Search results for the GEO ID: GSE8946  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM226783 | GPL82 |
|
Hippocampi from saline treated control animal, biological rep. 1
|
Pooled hippocampi from 3 saline treated animals
|
Mixed background C57Bl6 and 129, Cre-, Creb1 flox/flox, Crem +/-
|
Gene expression data from control animals treated with saline
|
| Sample_geo_accession | GSM226783
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226783/suppl/GSM226783.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226784 | GPL82 |
|
Hippocampi from saline treated control animal, biological rep. 2
|
Pooled hippocampi from 3 saline treated animals
|
Mixed background C57Bl6 and 129, Cre-, Creb1 flox/flox, Crem +/-
|
Gene expression data from control animals treated with saline
|
| Sample_geo_accession | GSM226784
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226784/suppl/GSM226784.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226785 | GPL82 |
|
Hippocampi from saline treated control animal, biological rep. 3
|
Pooled hippocampi from 3 saline treated animals
|
Mixed background C57Bl6 and 129, Cre-, Creb1 flox/flox, Crem +/-
|
Gene expression data from control animals treated with saline
|
| Sample_geo_accession | GSM226785
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226785/suppl/GSM226785.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226786 | GPL82 |
|
Hippocampi from kainate treated control animal, biological rep. 1
|
Pooled hippocampi from 3 kainate treated animals
|
Mixed background C57Bl6 and 129, Cre-, Creb1 flox/flox, Crem +/-
|
Gene expression data from control animals treated with kainate
|
| Sample_geo_accession | GSM226786
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226786/suppl/GSM226786.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226787 | GPL82 |
|
Hippocampi from kainate treated control animal, biological rep. 2
|
Pooled hippocampi from 3 kainate treated animals
|
Mixed background C57Bl6 and 129, Cre-, Creb1 flox/flox, Crem +/-
|
Gene expression data from control animals treated with kainate
|
| Sample_geo_accession | GSM226787
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226787/suppl/GSM226787.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226788 | GPL82 |
|
Hippocampi from kainate treated control animal, biological rep. 3
|
Pooled hippocampi from 3 kainate treated animals
|
Mixed background C57Bl6 and 129, Cre-, Creb1 flox/flox, Crem +/-
|
Gene expression data from control animals treated with kainate
|
| Sample_geo_accession | GSM226788
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226788/suppl/GSM226788.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226789 | GPL82 |
|
Hippocampi from saline treated mutant animal, biological rep. 1
|
Pooled hippocampi from 3 saline treated mutant animals
|
Mixed background C57Bl6 and 129, Cre+, Creb1 flox/flox, Crem -/-
|
Gene expression data from mutant animals treated with saline
|
| Sample_geo_accession | GSM226789
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226789/suppl/GSM226789.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226790 | GPL82 |
|
Hippocampi from saline treated mutant animal, biological rep. 2
|
Pooled hippocampi from 3 saline treated mutant animals
|
Mixed background C57Bl6 and 129, Cre+, Creb1 flox/flox, Crem -/-
|
Gene expression data from mutant animals treated with saline
|
| Sample_geo_accession | GSM226790
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226790/suppl/GSM226790.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226791 | GPL82 |
|
Hippocampi from saline treated mutant animal, biological rep. 3
|
Pooled hippocampi from 3 saline treated mutant animals
|
Mixed background C57Bl6 and 129, Cre+, Creb1 flox/flox, Crem -/-
|
Gene expression data from mutant animals treated with saline
|
| Sample_geo_accession | GSM226791
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226791/suppl/GSM226791.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226792 | GPL82 |
|
Hippocampi from kainate treated mutant animal, biological rep. 1
|
Pooled hippocampi from 3 kainate treated mutant animals
|
Mixed background C57Bl6 and 129, Cre+, Creb1 flox/flox, Crem -/-
|
Gene expression data from mutant animals treated with kainate
|
| Sample_geo_accession | GSM226792
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226792/suppl/GSM226792.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226793 | GPL82 |
|
Hippocampi from kainate treated mutant animal, biological rep. 2
|
Pooled hippocampi from 3 kainate treated mutant animals
|
Mixed background C57Bl6 and 129, Cre+, Creb1 flox/flox, Crem -/-
|
Gene expression data from mutant animals treated with kainate
|
| Sample_geo_accession | GSM226793
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226793/suppl/GSM226793.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
| | |
|
GSM226794 | GPL82 |
|
Hippocampi from kainate treated mutant animal, biological rep. 3
|
Pooled hippocampi from 3 kainate treated mutant animals
|
Mixed background C57Bl6 and 129, Cre+, Creb1 flox/flox, Crem -/-
|
Gene expression data from mutant animals treated with kainate
|
| Sample_geo_accession | GSM226794
| | Sample_status | Public on Apr 03 2008
| | Sample_submission_date | Sep 04 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
| | Sample_growth_protocol_ch1 | 5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Bv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Jan,,Rodriguez Parkitna
| | Sample_contact_email | j.rodriguez@dkfz.de
| | Sample_contact_phone | +49 6221 423437
| | Sample_contact_laboratory | Molecular Biology of the Cell I
| | Sample_contact_institute | German Cancer Research Center
| | Sample_contact_address | Im Neuenheimer Feld 581
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM226nnn/GSM226794/suppl/GSM226794.CEL.gz
| | Sample_series_id | GSE8946
| | Sample_series_id | GSE8948
| | Sample_data_row_count | 12477
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