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 47 yr; weight: 80 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214739
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214739/suppl/GSM214739.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214740 | GPL570 |
|
MC3a
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 89 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214740
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214740/suppl/GSM214740.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214741 | GPL570 |
|
MC3b
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214741
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214741/suppl/GSM214741.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214742 | GPL570 |
|
MC4a
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 79 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214742
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214742/suppl/GSM214742.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214743 | GPL570 |
|
MC4b
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 21 yr; weight: 110 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214743
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214743/suppl/GSM214743.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214744 | GPL570 |
|
MC5a
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 95 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214744
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214744/suppl/GSM214744.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214745 | GPL570 |
|
MC5b
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 82 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214745
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214745/suppl/GSM214745.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214746 | GPL570 |
|
MC6a
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 52 yr; weight: 75 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214746
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214746/suppl/GSM214746.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214749 | GPL570 |
|
MC6b
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 73 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214749
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | freshly isolated monocytes
| | Sample_growth_protocol_ch1 | freshly isolated monocytes
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214749/suppl/GSM214749.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214750 | GPL570 |
|
6h DC1
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 26 yr; weight: 71 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214750
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214750/suppl/GSM214750.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214751 | GPL570 |
|
6h DC1 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 26 yr; weight: 71 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214751
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214751/suppl/GSM214751.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214752 | GPL570 |
|
6h DC2
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 34 yr; weight: 105 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214752
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214752/suppl/GSM214752.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214753 | GPL570 |
|
6h DC2 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 34 yr; weight: 105 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214753
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with 1uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214753/suppl/GSM214753.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214754 | GPL570 |
|
6h DC3
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 89 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214754
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214754/suppl/GSM214754.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214755 | GPL570 |
|
6h DC3 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 89 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214755
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214755/suppl/GSM214755.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214756 | GPL570 |
|
6h DC4
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 79 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214756
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214756/suppl/GSM214756.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214757 | GPL570 |
|
6h DC4 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 79 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214757
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214757/suppl/GSM214757.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214763 | GPL570 |
|
6h DC4 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 79 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214763
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 1uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214763/suppl/GSM214763.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214764 | GPL570 |
|
6h DC5
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 95 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214764
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214764/suppl/GSM214764.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214765 | GPL570 |
|
6h DC5 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 95 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214765
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214765/suppl/GSM214765.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214766 | GPL570 |
|
6h DC5 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 95 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214766
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214766/suppl/GSM214766.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214767 | GPL570 |
|
6h DC6
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 52 yr; weight: 75 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214767
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214767/suppl/GSM214767.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214768 | GPL570 |
|
6h DC6 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 52 yr; weight: 75 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214768
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214768/suppl/GSM214768.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214769 | GPL570 |
|
6h DC6 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 52 yr; weight: 75 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214769
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 6 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214769/suppl/GSM214769.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214770 | GPL570 |
|
24h DC1
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 26 yr; weight: 71 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM214770
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214770/suppl/GSM214770.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214771 | GPL570 |
|
24h DC1 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 26 yr; weight: 71 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM214771
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214771/suppl/GSM214771.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214772 | GPL570 |
|
24h DC2
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 34 yr; weight: 105 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM214772
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214772/suppl/GSM214772.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214773 | GPL570 |
|
24h DC2 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 34 yr; weight: 105 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214773
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214773/suppl/GSM214773.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214774 | GPL570 |
|
24h DC3
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 89 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM214774
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214774/suppl/GSM214774.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214775 | GPL570 |
|
24h DC3 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 89 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM214775
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214775/suppl/GSM214775.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214776 | GPL570 |
|
24h DC4
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 79 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM214776
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214776/suppl/GSM214776.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214777 | GPL570 |
|
24h DC4 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 79 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214777
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214777/suppl/GSM214777.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214778 | GPL570 |
|
24h DC4 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 79 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM214778
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214778/suppl/GSM214778.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214779 | GPL570 |
|
24h DC5
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 95 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214779
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214779/suppl/GSM214779.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214780 | GPL570 |
|
24h DC5 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 95 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214780
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214780/suppl/GSM214780.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214781 | GPL570 |
|
24h DC5 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 31 yr; weight: 95 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214781
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214781/suppl/GSM214781.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214782 | GPL570 |
|
24h DC6
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 52 yr; weight: 75 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214782
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214782/suppl/GSM214782.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214783 | GPL570 |
|
24h DC6 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 52 yr; weight: 75 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214783
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214783/suppl/GSM214783.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214784 | GPL570 |
|
24h DC6 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 52 yr; weight: 75 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214784
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214784/suppl/GSM214784.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214785 | GPL570 |
|
24h DC IR
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214785
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214785/suppl/GSM214785.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214786 | GPL570 |
|
24h DC IR RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214786
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214786/suppl/GSM214786.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214787 | GPL570 |
|
24h DC C114R
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 41 yr; BMI: 30
Further information: Agostini at al. 2006 Cell Metabolism, 4 303-311
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214787
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214787/suppl/GSM214787.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214788 | GPL570 |
|
24h DC C114R RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 41 yr; BMI: 30
Further information: Agostini at al. 2006 Cell Metabolism, 4 303-311
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214788
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214788/suppl/GSM214788.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214789 | GPL570 |
|
24h DC C131Y
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 42 yr; BMI: 24.2
Further information: Agostini at al. 2006 Cell Metabolism, 4 303-311
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214789
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214789/suppl/GSM214789.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214790 | GPL570 |
|
24h DC C131Y RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 42 yr; BMI: 24.2
Further information: Agostini at al. 2006 Cell Metabolism, 4 303-311
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM214790
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214790/suppl/GSM214790.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214791 | GPL570 |
|
5d DC1
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 19 yr; weight: 65 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214791
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Bank
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214791/suppl/GSM214791.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214792 | GPL570 |
|
5d DC1 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 19 yr; weight: 65 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214792
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214792/suppl/GSM214792.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214793 | GPL570 |
|
5d DC2
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 47 yr; weight: 80 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214793
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214793/suppl/GSM214793.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214794 | GPL570 |
|
5d DC2 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 47 yr; weight: 80 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214794
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214794/suppl/GSM214794.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214795 | GPL570 |
|
5d DC3
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214795
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214795/suppl/GSM214795.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214796 | GPL570 |
|
5d DC3 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214796
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214796/suppl/GSM214796.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214797 | GPL570 |
|
5d DC4
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 21 yr; weight: 110 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214797
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214797/suppl/GSM214797.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214799 | GPL570 |
|
5d DC4 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 21 yr; weight: 110 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214799
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214799/suppl/GSM214799.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214800 | GPL570 |
|
5d DC4 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 21 yr; weight: 110 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214800
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214800/suppl/GSM214800.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214801 | GPL570 |
|
5d DC5
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 82 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214801
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214801/suppl/GSM214801.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214802 | GPL570 |
|
5d DC5 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 82 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214802
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214802/suppl/GSM214802.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214803 | GPL570 |
|
5d DC5 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 82 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214803
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214803/suppl/GSM214803.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214804 | GPL570 |
|
5d DC6
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 73 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214804
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214804/suppl/GSM214804.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214805 | GPL570 |
|
5d DC6 HS
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 73 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214805
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214805/suppl/GSM214805.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
GSM214806 | GPL570 |
|
5d DC6 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 73 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM214806
| | Sample_status | Public on Aug 20 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Blood Center
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | GC-RMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214806/suppl/GSM214806.CEL.gz
| | Sample_series_id | GSE8658
| | Sample_data_row_count | 54675
| | |
|
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