Search results for the GEO ID: GSE9354 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM238320 | GPL339 |
|
monolayer_3T3_EN_transduced, sample 1, replicate 1
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ETV6-NTRK3 transduced NIH 3T3 cells
|
3T3 cells growing as a monolayer
|
Gene expression data from EN-transduced 3T3 cells
|
Sample_geo_accession | GSM238320
| Sample_status | Public on Dec 14 2007
| Sample_submission_date | Oct 17 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted by using Trizol following the manufacturar's instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430A chips. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip.
| Sample_platform_id | GPL339
| Sample_contact_name | Zhe,,Li
| Sample_contact_email | li@bloodgroup.tch.harvard.edu
| Sample_contact_phone | 617-919-2052
| Sample_contact_laboratory | Stuart Orkin's Lab
| Sample_contact_department | Division of Hematology/Oncology
| Sample_contact_institute | Children's Hospital Boston
| Sample_contact_address |
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238320/suppl/GSM238320.CEL.gz
| Sample_series_id | GSE9354
| Sample_data_row_count | 22690
| |
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GSM238321 | GPL339 |
|
monolayer_3T3_EN_transduced, sample 1, replicate 2
|
ETV6-NTRK3 transduced NIH 3T3 cells
|
3T3 cells growing as a monolayer
|
Gene expression data from EN-transduced 3T3 cells
|
Sample_geo_accession | GSM238321
| Sample_status | Public on Dec 14 2007
| Sample_submission_date | Oct 17 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted by using Trizol following the manufacturar's instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430A chips. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip.
| Sample_platform_id | GPL339
| Sample_contact_name | Zhe,,Li
| Sample_contact_email | li@bloodgroup.tch.harvard.edu
| Sample_contact_phone | 617-919-2052
| Sample_contact_laboratory | Stuart Orkin's Lab
| Sample_contact_department | Division of Hematology/Oncology
| Sample_contact_institute | Children's Hospital Boston
| Sample_contact_address |
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238321/suppl/GSM238321.CEL.gz
| Sample_series_id | GSE9354
| Sample_data_row_count | 22690
| |
|
GSM238322 | GPL339 |
|
monolayer_3T3_EN_transduced, sample 2, replicate 1
|
ETV6-NTRK3 transduced NIH 3T3 cells
|
3T3 cells growing as a monolayer
|
Gene expression data from EN-transduced 3T3 cells
|
Sample_geo_accession | GSM238322
| Sample_status | Public on Dec 14 2007
| Sample_submission_date | Oct 17 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted by using Trizol following the manufacturar's instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430A chips. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip.
| Sample_platform_id | GPL339
| Sample_contact_name | Zhe,,Li
| Sample_contact_email | li@bloodgroup.tch.harvard.edu
| Sample_contact_phone | 617-919-2052
| Sample_contact_laboratory | Stuart Orkin's Lab
| Sample_contact_department | Division of Hematology/Oncology
| Sample_contact_institute | Children's Hospital Boston
| Sample_contact_address |
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238322/suppl/GSM238322.CEL.gz
| Sample_series_id | GSE9354
| Sample_data_row_count | 22690
| |
|
GSM238323 | GPL339 |
|
monolayer_3T3_EN_transduced, sample 2, replicate 2
|
ETV6-NTRK3 transduced NIH 3T3 cells
|
3T3 cells growing as a monolayer
|
Gene expression data from EN-transduced 3T3 cells
|
Sample_geo_accession | GSM238323
| Sample_status | Public on Dec 14 2007
| Sample_submission_date | Oct 17 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted by using Trizol following the manufacturar's instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430A chips. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip.
| Sample_platform_id | GPL339
| Sample_contact_name | Zhe,,Li
| Sample_contact_email | li@bloodgroup.tch.harvard.edu
| Sample_contact_phone | 617-919-2052
| Sample_contact_laboratory | Stuart Orkin's Lab
| Sample_contact_department | Division of Hematology/Oncology
| Sample_contact_institute | Children's Hospital Boston
| Sample_contact_address |
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238323/suppl/GSM238323.CEL.gz
| Sample_series_id | GSE9354
| Sample_data_row_count | 22690
| |
|
GSM238324 | GPL339 |
|
monolayer_3T3_EN-KD_transduced, replicate 1
|
NIH 3T3 cells transduced by a kinase dead (KD) version of ETV6-NTRK3
|
3T3 cells growing as a monolayer
|
Gene expression data from control 3T3 cells
|
Sample_geo_accession | GSM238324
| Sample_status | Public on Dec 14 2007
| Sample_submission_date | Oct 17 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted by using Trizol following the manufacturar's instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430A chips. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip.
| Sample_platform_id | GPL339
| Sample_contact_name | Zhe,,Li
| Sample_contact_email | li@bloodgroup.tch.harvard.edu
| Sample_contact_phone | 617-919-2052
| Sample_contact_laboratory | Stuart Orkin's Lab
| Sample_contact_department | Division of Hematology/Oncology
| Sample_contact_institute | Children's Hospital Boston
| Sample_contact_address |
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238324/suppl/GSM238324.CEL.gz
| Sample_series_id | GSE9354
| Sample_data_row_count | 22690
| |
|
GSM238325 | GPL339 |
|
monolayer_3T3_EN-KD_transduced, replicate 2
|
NIH 3T3 cells transduced by a kinase dead (KD) version of ETV6-NTRK3
|
3T3 cells growing as a monolayer
|
Gene expression data from control 3T3 cells
|
Sample_geo_accession | GSM238325
| Sample_status | Public on Dec 14 2007
| Sample_submission_date | Oct 17 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted by using Trizol following the manufacturar's instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430A chips. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip.
| Sample_platform_id | GPL339
| Sample_contact_name | Zhe,,Li
| Sample_contact_email | li@bloodgroup.tch.harvard.edu
| Sample_contact_phone | 617-919-2052
| Sample_contact_laboratory | Stuart Orkin's Lab
| Sample_contact_department | Division of Hematology/Oncology
| Sample_contact_institute | Children's Hospital Boston
| Sample_contact_address |
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238325/suppl/GSM238325.CEL.gz
| Sample_series_id | GSE9354
| Sample_data_row_count | 22690
| |
|
GSM238326 | GPL339 |
|
monolayer_3T3_Vector_transduced, replicate 1
|
empty retroviral vector transduced NIH 3T3 cells
|
3T3 cells growing as a monolayer
|
Gene expression data from control 3T3 cells
|
Sample_geo_accession | GSM238326
| Sample_status | Public on Dec 14 2007
| Sample_submission_date | Oct 17 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted by using Trizol following the manufacturar's instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430A chips. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip.
| Sample_platform_id | GPL339
| Sample_contact_name | Zhe,,Li
| Sample_contact_email | li@bloodgroup.tch.harvard.edu
| Sample_contact_phone | 617-919-2052
| Sample_contact_laboratory | Stuart Orkin's Lab
| Sample_contact_department | Division of Hematology/Oncology
| Sample_contact_institute | Children's Hospital Boston
| Sample_contact_address |
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238326/suppl/GSM238326.CEL.gz
| Sample_series_id | GSE9354
| Sample_data_row_count | 22690
| |
|
GSM238327 | GPL339 |
|
monolayer_3T3_Vector_transduced, replicate 2
|
empty retroviral vector transduced NIH 3T3 cells
|
3T3 cells growing as a monolayer
|
Gene expression data from control 3T3 cells
|
Sample_geo_accession | GSM238327
| Sample_status | Public on Dec 14 2007
| Sample_submission_date | Oct 17 2007
| Sample_last_update_date | Aug 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted by using Trizol following the manufacturar's instruction.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430A chips. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip.
| Sample_platform_id | GPL339
| Sample_contact_name | Zhe,,Li
| Sample_contact_email | li@bloodgroup.tch.harvard.edu
| Sample_contact_phone | 617-919-2052
| Sample_contact_laboratory | Stuart Orkin's Lab
| Sample_contact_department | Division of Hematology/Oncology
| Sample_contact_institute | Children's Hospital Boston
| Sample_contact_address |
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM238nnn/GSM238327/suppl/GSM238327.CEL.gz
| Sample_series_id | GSE9354
| Sample_data_row_count | 22690
| |
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