Search results for the GEO ID: GSE8702  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM215632 | GPL570 |
|
LNCaP_Control_Time0
|
LNCaP_Control_Time0, LNCaP cells free of treatment at time0.
|
Cultured prostate cancer cells;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215632
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Control LNCaP Cells free of treatment.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Control cells were passaged approximately once per 10 days and plated at 1x106 cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215632/suppl/GSM215632.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215633 | GPL570 |
|
LNCaP_Control_3week
|
LNCaP_Control_3week, LNCaP cells free of treatment at 3week.
|
Cultured prostate cancer cells at 3 weeks;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215633
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Control LNCaP Cells free of treatment.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Control cells were passaged approximately once per 10 days and plated at 1x106 cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215633/suppl/GSM215633.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215634 | GPL570 |
|
LNCaP_AndrogenDeprived_3week_1
|
LNCaP_AndrogenDeprived_3week_1, LNCaP cells collected at androgen deprivation for 3 weeks.
|
Cultured prostate cancer cells deprived of androgen for 3 weeks;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215634
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 3 weeks.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215634/suppl/GSM215634.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215635 | GPL570 |
|
LNCaP_AndrogenDeprived_3week_2
|
LNCaP_AndrogenDeprived_3week_2, LNCaP cells collected at androgen deprivation for 3 weeks.
|
Cultured prostate cancer cells deprived of androgen for 3 weeks;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215635
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 3 weeks.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215635/suppl/GSM215635.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215636 | GPL570 |
|
LNCaP_Control_1month
|
LNCaP_Control_1month, LNCaP cells free of treatment at 1month
|
Cultured prostate cancer cells at one month;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215636
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Control LNCaP Cells free of treatment.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Control cells were passaged approximately once per 10 days and plated at 1x106 cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215636/suppl/GSM215636.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215637 | GPL570 |
|
LNCaP_AndrogenDeprived_1month_1
|
LNCaP_AndrogenDeprived_1month_1, LNCaP cells collected at androgen deprivation for 1 month.
|
Cultured prostate cancer cells deprived of androgen for 1 month;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215637
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 1 month.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215637/suppl/GSM215637.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215638 | GPL570 |
|
LNCaP_AndrogenDeprived_1month_2
|
LNCaP_AndrogenDeprived_1month_2, LNCaP cells collected at androgen deprivation for 1 month.
|
Cultured prostate cancer cells deprived of androgen for 1 month;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215638
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 1 month.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215638/suppl/GSM215638.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215639 | GPL570 |
|
LNCaP_Control_5month
|
LNCaP_Control_5month, LNCaP cells free of treatment at 5month
|
Cultured prostate cancer cells at 5 months;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215639
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Control LNCaP Cells free of treatment.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Control cells were passaged approximately once per 10 days and plated at 1x106 cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215639/suppl/GSM215639.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215640 | GPL570 |
|
LNCaP_AndrogenDeprived_5month_1
|
LNCaP_AndrogenDeprived_5month_1, LNCaP cells collected at androgen deprivation for 5 months.
|
Cultured prostate cancer cells deprived of androgen for 5 months;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215640
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 5 months.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215640/suppl/GSM215640.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215641 | GPL570 |
|
LNCaP_AndrogenDeprived_5month_2
|
LNCaP_AndrogenDeprived_5month_2, LNCaP cells collected at androgen deprivation for 5 months.
|
Cultured prostate cancer cells deprived of androgen for 5 months;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215641
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 5 months.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215641/suppl/GSM215641.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215642 | GPL570 |
|
LNCaP_Control_12month
|
LNCaP_Control_12month, LNCaP cells free of treatment at 12month
|
Cultured prostate cancer cells at 12 months;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215642
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Control LNCaP Cells free of treatment.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Control cells were passaged approximately once per 10 days and plated at 1x106 cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215642/suppl/GSM215642.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215643 | GPL570 |
|
LNCaP_AndrogenDeprived_12month_1
|
LNCaP_AndrogenDeprived_12month_1, LNCaP cells collected at androgen deprivation for 12 months.
|
Cultured prostate cancer cells deprived of androgen for 12 months;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215643
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 12 months.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215643/suppl/GSM215643.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215644 | GPL570 |
|
LNCaP_AndrogenDeprived_12month_2
|
LNCaP_AndrogenDeprived_12month_2, LNCaP cells collected at androgen deprivation for 12 months.
|
Cultured prostate cancer cells deprived of androgen for 12 months;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215644
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 12 months.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215644/suppl/GSM215644.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215645 | GPL570 |
|
LNCaP_AndrogenDeprived_11month_1
|
LNCaP_AndrogenDeprived_11month_1, LNCaP cells collected at androgen deprivation for 11 months.
|
Cultured prostate cancer cells deprived of androgen for 11 months;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215645
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 11 months.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215645/suppl/GSM215645.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215646 | GPL570 |
|
LNCaP_AndrogenDeprived_11month_2
|
LNCaP_AndrogenDeprived_11month_2, LNCaP cells collected at androgen deprivation for 11 months.
|
Cultured prostate cancer cells deprived of androgen for 11 months;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215646
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Under androgen deprivation treatment for 11 months.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215646/suppl/GSM215646.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
GSM215647 | GPL570 |
|
LNCaP_Control_CGF
|
LNCaP_Control_CGF, LNCaP cells free of treatment from CGF facility
|
Cultured prostate cancer cells from CGF facility;
|
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Sample_geo_accession | GSM215647
| | Sample_status | Public on Feb 29 2008
| | Sample_submission_date | Aug 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Control LNCaP Cells free of treatment.
| | Sample_growth_protocol_ch1 | LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Control cells were passaged approximately once per 10 days and plated at 1x106 cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen Rneasy Mini kit following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Beth,R.,Pflug
| | Sample_contact_email | pflugbr@upmc.edu
| | Sample_contact_phone | 412-623-3932
| | Sample_contact_laboratory | G-43
| | Sample_contact_department | Urology
| | Sample_contact_institute | University of Pittsburgh
| | Sample_contact_address | 5200 Centre Ave
| | Sample_contact_city | Pittsburgh
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 15232
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM215nnn/GSM215647/suppl/GSM215647.CEL.gz
| | Sample_series_id | GSE8702
| | Sample_data_row_count | 54613
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
