Search results for the GEO ID: GSE9294  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM236911 | GPL1261 |
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EOM side population 0Esp
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Side population cells of EOM
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C57BL/10ScSnJ mice were used to have SP cells of EOM muscle.
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236912 | GPL1261 |
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EOM side population 1Esp
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Side population cells of EOM
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C57BL/10ScSnJ mice were used to have SP cells of EOM muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236913 | GPL1261 |
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EOM side population 2Esp
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Side population cells of EOM
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C57BL/10ScSnJ mice were used to have SP cells of EOM muscle.
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236914 | GPL1261 |
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EOM side population 3Esp
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Side population cells of EOM
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C57BL/10ScSnJ mice were used to have SP cells of EOM muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236915 | GPL1261 |
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EOM side population 5Esp
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Side population cells of EOM
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C57BL/10ScSnJ mice were used to have SP cells of EOM muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236916 | GPL1261 |
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EOM side population 6Esp
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Side population cells of EOM
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C57BL/10ScSnJ mice were used to have SP cells of EOM muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236917 | GPL1261 |
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TA side population 0Tsp
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Side population cells of TA
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C57BL/10ScSnJ mice were used to have SP cells of TA muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236918 | GPL1261 |
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TA side population 1Tsp
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Side population cells of TA
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C57BL/10ScSnJ mice were used to have SP cells of TA muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236919 | GPL1261 |
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TA side population 2Tsp
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Side population cells of TA
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C57BL/10ScSnJ mice were used to have SP cells of TA muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236920 | GPL1261 |
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TA side population 3Tsp
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Side population cells of TA
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C57BL/10ScSnJ mice were used to have SP cells of TA muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236921 | GPL1261 |
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TA side population 5Tsp
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Side population cells of TA
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C57BL/10ScSnJ mice were used to have SP cells of TA muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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GSM236923 | GPL1261 |
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Ta side population 6Tsp
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Side population cells of TA
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C57BL/10ScSnJ mice were used to have SP cells of TA muscle
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SP cell preparations were washed, pelleted and resuspended in 20µl of PBS and stored at –80oC until processing for microarray analysis using manufacturers instructions and previously described methods from our laboratory . Total RNA was isolated using RNeasy micro kit (Qiagen, Valencia, CA). The purity, concentration and integrity of RNA was determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA). RNAs were subjected to linear amplification using the GeneChip® Two cycle target-labeling protocol (Affymetrix Inc., Santa Clara, CA, USA).
Six independently separated EOM and six TA SP cell preparations were used for microarray analysis using the Affymetrix® Mouse 430 ver 2.0 GeneChip arrays. Raw intensities for each probe set were stored in electronic formats by expression summaries calculated using Microarray Suite version 5.0 (MAS5) algorithms.
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