Search results for the GEO ID: GSE9159 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM231385 | GPL570 |
|
Myometrium_term labor_Flap28
|
Myometrium_term labor_Flap28
|
Tissue: myometrium, Gender: female, Gestational age: 39 weeks, Condition: term labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231385
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231385/suppl/GSM231385.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231385/suppl/GSM231385.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231386 | GPL570 |
|
Myometrium_term labor_Flap29
|
Myometrium_term labor_Flap29
|
Tissue: myometrium, Gender: female, Gestational age: 39 weeks, Condition: term labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231386
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231386/suppl/GSM231386.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231386/suppl/GSM231386.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231387 | GPL570 |
|
Myometrium_term labor_Flap35
|
Myometrium_term labor_Flap35
|
Tissue: myometrium, Gender: female, Gestational age: 39 weeks, Condition: term labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231387
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231387/suppl/GSM231387.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231387/suppl/GSM231387.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231390 | GPL570 |
|
Myometrium_term no labor_Mem72
|
Myometrium_term no labor_Mem72
|
Tissue: myometrium, Gender: female, Gestational age: 39 weeks, Condition: term labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231390
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231390/suppl/GSM231390.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231390/suppl/GSM231390.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231391 | GPL570 |
|
Myometrium_term no labor_Prot12
|
Myometrium_term no labor_Prot12
|
Tissue: myometrium, Gender: female, Gestational age: 39 weeks, Condition: term labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231391
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231391/suppl/GSM231391.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231391/suppl/GSM231391.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231393 | GPL570 |
|
Myometrium_term no labor_T16
|
Myometrium_term no labor_T16
|
Tissue: myometrium, Gender: female, Gestational age: 39 weeks, Condition: term labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231393
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231393/suppl/GSM231393.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231393/suppl/GSM231393.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231396 | GPL570 |
|
Myometrium_preterm labor_N20
|
Myometrium_preterm labor_N20
|
Tissue: myometrium, Gender: female, Gestational age: 31 weeks, Condition: preterm labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231396
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231396/suppl/GSM231396.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231396/suppl/GSM231396.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231400 | GPL570 |
|
Myometrium_preterm labor_T84
|
Myometrium_preterm labor_T84
|
Tissue: myometrium, Gender: female, Gestational age: 31 weeks, Condition: preterm labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231400
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231400/suppl/GSM231400.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231400/suppl/GSM231400.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231404 | GPL570 |
|
Myometrium_preterm labor_T53
|
Myometrium_preterm labor_T53
|
Tissue: myometrium, Gender: female, Gestational age: 31 weeks, Condition: preterm labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231404
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231404/suppl/GSM231404.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231404/suppl/GSM231404.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231408 | GPL570 |
|
Myometrium_preterm no labor_T18
|
Myometrium_preterm no labor_T18
|
Tissue: myometrium, Gender: female, Gestational age: 29 weeks, Condition: preterm no labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231408
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231408/suppl/GSM231408.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231408/suppl/GSM231408.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231410 | GPL570 |
|
Myometrium_preterm no labor_T25
|
Myometrium_preterm no labor_T25
|
Tissue: myometrium, Gender: female, Gestational age: 29 weeks, Condition: preterm no labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231410
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231410/suppl/GSM231410.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231410/suppl/GSM231410.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
|
GSM231411 | GPL570 |
|
Myometrium_preterm no labor_T36
|
Myometrium_preterm no labor_T36
|
Tissue: myometrium, Gender: female, Gestational age: 29 weeks, Condition: preterm no labor
|
All microarray procedures were performed according to Affymetrix standard protocol (Affymetrix genechip expression analysis technical manual).
|
Sample_geo_accession | GSM231411
| Sample_status | Public on Sep 27 2008
| Sample_submission_date | Sep 25 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol™ Reagent (Invitrogen™, Carlsbad, CA) according to the manufacturer’s protocol. RNA qunatity and quality was reassessed prior to spotting using the Agilent 2100 Bioanalyzer™ (Agilent Technologies, Palo Alto, CA)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled cRNA was produce according to manufacturers standard procdures (Affymetrix genechip expression analysis technical manual). cDNA is synthesized from total RNA. An In vitro transcription reaction is then done to produce biotin-labeled cRNA from the cDNA. The cRNA is fragmented before hybridization.
| Sample_hyb_protocol | A hybridization cocktail is prepared, including the fragmented target cRNA, probe array controls, BSA, and herring sperm DNA. It is then hybridized to the probe array during a 16 hour incubation using Affymetrix hybridization oven 640. Immediately following hybridization, the probe array undergoes automated washing and staining protocol (Affymetrix genechip expression analysis technical manual) on the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Probe array was then scanned using the Affymetrix GeneChip Scanner 3000 according to standard protocol (Affymetrix genechip expression analysis technical manual).
| Sample_data_processing | Oligonucleotide microarrays were analyzed using the Affymetrix Expression Console™. Gene expression levels were normalized using the R Statistical package and software available through the Bioconductor Project. Normalization was carried out using a background adjustment procedure and a sequence-specific expression method (GC-RMA). A detection P value was used to make a reliable call of gene expression (present, marginal, or absent). Genes present in only one of the three patient samples were classified as absent overall.
| Sample_platform_id | GPL570
| Sample_contact_name | Carl,P,Weiner
| Sample_contact_institute | University of Kansas Medical Center
| Sample_contact_address | 3901 Rainbow Boulevard, MS 2028
| Sample_contact_city | Kansas City
| Sample_contact_state | KS
| Sample_contact_zip/postal_code | 66160-7316
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231411/suppl/GSM231411.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM231nnn/GSM231411/suppl/GSM231411.CHP.gz
| Sample_series_id | GSE9159
| Sample_data_row_count | 54675
| |
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