Search results for the GEO ID: GSE8687  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM215361 | GPL570 |
|
Sez-4 cell line starved of IL-2 biological rep1
|
Sez-4 cell line starved of IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215361
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215361/suppl/GSM215361.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215361/suppl/GSM215361.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215362 | GPL570 |
|
Sez-4 cell line starved of IL-2 biological rep2
|
Sez-4 cell line starved of IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215362
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215362/suppl/GSM215362.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215362/suppl/GSM215362.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215363 | GPL570 |
|
Sez-4 cell line starved of IL-2 biological rep3
|
Sez-4 cell line starved of IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215363
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215363/suppl/GSM215363.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215363/suppl/GSM215363.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215364 | GPL570 |
|
Sez-4 cell line starved of IL-2 and activated with IL-2 biological rep1
|
Sez-4 cell line starved of IL-2 and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215364
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215364/suppl/GSM215364.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215364/suppl/GSM215364.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215365 | GPL570 |
|
Sez-4 cell line starved of IL-2 and activated with IL-2 biological rep2
|
Sez-4 cell line starved of IL-2 and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215365
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215365/suppl/GSM215365.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215365/suppl/GSM215365.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215366 | GPL570 |
|
Sez-4 cell line starved of IL-2 and activated with IL-2 biological rep3
|
Sez-4 cell line starved of IL-2 and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215366
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215366/suppl/GSM215366.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215366/suppl/GSM215366.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215367 | GPL570 |
|
Sez-4 cell line starved of IL-2, pre-treated with pan-Jak inhibitor and activated with IL-2 biological rep1
|
Sez-4 cell line starved of IL-2, pre-treated with pan-Jak inhibitor and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215367
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215367/suppl/GSM215367.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215367/suppl/GSM215367.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215368 | GPL570 |
|
Sez-4 cell line starved of IL-2, pre-treated with pan-Jak inhibitor and activated with IL-2 biological rep2
|
Sez-4 cell line starved of IL-2, pre-treated with pan-Jak inhibitor and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215368
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215368/suppl/GSM215368.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215368/suppl/GSM215368.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215369 | GPL570 |
|
Sez-4 cell line starved of IL-2, pre-treated with pan-Jak inhibitor and activated with IL-2 biological rep3
|
Sez-4 cell line starved of IL-2, pre-treated with pan-Jak inhibitor and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215369
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215369/suppl/GSM215369.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215369/suppl/GSM215369.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215370 | GPL570 |
|
Sez-4 cell line starved of IL-2, pre-treated with Jak3 inhibitor and activated with IL-2 biological rep1
|
Sez-4 cell line starved of IL-2, pre-treated with Jak3 inhibitor and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215370
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215370/suppl/GSM215370.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215370/suppl/GSM215370.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215371 | GPL570 |
|
Sez-4 cell line starved of IL-2, pre-treated with Jak3 inhibitor and activated with IL-2 biological rep2
|
Sez-4 cell line starved of IL-2, pre-treated with Jak3 inhibitor and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215371
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215371/suppl/GSM215371.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215371/suppl/GSM215371.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
|
GSM215372 | GPL570 |
|
Sez-4 cell line starved of IL-2, pre-treated with Jak3 inhibitor and activated with IL-2 biological rep3
|
Sez-4 cell line starved of IL-2, pre-treated with Jak3 inhibitor and activated with IL-2
|
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
Gene expression profiling of Sez-4 cell line
|
| Sample_geo_accession | GSM215372
| | Sample_status | Public on Feb 19 2008
| | Sample_submission_date | Aug 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Sez-4 cell line was starved of IL-2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2h followed by pre-treating for 30’ with pan-Jak inhibitor (300 nM), Jak3 inhibitor (300 nM), or drig solvent and cultured with IL-2 (200U) or medium alone for 4 h.
| | Sample_growth_protocol_ch1 | Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
| | Sample_hyb_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_scan_protocol | according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
| | Sample_data_processing | CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariusz,,Wasik
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 3620 Hamilton Walk
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215372/suppl/GSM215372.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215372/suppl/GSM215372.EXP.gz
| | Sample_series_id | GSE8687
| | Sample_data_row_count | 54675
| | |
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