Search results for the GEO ID: GSE9150  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM231234 | GPL570 |
|
Allergic_saline_pt1
|
epithelial cells cultured from biobsy, saline exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231234
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231234/suppl/GSM231234.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231234/suppl/GSM231234.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231235 | GPL570 |
|
Allergic_HDM_pt1
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231235
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231235/suppl/GSM231235.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231235/suppl/GSM231235.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231236 | GPL570 |
|
Allergic_saline_pt2
|
epithelial cells cultured from biobsy, saline exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231236
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231236/suppl/GSM231236.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231236/suppl/GSM231236.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231237 | GPL570 |
|
Allergic_HDM_pt2
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231237
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231237/suppl/GSM231237.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231237/suppl/GSM231237.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231238 | GPL570 |
|
Allergic_saline_pt3
|
epithelial cells cultured from biobsy, saline exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231238
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231238/suppl/GSM231238.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231238/suppl/GSM231238.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231239 | GPL570 |
|
Allergic_HDM_pt3
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231239
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231239/suppl/GSM231239.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231239/suppl/GSM231239.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231240 | GPL570 |
|
Allergic_saline_pt4
|
epithelial cells cultured from biobsy, saline exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231240
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231240/suppl/GSM231240.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231240/suppl/GSM231240.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231241 | GPL570 |
|
Allergic_HDM_pt4
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231241
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231241/suppl/GSM231241.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231241/suppl/GSM231241.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231242 | GPL570 |
|
Allergic_saline_pt5
|
epithelial cells cultured from biobsy, saline exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231242
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231242/suppl/GSM231242.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231242/suppl/GSM231242.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231243 | GPL570 |
|
Allergic_HDM_pt5
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
monotypic nouse dust mite allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231243
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231243/suppl/GSM231243.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231243/suppl/GSM231243.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231244 | GPL570 |
|
control_saline_pt2
|
epithelial cells cultured from biobsy, saline exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231244
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231244/suppl/GSM231244.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231244/suppl/GSM231244.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231245 | GPL570 |
|
control_HDM_pt2
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231245
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231245/suppl/GSM231245.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231245/suppl/GSM231245.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231246 | GPL570 |
|
control_saline_pt3
|
epithelial cells cultured from biobsy, saline exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231246
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231246/suppl/GSM231246.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231246/suppl/GSM231246.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231247 | GPL570 |
|
control_HDM_pt3
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231247
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231247/suppl/GSM231247.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231247/suppl/GSM231247.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231248 | GPL570 |
|
control_saline_pt7
|
epithelial cells cultured from biobsy, saline exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231248
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231248/suppl/GSM231248.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231248/suppl/GSM231248.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231249 | GPL570 |
|
control_HDM_pt7
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231249
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231249/suppl/GSM231249.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231249/suppl/GSM231249.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231250 | GPL570 |
|
control_saline_pt8
|
epithelial cells cultured from biobsy, saline exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231250
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231250/suppl/GSM231250.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231250/suppl/GSM231250.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231251 | GPL570 |
|
control_HDM_pt8
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231251
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231251/suppl/GSM231251.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231251/suppl/GSM231251.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231252 | GPL570 |
|
control_saline_pt9
|
epithelial cells cultured from biobsy, saline exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231252
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231252/suppl/GSM231252.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231252/suppl/GSM231252.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
GSM231253 | GPL570 |
|
control_HDM_pt9
|
epithelial cells cultured from biobsy, house dust mite exposed 24h
|
non allergic
|
Gene expression data from cells after exposure to saline or HDM
|
| Sample_geo_accession | GSM231253
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Sep 24 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aram,Ben,Vroling
| | Sample_contact_email | aramvroling@hotmail.com
| | Sample_contact_laboratory | ENT
| | Sample_contact_department | ENT
| | Sample_contact_institute | AMC
| | Sample_contact_address | meibergdreef 9
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DD
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231253/suppl/GSM231253.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231253/suppl/GSM231253.EXP.gz
| | Sample_series_id | GSE9150
| | Sample_data_row_count | 54613
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
