Search results for the GEO ID: GSE9264  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM235038 | GPL570 |
|
miR-155 r1
|
293 cells with pcDNA3.1/V5/HisA
|
human embryonic kidney cells expressing miR-155
|
gene expression from 293 cells with pcDNA3.1/V5/HisA
|
| Sample_geo_accession | GSM235038
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235038/suppl/GSM235038.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235038/suppl/GSM235038.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235039 | GPL570 |
|
miR-155 r2
|
293 cells with pcDNA3.1/V5/HisA
|
human embryonic kidney cells expressing miR-155
|
gene expression from 293 cells with pcDNA3.1/V5/HisA
|
| Sample_geo_accession | GSM235039
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235039/suppl/GSM235039.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235039/suppl/GSM235039.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235040 | GPL570 |
|
miR-155 r3
|
293 cells with pcDNA3.1/V5/HisA
|
human embryonic kidney cells expressing miR-155
|
gene expression from 293 cells with pcDNA3.1/V5/HisA
|
| Sample_geo_accession | GSM235040
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235040/suppl/GSM235040.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235040/suppl/GSM235040.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235041 | GPL570 |
|
miR-155 r4
|
293 cells with pcDNA3.1/V5/HisA
|
human embryonic kidney cells expressing miR-155
|
gene expression from 293 cells with pcDNA3.1/V5/HisA
|
| Sample_geo_accession | GSM235041
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235041/suppl/GSM235041.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235041/suppl/GSM235041.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235042 | GPL570 |
|
miR-K12-11 r1
|
293 cells with pmiRNA cluster
|
human embryonic kidney cells expressing miR-K12-11
|
gene expression from 293 cells with pmiRNA cluster
|
| Sample_geo_accession | GSM235042
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235042/suppl/GSM235042.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235042/suppl/GSM235042.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235043 | GPL570 |
|
miR-K12-11 r2
|
293 cells with pmiRNA cluster
|
human embryonic kidney cells expressing miR-K12-11
|
gene expression from 293 cells with pmiRNA cluster
|
| Sample_geo_accession | GSM235043
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235043/suppl/GSM235043.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235043/suppl/GSM235043.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235044 | GPL570 |
|
miR-K12-11 r3
|
293 cells with pmiRNA cluster
|
human embryonic kidney cells expressing miR-K12-11
|
gene expression from 293 cells with pmiRNA cluster
|
| Sample_geo_accession | GSM235044
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235044/suppl/GSM235044.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235044/suppl/GSM235044.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235045 | GPL570 |
|
miR-K12-11 r4
|
293 cells with pmiRNA cluster
|
human embryonic kidney cells expressing miR-K12-11
|
gene expression from 293 cells with pmiRNA cluster
|
| Sample_geo_accession | GSM235045
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235045/suppl/GSM235045.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235045/suppl/GSM235045.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235046 | GPL570 |
|
pcDNA3.1 r1
|
293 cells with pcDNA3.1/V5/HisA
|
human embryonic kidney cells expressing control vector pcDNA3.1/V5/HisA
|
gene expression from 293 cells with pmiRNA cluster
|
| Sample_geo_accession | GSM235046
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235046/suppl/GSM235046.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235046/suppl/GSM235046.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235047 | GPL570 |
|
pcDNA3.1 r2
|
293 cells with pcDNA3.1/V5/HisA
|
human embryonic kidney cells expressing control vector pcDNA3.1/V5/HisA
|
gene expression from 293 cells with pmiRNA cluster
|
| Sample_geo_accession | GSM235047
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235047/suppl/GSM235047.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235047/suppl/GSM235047.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235048 | GPL570 |
|
pcDNA3.1 r3
|
293 cells with pcDNA3.1/V5/HisA
|
human embryonic kidney cells expressing control vector pcDNA3.1/V5/HisA
|
gene expression from 293 cells with pmiRNA cluster
|
| Sample_geo_accession | GSM235048
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235048/suppl/GSM235048.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235048/suppl/GSM235048.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
|
GSM235049 | GPL570 |
|
pcDNA3.1 r4
|
293 cells with pcDNA3.1/V5/HisA
|
human embryonic kidney cells expressing control vector pcDNA3.1/V5/HisA
|
gene expression from 293 cells with pmiRNA cluster
|
| Sample_geo_accession | GSM235049
| | Sample_status | Public on Oct 09 2007
| | Sample_submission_date | Oct 09 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
| | Sample_growth_protocol_ch1 | grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
| | Sample_data_processing | The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
| | Sample_platform_id | GPL570
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235049/suppl/GSM235049.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM235nnn/GSM235049/suppl/GSM235049.EXP.gz
| | Sample_series_id | GSE9264
| | Sample_data_row_count | 54675
| | |
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