Search results for the GEO ID: GSE9117  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM230895 | GPL85 |
|
24h estrogen treatment
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myometrium
|
SD, female 70 days
|
none
|
| Sample_geo_accession | GSM230895
| | Sample_status | Public on Sep 21 2007
| | Sample_submission_date | Sep 20 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 60 days old rats were ovariectomized a week prior of treatment, estradiol benzoate in 10ug/kg sc was given (or vehicle for control animals), 6 or 24 h after treatment uterine horns were collected, myometrium was quickly separated and flash frozen in liquid nitrogen. Tissues collected from 6 animals were pooled fro further analysis
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | tissue was pulverized in liquid nitrogen and Trizol protocol was used for totalRNA extraction
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was prepared with Gibco BRL SuperScript Choice system, and biotinylated cRNA by in vitro transcription using Ambion MegaScript T7kit
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Genechip Rat Genome U34A array. GeneChips were washed at the Affymetrix Fluidics Station 400 and stained with SAPE.
| | Sample_scan_protocol | GeneChips were scanned using GeneArray2500 scanner
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Dora,,Agbas
| | Sample_contact_email | dagbas@kumc.edu
| | Sample_contact_laboratory | Smithlab
| | Sample_contact_department | Physiology
| | Sample_contact_institute | University of Kansas Medical Center
| | Sample_contact_address | 3901 Rainbow blvd.
| | Sample_contact_city | Kansas City
| | Sample_contact_state | KS
| | Sample_contact_zip/postal_code | 66160
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM230nnn/GSM230895/suppl/GSM230895.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM230nnn/GSM230895/suppl/GSM230895.EXP.gz
| | Sample_series_id | GSE9117
| | Sample_data_row_count | 8799
| | |
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GSM230896 | GPL85 |
|
24h control
|
myometrium
|
SD, female 70 days
|
none
|
| Sample_geo_accession | GSM230896
| | Sample_status | Public on Sep 21 2007
| | Sample_submission_date | Sep 20 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 60 days old rats were ovariectomized a week prior of treatment, estradiol benzoate in 10ug/kg sc was given (or vehicle for control animals), 6 or 24 h after treatment uterine horns were collected, myometrium was quickly separated and flash frozen in liquid nitrogen. Tissues collected from 6 animals were pooled fro further analysis
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | tissue was pulverized in liquid nitrogen and Trizol protocol was used for totalRNA extraction
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was prepared with Gibco BRL SuperScript Choice system, and biotinylated cRNA by in vitro transcription using Ambion MegaScript T7kit
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Genechip Rat Genome U34A array. GeneChips were washed at the Affymetrix Fluidics Station 400 and stained with SAPE.
| | Sample_scan_protocol | GeneChips were scanned using GeneArray2500 scanner
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Dora,,Agbas
| | Sample_contact_email | dagbas@kumc.edu
| | Sample_contact_laboratory | Smithlab
| | Sample_contact_department | Physiology
| | Sample_contact_institute | University of Kansas Medical Center
| | Sample_contact_address | 3901 Rainbow blvd.
| | Sample_contact_city | Kansas City
| | Sample_contact_state | KS
| | Sample_contact_zip/postal_code | 66160
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM230nnn/GSM230896/suppl/GSM230896.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM230nnn/GSM230896/suppl/GSM230896.EXP.gz
| | Sample_series_id | GSE9117
| | Sample_data_row_count | 8799
| | |
|
GSM230897 | GPL85 |
|
6h control
|
myometrium
|
SD, female 70 days
|
none
|
| Sample_geo_accession | GSM230897
| | Sample_status | Public on Sep 21 2007
| | Sample_submission_date | Sep 20 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 60 days old rats were ovariectomized a week prior of treatment, estradiol benzoate in 10ug/kg sc was given (or vehicle for control animals), 6 or 24 h after treatment uterine horns were collected, myometrium was quickly separated and flash frozen in liquid nitrogen. Tissues collected from 6 animals were pooled fro further analysis
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | tissue was pulverized in liquid nitrogen and Trizol protocol was used for totalRNA extraction
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was prepared with Gibco BRL SuperScript Choice system, and biotinylated cRNA by in vitro transcription using Ambion MegaScript T7kit
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Genechip Rat Genome U34A array. GeneChips were washed at the Affymetrix Fluidics Station 400 and stained with SAPE.
| | Sample_scan_protocol | GeneChips were scanned using GeneArray2500 scanner
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Dora,,Agbas
| | Sample_contact_email | dagbas@kumc.edu
| | Sample_contact_laboratory | Smithlab
| | Sample_contact_department | Physiology
| | Sample_contact_institute | University of Kansas Medical Center
| | Sample_contact_address | 3901 Rainbow blvd.
| | Sample_contact_city | Kansas City
| | Sample_contact_state | KS
| | Sample_contact_zip/postal_code | 66160
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM230nnn/GSM230897/suppl/GSM230897.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM230nnn/GSM230897/suppl/GSM230897.EXP.gz
| | Sample_series_id | GSE9117
| | Sample_data_row_count | 8799
| | |
|
GSM230898 | GPL85 |
|
6h estrogen treatment
|
myometrium
|
SD, female 70 days
|
none
|
| Sample_geo_accession | GSM230898
| | Sample_status | Public on Sep 21 2007
| | Sample_submission_date | Sep 20 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 60 days old rats were ovariectomized a week prior of treatment, estradiol benzoate in 10ug/kg sc was given (or vehicle for control animals), 6 or 24 h after treatment uterine horns were collected, myometrium was quickly separated and flash frozen in liquid nitrogen. Tissues collected from 6 animals were pooled fro further analysis
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | tissue was pulverized in liquid nitrogen and Trizol protocol was used for totalRNA extraction
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was prepared with Gibco BRL SuperScript Choice system, and biotinylated cRNA by in vitro transcription using Ambion MegaScript T7kit
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Genechip Rat Genome U34A array. GeneChips were washed at the Affymetrix Fluidics Station 400 and stained with SAPE.
| | Sample_scan_protocol | GeneChips were scanned using GeneArray2500 scanner
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Dora,,Agbas
| | Sample_contact_email | dagbas@kumc.edu
| | Sample_contact_laboratory | Smithlab
| | Sample_contact_department | Physiology
| | Sample_contact_institute | University of Kansas Medical Center
| | Sample_contact_address | 3901 Rainbow blvd.
| | Sample_contact_city | Kansas City
| | Sample_contact_state | KS
| | Sample_contact_zip/postal_code | 66160
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM230nnn/GSM230898/suppl/GSM230898.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM230nnn/GSM230898/suppl/GSM230898.EXP.gz
| | Sample_series_id | GSE9117
| | Sample_data_row_count | 8799
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