Search results for the GEO ID: GSE8692 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM215418 | GPL96 |
|
GBM/gliosarcoma_KH_24016-24007
|
tumor tissue GBM/gliosarcoma, patient tag KH, experiment 24016-24007
|
Tumor tissue: high grade glioblastoma/gliosarcoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215418
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215418/suppl/GSM215418.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215419 | GPL96 |
|
oligodendroglioma_IC_26342-26338
|
tumor tissue oligodendroglioma, patient tag IC, experiment 26342-26338
|
Tumor tissue: low grade oligodendroglioma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215419
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215419/suppl/GSM215419.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215420 | GPL96 |
|
GBM_1745_6948-6920
|
tumro tissue GBM, patient tag 1745, experiment 6948-6920
|
Tumor tissue: high grade glioblastoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215420
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215420/suppl/GSM215420.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215421 | GPL96 |
|
AMG_1649_34707-34697
|
tumor tissue AMG, patient tag 1649, experiment 34707-34697
|
Tumor tissue: low grade anaplastic mixed glioma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215421
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215421/suppl/GSM215421.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215422 | GPL96 |
|
GBM_1507_34705-34696
|
tumor tissue GBM, patient tag 1507, experiment 34705-34696
|
Tumor tissue: high grade glioblastoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215422
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215422/suppl/GSM215422.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215423 | GPL96 |
|
GBM_1354_4782-4767
|
tumor tissue GBM, patient tag 1354, experiment 4782-4767
|
Tumor tissue: high grade glioblastoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215423
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215423/suppl/GSM215423.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215424 | GPL96 |
|
AMG_1326_34703-34695
|
tumor tissue AMG, patient tag 1326, experiment 34704-34695
|
Tumor tissue: low grade anaplastic mixed glioma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215424
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215424/suppl/GSM215424.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215425 | GPL96 |
|
GBM_1043_14629-14597
|
tumor tissue GBM, patient tag 1043, experiment 14629-14597
|
Tumor tissue: high grade glioblastoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215425
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215425/suppl/GSM215425.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215426 | GPL96 |
|
GBM_932_15432-15410
|
tumor tissue GBM, patient tag 932, experiment 15432-15410
|
Tumor tissue: high grade glioblastoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215426
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215426/suppl/GSM215426.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215427 | GPL96 |
|
GBM_931_4776-4764
|
tumor tissue GBM, patient tag 931, experiment 4776-4764
|
Tumor tissue: high grade glioblastoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215427
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215427/suppl/GSM215427.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215428 | GPL96 |
|
gliosarcoma_762_26300-26291
|
tumor tissue gliosarcoma, patient tag 762, experiment 26300-26291
|
Tumor tissue: high grade gliosarcoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215428
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215428/suppl/GSM215428.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
GSM215429 | GPL96 |
|
gliosarcoma_631_6958-6955
|
tumor tissue gliosarcoma, patient tag 631, experiment 6958-6955
|
Tumor tissue: high grade gliosarcoma
|
All RNA samples had a 28S/18S ratio over 1.5 with no evidence of degradation. All scanned images were visually inspected for surface defects. Quality Control assessment of array data was performed using GCOS v1.4 (Affymetrix, Santa Clara, Ca) and Array Assist v5.0 build 30237 (Stratagene, La Jolla, Ca). Hybridization and Poly-A controls were within expected parameters, and 3'/5' ratios for actin and GAPDH were below 3 (mean Beta Actin 3'/5' = 1.53 and mean GAPDH 3'/5' = 1.02). GCOS v1.4 Expression Reports also revealed expected Call percentages (Present: 48% to 62%; Absent: 36% to 49%; Marginal: 1.5% to 1.9%). All arrays were within 1.5 fold of each other in overall intensity.
|
Sample_geo_accession | GSM215429
| Sample_status | Public on Aug 07 2007
| Sample_submission_date | Aug 03 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated from tumor samples using a TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) and was followed by a cleanup on a RNeasy column (Qiagen, Hilden, Germany).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cRNA probes were generated using standard Affymetrix protocols.
| Sample_hyb_protocol | Aliquots of each sample were hybridized to U133A oligonucleotide microarray using standard Affymetrix protocols.
| Sample_scan_protocol | The chips were scanned using the GeneArray scanner (Affymetrix) using standard Affymetrix protocols.
| Sample_data_processing | The CEL files generated by the Affymetrix Microarray Suite (MAS 5.0) were converted into DCP files using the DNA-Chip Analyzer (dCHIP 1.3).
| Sample_platform_id | GPL96
| Sample_contact_name | Tsunglin,,Liu
| Sample_contact_email | tliu@lifesci.ucsb.edu
| Sample_contact_institute | Neuroscience Research Institute
| Sample_contact_address | R5157, BIO II, UCSB
| Sample_contact_city | Santa Barbara
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 93106
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215429/suppl/GSM215429.CEL.gz
| Sample_series_id | GSE8692
| Sample_data_row_count | 22283
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|