Search results for the GEO ID: GSE8840  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM219592 | GPL570 |
|
SCMC-RMZ,Alveolar,rhabdomyosarcoma cell line
|
SCMC-RMZ
|
Cell line from a rhabdomyosarcoma tumor
|
RMS cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219592
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | T. Sawada, Kyoto Prefectural University of Medicine, Japan
| | Sample_growth_protocol_ch1 | SCMC-RMZ cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219592/suppl/GSM219592.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219592/suppl/GSM219592.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219593 | GPL570 |
|
RMS,Alveolar,rhabdomyosarcoma cell line
|
RMS
|
Cell line from a rhabdomyosarcoma tumor
|
RMS cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219593
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Garvin AJ et al, Medical University of South Carolina, SC
| | Sample_growth_protocol_ch1 | RMS cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219593/suppl/GSM219593.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219593/suppl/GSM219593.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219726 | GPL570 |
|
RD,Embryonal,rhabdomyosarcoma cell line
|
RD
|
Cell line from a rhabdomyosarcoma tumor
|
RD cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219726
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC (CCL-136)
| | Sample_growth_protocol_ch1 | RD cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219726/suppl/GSM219726.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219726/suppl/GSM219726.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219743 | GPL570 |
|
RMS-YM,Embryonal,rhabdomyosarcoma cell line
|
RMS-YM
|
Cell line from a rhabdomyosarcoma tumor
|
RMS-YM cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219743
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | T. Naoe, Nagoya University School of Medicine, Japan
| | Sample_growth_protocol_ch1 | RMS-YM cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219743/suppl/GSM219743.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219743/suppl/GSM219743.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219745 | GPL570 |
|
RH18,Alveolar,rhabdomyosarcoma cell line
|
RH18
|
Cell line from a rhabdomyosarcoma tumor
|
RH18 cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219745
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | P. Houghton, St Jude Children's Research Hospital, Memphis, TN
| | Sample_growth_protocol_ch1 | RH18 cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219745/suppl/GSM219745.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219745/suppl/GSM219745.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219746 | GPL570 |
|
RUCH3,Embryonal,rhabdomyosarcoma cell line
|
RUCH3
|
Cell line from a rhabdomyosarcoma tumor
|
RUCH3 cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219746
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Beat Schäfer, University of Zürich, Switzerland
| | Sample_growth_protocol_ch1 | RUCH3 cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219746/suppl/GSM219746.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219746/suppl/GSM219746.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219747 | GPL570 |
|
CW9019,Alveolar,rhabdomyosarcoma cell line
|
CW9019
|
Cell line from a rhabdomyosarcoma tumor
|
CW9019 cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219747
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | JA. Biegel, University of Pennsylvania, Philadelphia, PA
| | Sample_growth_protocol_ch1 | CW9019 cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219747/suppl/GSM219747.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219747/suppl/GSM219747.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219748 | GPL570 |
|
RH41, Alveolar, rhabdomyosarcoma cell line
|
RH41
|
Cell line from a rhabdomyosarcoma tumor
|
RH41 cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219748
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | P. Houghton, St Jude Children's Research Hospital, Memphis, TN
| | Sample_growth_protocol_ch1 | RH41 cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219748/suppl/GSM219748.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219748/suppl/GSM219748.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219749 | GPL570 |
|
TE617T, rhabdomyosarcoma cell line
|
TE617T
|
Cell line from a rhabdomyosarcoma tumor
|
TE617T cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219749
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC (CRL-7774™)
| | Sample_growth_protocol_ch1 | TE617T cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219749/suppl/GSM219749.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219749/suppl/GSM219749.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219750 | GPL570 |
|
Hs729T,Pleomorphic,rhabdomyosarcoma cell line
|
Hs729T
|
Cell line from a rhabdomyosarcoma tumor
|
Hs729T cells were grown under standard conditions and harvested at 70%
confluency. Total RNA was isolated and analyzed on Affymetrix U133
Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219750
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 21 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC (CRL-7862™)
| | Sample_growth_protocol_ch1 | Hs729T cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer
| | Sample_label_protocol_ch1 | (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis
| | Sample_label_protocol_ch1 | and fragmented. Labeling was performed with One-Cycle cDNA synthesis
| | Sample_label_protocol_ch1 | Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was
| | Sample_label_protocol_ch1 | checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome
| | Sample_hyb_protocol | U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing | Expression data (.cel files) were normalized with the MAS5.0 algorithm
| | Sample_data_processing = (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219750/suppl/GSM219750.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219750/suppl/GSM219750.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219755 | GPL570 |
|
RUCH-2,Embryonal,rhabdomyosarcoma cell line
|
RUCH-2
|
Cell line from a rhabdomyosarcoma tumor
|
RMS cells were grown under standard conditions and harvested at 70% confluency. Total RNA was isolated and analyzed on Affymetrix U133 Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219755
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 22 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Beat Schäfer, University of Zürich, Switzerland
| | Sample_growth_protocol_ch1 | RUCH-2 cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing = Expression data (.cel files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219755/suppl/GSM219755.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219755/suppl/GSM219755.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219756 | GPL570 |
|
RH4,Alveolar,rhabdomyosarcoma cell line
|
RH4
|
Cell line from a rhabdomyosarcoma tumor
|
RH4 cells were grown under standard conditions and harvested at 70% confluency. Total RNA was isolated and analyzed on Affymetrix U133 Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219756
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 22 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | P. Houghton, St Jude Children’s Research Hospital , Memphis, TN
| | Sample_growth_protocol_ch1 | RH4 cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing = Expression data (.cel files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219756/suppl/GSM219756.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219756/suppl/GSM219756.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
| | |
|
GSM219757 | GPL570 |
|
RH30,Alveolar,rhabdomyosarcoma cell line
|
RH30
|
Cell line from a rhabdomyosarcoma tumor
|
RH30 cells were grown under standard conditions and harvested at 70% confluency. Total RNA was isolated and analyzed on Affymetrix U133 Plus 2.0 to generate expression profiles.
|
| Sample_geo_accession | GSM219757
| | Sample_status | Public on Jun 04 2009
| | Sample_submission_date | Aug 22 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | P. Houghton, St Jude Children's Research Hospital, Memphis, TNN
| | Sample_growth_protocol_ch1 | RH30 cells were cultured in RPMI (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
| | Sample_data_processing = Expression data (.cel files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219757/suppl/GSM219757.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM219nnn/GSM219757/suppl/GSM219757.CHP.gz
| | Sample_series_id | GSE8840
| | Sample_data_row_count | 54675
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