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Search results for the GEO ID: GSE9300

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM237013

GPL85

Ovary postnatal day 0 replicate 1

0-day rat ovary

ovaries dissected from 0-day old Sprague-Dawley rats and cleaned of ovarian bursa.

Chip ID=phil_P0_U34A_102502. RNA from zero-day old rat ovaries.

GSM237014

GPL85

Ovary postnatal day 0 replicate 2

0-day rat ovary

ovaries dissected from 0-day old Sprague-Dawley rats and cleaned of ovarian bursa.

Chip ID=Phil-PO-RGu34A-072403. RNA from zero-day old rat ovaries.

GSM237015

GPL85

Ovary postnatal day 4 replicate 1

P4-day rat ovary

ovaries dissected from P4-day old Sprague-Dawley rats and cleaned of ovarian bursa.

Chip ID=phil_P4_U34A_102502. RNA from four-day old rat ovaries.

GSM237016

GPL85

Ovary postnatal day 4 replicate 2

P4-day rat ovary

ovaries dissected from P4-day old Sprague-Dawley rats and cleaned of ovarian bursa.

Chip ID=Phil-P4-RGu34A-072403. RNA from four-day old rat ovaries.

GSM237017

GPL85

Cultured ovary postnatal day 0 replicate 1

0-day rat ovary cultured 7 days

ovaries dissected from 0-day old Sprague-Dawley rats and cleaned of ovarian bursa and cultured for 7 days

Chip ID=Phil-Cult.Ovary-RGu34A-082603. RNA from zero-day old rat ovaries cultured 7 days.

Sample_geo_accession	GSM237017
Sample_status	Public on Oct 12 2007
Sample_submission_date	Oct 11 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female day P0 rat pups were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ?g/ml transferrin, 200 ng/ml insulin (human recombinant, Sigma), and 0.05 mg/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	About 20 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
Sample_platform_id	GPL85
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM237nnn/GSM237017/suppl/GSM237017.CEL.gz
Sample_series_id	GSE9300
Sample_data_row_count	8799

GSM237018

GPL85

Cultured ovary postnatal day 0 replicate 2

0-day rat ovary cultured 7 days

ovaries dissected from 0-day old Sprague-Dawley rats and cleaned of ovarian bursaand cultured for 7 days

Chip ID=phil-cultured-U34A-022703. RNA from zero-day old rat ovaries cultured 7 days.

Sample_geo_accession	GSM237018
Sample_status	Public on Oct 12 2007
Sample_submission_date	Oct 11 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female day P0 rat pups were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ?g/ml transferrin, 200 ng/ml insulin (human recombinant, Sigma), and 0.05 mg/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	About 20 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
Sample_platform_id	GPL85
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM237nnn/GSM237018/suppl/GSM237018.CEL.gz
Sample_series_id	GSE9300
Sample_data_row_count	8799

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