Search results for the GEO ID: GSE9350  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM238018 | GPL570 |
|
FG cell line_hypoxic growth_replicate 1
|
Pancreatic cancer cell line FG
|
low metastatic potential; growth under reduced oxygen
|
none
|
| Sample_geo_accession | GSM238018
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238018/suppl/GSM238018.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238019 | GPL570 |
|
FG cell line_hypoxic growth_replicate 2
|
Pancreatic cancer cell line FG
|
low metastatic potential; growth under reduced oxygen
|
none
|
| Sample_geo_accession | GSM238019
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238019/suppl/GSM238019.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238020 | GPL570 |
|
FG cell line_hypoxic growth_replicate 3
|
Pancreatic cancer cell line FG
|
low metastatic potential; growth under reduced oxygen
|
none
|
| Sample_geo_accession | GSM238020
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238020/suppl/GSM238020.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238021 | GPL570 |
|
FG cell line_normoxic growth_replicate 1
|
Pancreatic cancer cell line FG
|
low metastatic potential; growth under normal oxygen
|
none
|
| Sample_geo_accession | GSM238021
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238021/suppl/GSM238021.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238022 | GPL570 |
|
FG cell line_normoxic growth_replicate 2
|
Pancreatic cancer cell line FG
|
low metastatic potential; growth under normal oxygen
|
none
|
| Sample_geo_accession | GSM238022
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238022/suppl/GSM238022.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238023 | GPL570 |
|
FG cell line_normoxic growth_replicate 3
|
Pancreatic cancer cell line FG
|
low metastatic potential; growth under normal oxygen
|
none
|
| Sample_geo_accession | GSM238023
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238023/suppl/GSM238023.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238024 | GPL570 |
|
L3.6pl cell line_hypoxic growth_replicate 1
|
Pancreatic cancer cell line L3.6pl
|
high metastatic potential; growth under reduced oxygen
|
none
|
| Sample_geo_accession | GSM238024
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238024/suppl/GSM238024.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238025 | GPL570 |
|
L3.6pl cell line_hypoxic growth_replicate 2
|
Pancreatic cancer cell line L3.6pl
|
high metastatic potential; growth under reduced oxygen
|
none
|
| Sample_geo_accession | GSM238025
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238025/suppl/GSM238025.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238026 | GPL570 |
|
L3.6pl cell line_hypoxic growth_replicate 3
|
Pancreatic cancer cell line L3.6pl
|
high metastatic potential; growth under reduced oxygen
|
none
|
| Sample_geo_accession | GSM238026
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238026/suppl/GSM238026.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238027 | GPL570 |
|
L3.6pl cell line_normoxic growth_replicate 1
|
Pancreatic cancer cell line L3.6pl
|
high metastatic potential; growth under normal oxygen
|
none
|
| Sample_geo_accession | GSM238027
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238027/suppl/GSM238027.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238028 | GPL570 |
|
L3.6pl cell line_normoxic growth_replicate 2
|
Pancreatic cancer cell line L3.6pl
|
high metastatic potential; growth under normal oxygen
|
none
|
| Sample_geo_accession | GSM238028
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238028/suppl/GSM238028.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
| | |
|
GSM238029 | GPL570 |
|
L3.6pl cell line_normoxic growth_replicate 3
|
Pancreatic cancer cell line L3.6pl
|
high metastatic potential; growth under normal oxygen
|
none
|
| Sample_geo_accession | GSM238029
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Oct 17 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_growth_protocol_ch1 | Two human pancreatic carcinoma cell lines: FG, L3.6pl (Bruns et al., 1999) were used in this study. Both cell lines were cultivated in Dulbecco's Modified Eagle Medium (Gibco/Invitrogen) supplemented with 10% foetal bovine serum (Biochrom, Lot No.: 923 FF), 1x Glutamax-1 (Gibco/Invitrogen), 2x MEM vitamin solution (PAN Biotech, Aidenbach Germany), 2x MEM NEAA (PAN Biotech, Aidenbach Germany), 1x Penicillin/streptomycin (100 U/ml Penicillin + 0,1 mg/ml streptomycin) (PAN Biotech, Aidenbach Germany). Cells were cultivated at 37°C and 5% CO2 starting from frozen stock without further passaging. 500000 frozen cells were plated into 175cm2 Nunclon Δ-treated flask with filter cap (Nunc Roskilde Denmark) containing 25 ml medium and cultivated until an apparent confluence of 80% was reached (typically 9 days). Medium was changed every second day. The last 24 hours cells were treated by hypoxia where air was replaced by nitrogen to reach an oxygen concentration of 1% or left untreated respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured cells with the Trizol method according to the manufacturers protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
| | Sample_hyb_protocol | fragmented cRNA was hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefan,,Krebs
| | Sample_contact_email | krebs-st@lmb.uni-muenchen.de
| | Sample_contact_phone | 0049-89-2180 76715
| | Sample_contact_laboratory | Lafuga Genomics
| | Sample_contact_department | Gene Center
| | Sample_contact_institute | Ludwig-Maximilian University
| | Sample_contact_address | Feodor-Lynen-Str. 25
| | Sample_contact_city | Munich
| | Sample_contact_zip/postal_code | 81377
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238029/suppl/GSM238029.CEL.gz
| | Sample_series_id | GSE9350
| | Sample_data_row_count | 54675
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