Search results for the GEO ID: GSE9521  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM241204 | GPL1261 |
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Thy1+ 2dpp gonocytes control-1
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Thy1+ 2dpp gonocytes 8hr control-1
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129/c57BL/6 male, thy1+ gonocytes isolated from 2 dpp testes and treated with vehicle (ethanol) for 8hr
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To detect changes in expression levels for gene transcripts, we used the Mouse Genome 430v2.0 GeneChip™ array (Affymetrix, Santa Clara, CA). This array contains more than 45,000 probe sets representing 39,000 transcripts and variants, and is currently the most comprehensive GeneChip array available for the mouse. Approximately 3.75 ìg of biotin-labeled, fragmented cDNA was hybridized to each array for 18 h at 45 °C. Washes and post hybridization processing were performed using the Fluidics Station 450 (Affymetrix) according to the manufacturers’ instructions, and the GeneChip™ arrays were scanned using a laser confocal slide scanner (GeneChip Scanner 3000, Affymetrix). Array quality was assessed and images were quantified using GeneChip™ Operating Software (GCOS) v1.2 (Affymetrix).
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GSM241205 | GPL1261 |
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Thy1+ 2dpp gonocytes control-2
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Thy1+ 2dpp gonocytes 8hr control-2
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129/c57BL/6 male, thy1+ gonocytes isolated from 2 dpp testes and treated with vehicle (ethanol) for 8hr
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To detect changes in expression levels for gene transcripts, we used the Mouse Genome 430v2.0 GeneChip™ array (Affymetrix, Santa Clara, CA). This array contains more than 45,000 probe sets representing 39,000 transcripts and variants, and is currently the most comprehensive GeneChip array available for the mouse. Approximately 3.75 ìg of biotin-labeled, fragmented cDNA was hybridized to each array for 18 h at 45 °C. Washes and post hybridization processing were performed using the Fluidics Station 450 (Affymetrix) according to the manufacturers’ instructions, and the GeneChip™ arrays were scanned using a laser confocal slide scanner (GeneChip Scanner 3000, Affymetrix). Array quality was assessed and images were quantified using GeneChip™ Operating Software (GCOS) v1.2 (Affymetrix).
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GSM241206 | GPL1261 |
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Thy1+ 2dpp gonocytes RA-1
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Thy1+ 2dpp gonocytes 8hr RA-1
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129/c57BL/6 male, thy1+ gonocytes isolated from 2 dpp testes and treated with RA for 8hr
|
To detect changes in expression levels for gene transcripts, we used the Mouse Genome 430v2.0 GeneChip™ array (Affymetrix, Santa Clara, CA). This array contains more than 45,000 probe sets representing 39,000 transcripts and variants, and is currently the most comprehensive GeneChip array available for the mouse. Approximately 3.75 ìg of biotin-labeled, fragmented cDNA was hybridized to each array for 18 h at 45 °C. Washes and post hybridization processing were performed using the Fluidics Station 450 (Affymetrix) according to the manufacturers’ instructions, and the GeneChip™ arrays were scanned using a laser confocal slide scanner (GeneChip Scanner 3000, Affymetrix). Array quality was assessed and images were quantified using GeneChip™ Operating Software (GCOS) v1.2 (Affymetrix).
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GSM241207 | GPL1261 |
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Thy1+ 2dpp gonocytes RA-2
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Thy1+ 2dpp gonocytes 8hr RA-2
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129/c57BL/6 male, thy1+ gonocytes isolated from 2 dpp testes and treated with RA for 8hr
|
To detect changes in expression levels for gene transcripts, we used the Mouse Genome 430v2.0 GeneChip™ array (Affymetrix, Santa Clara, CA). This array contains more than 45,000 probe sets representing 39,000 transcripts and variants, and is currently the most comprehensive GeneChip array available for the mouse. Approximately 3.75 ìg of biotin-labeled, fragmented cDNA was hybridized to each array for 18 h at 45 °C. Washes and post hybridization processing were performed using the Fluidics Station 450 (Affymetrix) according to the manufacturers’ instructions, and the GeneChip™ arrays were scanned using a laser confocal slide scanner (GeneChip Scanner 3000, Affymetrix). Array quality was assessed and images were quantified using GeneChip™ Operating Software (GCOS) v1.2 (Affymetrix).
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