Search results for the GEO ID: GSE8665  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM214917 | GPL570 |
|
UET-13TR-EWS/FLI1 0hr
|
UET-13TR-EWS/FLI1 0hr
|
UET-13TR-EWS/FLI1 cells were cultured in the absence of tetracycline for 0 hours
|
Gene expression data from UET-13TR-EWS/FLI1 cells incubated in the absence of tetracycline for 0 hours
|
| Sample_geo_accession | GSM214917
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214917/suppl/GSM214917.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214917/suppl/GSM214917.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214917/suppl/GSM214917.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214918 | GPL570 |
|
UET-13TR-EWS/FLI1 24hr
|
UET-13TR-EWS/FLI1 24hr
|
UET-13TR-EWS/FLI1 cells were cultured in the absence of tetracycline for 24 hours
|
Gene expression data from UET-13TR-EWS/FLI1 cells incubated in the absence of tetracycline for 24 hours
|
| Sample_geo_accession | GSM214918
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214918/suppl/GSM214918.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214918/suppl/GSM214918.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214918/suppl/GSM214918.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214919 | GPL570 |
|
UET-13TR-EWS/FLI1 48hr
|
UET-13TR-EWS/FLI1 48hr
|
UET-13TR-EWS/FLI1 cells were cultured in the absence of tetracycline for 48 hours
|
Gene expression data from UET-13TR-EWS/FLI1 cells incubated in the absence of tetracycline for 48 hours
|
| Sample_geo_accession | GSM214919
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214919/suppl/GSM214919.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214919/suppl/GSM214919.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214919/suppl/GSM214919.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214920 | GPL570 |
|
UET-13TR-EWS/FLI1 72hr
|
UET-13TR-EWS/FLI1 72hr
|
UET-13TR-EWS/FLI1 cells were cultured in the absence of tetracycline for 72 hours
|
Gene expression data from UET-13TR-EWS/FLI1 cells incubated in the absence of tetracycline for 72 hours
|
| Sample_geo_accession | GSM214920
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214920/suppl/GSM214920.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214920/suppl/GSM214920.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214920/suppl/GSM214920.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214921 | GPL570 |
|
UET-13TR-EWS/FLI1 24hr tet+
|
UET-13TR-EWS/FLI1 24hr tet+
|
UET-13TR-EWS/FLI1 cells were cultured in the presence of tetracycline for 24 hours
|
Gene expression data from UET-13TR-EWS/FLI1 cells incubated in the presence of tetracycline for 24 hours
|
| Sample_geo_accession | GSM214921
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214921/suppl/GSM214921.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214921/suppl/GSM214921.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214921/suppl/GSM214921.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214922 | GPL570 |
|
UET-13TR-EWS/FLI1 48hr tet+
|
UET-13TR-EWS/FLI1 48hr tet+
|
UET-13TR-EWS/FLI1 cells were cultured in the presence of tetracycline for 48 hours
|
Gene expression data from UET-13TR-EWS/FLI1 cells incubated in the presence of tetracycline for 48 hours
|
| Sample_geo_accession | GSM214922
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214922/suppl/GSM214922.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214922/suppl/GSM214922.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214922/suppl/GSM214922.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214923 | GPL570 |
|
UET-13TR-EWS/FLI1 72hr tet+
|
UET-13TR-EWS/FLI1 72hr tet+
|
UET-13TR-EWS/FLI1 cells were cultured in the presence of tetracycline for 72 hours
|
Gene expression data from UET-13TR-EWS/FLI1 cells incubated in the presence of tetracycline for 72 hours
|
| Sample_geo_accession | GSM214923
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214923/suppl/GSM214923.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214923/suppl/GSM214923.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214923/suppl/GSM214923.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214924 | GPL570 |
|
UET-13TR-EWS/ERG 0hr
|
UET-13TR-EWS/ERG 0hr
|
UET-13TR-EWS/ERG cells were cultured in the absence of tetracycline for 0 hours
|
Gene expression data from UET-13TR-EWS/ERG cells incubated in the absence of tetracycline for 0 hours
|
| Sample_geo_accession | GSM214924
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214924/suppl/GSM214924.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214924/suppl/GSM214924.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214924/suppl/GSM214924.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214925 | GPL570 |
|
UET-13TR-EWS/ERG 24hr
|
UET-13TR-EWS/ERG 24hr
|
UET-13TR-EWS/ERG cells were cultured in the absence of tetracycline for 24 hours
|
Gene expression data from UET-13TR-EWS/ERG cells incubated in the absence of tetracycline for 24 hours
|
| Sample_geo_accession | GSM214925
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214925/suppl/GSM214925.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214925/suppl/GSM214925.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214925/suppl/GSM214925.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214926 | GPL570 |
|
UET-13TR-EWS/ERG 48hr
|
UET-13TR-EWS/ERG 48hr
|
UET-13TR-EWS/ERG cells were cultured in the absence of tetracycline for 48 hours
|
Gene expression data from UET-13TR-EWS/ERG cells incubated in the absence of tetracycline for 48 hours
|
| Sample_geo_accession | GSM214926
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214926/suppl/GSM214926.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214926/suppl/GSM214926.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214926/suppl/GSM214926.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214927 | GPL570 |
|
UET-13TR-EWS/ERG 72hr
|
UET-13TR-EWS/ERG 72hr
|
UET-13TR-EWS/ERG cells were cultured in the absence of tetracycline for 72 hours
|
Gene expression data from UET-13TR-EWS/ERG cells incubated in the absence of tetracycline for 72 hours
|
| Sample_geo_accession | GSM214927
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214927/suppl/GSM214927.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214927/suppl/GSM214927.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214927/suppl/GSM214927.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214928 | GPL570 |
|
UET-13TR-EWS/ERG 24hr tet+
|
UET-13TR-EWS/ERG 24hr tet+
|
UET-13TR-EWS/ERG cells were cultured in the presence of tetracycline for 24 hours
|
Gene expression data from UET-13TR-EWS/ERG cells incubated in the presence of tetracycline for 24 hours
|
| Sample_geo_accession | GSM214928
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214928/suppl/GSM214928.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214928/suppl/GSM214928.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214928/suppl/GSM214928.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214929 | GPL570 |
|
UET-13TR-EWS/ERG 48hr tet+
|
UET-13TR-EWS/ERG 48hr tet+
|
UET-13TR-EWS/ERG cells were cultured in the presence of tetracycline for 48 hours
|
Gene expression data from UET-13TR-EWS/ERG cells incubated in the presence of tetracycline for 48 hours
|
| Sample_geo_accession | GSM214929
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214929/suppl/GSM214929.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214929/suppl/GSM214929.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214929/suppl/GSM214929.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
|
GSM214930 | GPL570 |
|
UET-13TR-EWS/ERG72hr tet+
|
UET-13TR-EWS/ERG72hr tet+
|
UET-13TR-EWS/ERG cells were cultured in the presence of tetracycline for 72 hours
|
Gene expression data from UET-13TR-EWS/ERG cells incubated in the presence of tetracycline for 72 hours
|
| Sample_geo_accession | GSM214930
| | Sample_status | Public on Aug 03 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for the indicated periods.
| | Sample_growth_protocol_ch1 | UET-13 transfectants (UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG ) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% Tet System Approved Fetal Bovine Serum (T-FBS)(Takara, Shiga, Japan) at 37°C under a humidified 5% CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner GCS3000.
| | Sample_data_processing | The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshitaka,,Miyagawa
| | Sample_contact_email | miyayoshi0007@yahoo.co.jp
| | Sample_contact_institute | National Research Institute for Child Health and Development
| | Sample_contact_address | Setagaya-ku
| | Sample_contact_city | Okura
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214930/suppl/GSM214930.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214930/suppl/GSM214930.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214930/suppl/GSM214930.EXP.gz
| | Sample_series_id | GSE8665
| | Sample_data_row_count | 54675
| | |
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