Search results for the GEO ID: GSE8668  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM214982 | GPL570 |
|
Neutrophils before exercise 01
|
Human netrophils before exercise
|
neutrophil, healthy male, age 24
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214982
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214982/suppl/GSM214982.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214983 | GPL570 |
|
Neutrophils after exercise 01
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 24
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214983
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214983/suppl/GSM214983.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214984 | GPL570 |
|
Neutrophils before exercise 02
|
Human netrophils before exercise
|
neutrophil, healthy male, age 23
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214984
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214984/suppl/GSM214984.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214985 | GPL570 |
|
Neutrophils after exercise 02
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 23
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214985
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214985/suppl/GSM214985.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214986 | GPL570 |
|
Neutrophils before exercise 03
|
Human netrophils before exercise
|
neutrophil, healthy male, age 26
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214986
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214986/suppl/GSM214986.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214987 | GPL570 |
|
Neutrophils after exercise 03
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 26
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214987
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214987/suppl/GSM214987.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214988 | GPL570 |
|
Neutrophils before exercise 04
|
Human netrophils before exercise
|
neutrophil, healthy male, age 22
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214988
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214988/suppl/GSM214988.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214989 | GPL570 |
|
Neutrophils after exercise 04
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 22
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214989
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214989/suppl/GSM214989.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214990 | GPL570 |
|
Neutrophils before exercise 05
|
Human netrophils before exercise
|
neutrophil, healthy male, age 19
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214990
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214990/suppl/GSM214990.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214991 | GPL570 |
|
Neutrophils after exercise 05
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 19
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214991
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214991/suppl/GSM214991.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214992 | GPL570 |
|
Neutrophils before exercise 06
|
Human netrophils before exercise
|
neutrophil, healthy male, age 19
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214992
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214992/suppl/GSM214992.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214993 | GPL570 |
|
Neutrophils after exercise 06
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 19
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214993
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214993/suppl/GSM214993.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214994 | GPL570 |
|
Neutrophils before exercise 07
|
Human netrophils before exercise
|
neutrophil, healthy male, age 27
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214994
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214994/suppl/GSM214994.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214995 | GPL570 |
|
Neutrophils after exercise 07
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 27
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214995
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214995/suppl/GSM214995.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214996 | GPL570 |
|
Neutrophils before exercise 08
|
Human netrophils before exercise
|
neutrophil, healthy male, age 22
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214996
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214996/suppl/GSM214996.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214997 | GPL570 |
|
Neutrophils after exercise 08
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 22
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214997
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214997/suppl/GSM214997.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214998 | GPL570 |
|
Neutrophils before exercise 09
|
Human netrophils before exercise
|
neutrophil, healthy male, age 20
before-exercise
|
no additional
|
| Sample_geo_accession | GSM214998
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214998/suppl/GSM214998.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM214999 | GPL570 |
|
Neutrophils after exercise 09
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 20
after-exercise
|
no additional
|
| Sample_geo_accession | GSM214999
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM214nnn/GSM214999/suppl/GSM214999.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM215000 | GPL570 |
|
Neutrophils before exercise 10
|
Human netrophils before exercise
|
neutrophil, healthy male, age 19
before-exercise
|
no additional
|
| Sample_geo_accession | GSM215000
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215000/suppl/GSM215000.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM215001 | GPL570 |
|
Neutrophils after exercise 10
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 19
after-exercise
|
no additional
|
| Sample_geo_accession | GSM215001
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215001/suppl/GSM215001.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM215002 | GPL570 |
|
Neutrophils before exercise 11
|
Human netrophils before exercise
|
neutrophil, healthy male, age 29
before-exercise
|
no additional
|
| Sample_geo_accession | GSM215002
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215002/suppl/GSM215002.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM215003 | GPL570 |
|
Neutrophils after exercise 11
|
Human netrophils after 30 min bout of exercise
|
neutrophil, healthy male, age 29
after-exercise
|
no additional
|
| Sample_geo_accession | GSM215003
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215003/suppl/GSM215003.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
GSM215004 | GPL570 |
|
Neutrophils before exercise 12
|
Human netrophils before exercise
|
neutrophil, healthy male, age 24
before-exercise
|
no additional
|
| Sample_geo_accession | GSM215004
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Aug 02 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Neutrophils were isolated using OptiPrepÒ Density Gradient Medium (SIGMA). Using hematoxylin staining, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssistÒ version 4.0.3 (STRATAGENEÒ ). We normalized the data using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215004/suppl/GSM215004.CEL.gz
| | Sample_series_id | GSE8668
| | Sample_data_row_count | 54675
| | |
|
| |
| |
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