Search results for the GEO ID: GSE9526  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM241222 | GPL570 |
|
cumulus cells-early cleavage-rep1a
|
cumulus cells from oocyte resulting in an early cleavage embryo (i.e. cleaved between 23-26h post-injection or 25-28h post-insemination) from patient 1
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241222
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241222/suppl/GSM241222.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241223 | GPL570 |
|
cumulus cells-early cleavage-rep1b
|
cumulus cells from oocyte resulting in an early cleavage embryo (i.e. cleaved between 23-26h post-injection or 25-28h post-insemination) from patient 1
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241223
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241223/suppl/GSM241223.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241224 | GPL570 |
|
cumulus cells-early cleavage-rep2
|
cumulus cells from oocyte resulting in an early cleavage embryo (i.e. cleaved between 23-26h post-injection or 25-28h post-insemination) from patient 2
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241224
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241224/suppl/GSM241224.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241225 | GPL570 |
|
cumulus cells-early cleavage-rep3
|
cumulus cells from oocyte resulting in an early cleavage embryo (i.e. cleaved between 23-26h post-injection or 25-28h post-insemination) from patient 3
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241225
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241225/suppl/GSM241225.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241226 | GPL570 |
|
cumulus cells-early cleavage-rep4
|
cumulus cells from oocyte resulting in an early cleavage embryo (i.e. cleaved between 23-26h post-injection or 25-28h post-insemination) from patient 4
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241226
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241226/suppl/GSM241226.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241227 | GPL570 |
|
cumulus cells-early cleavage-rep5
|
cumulus cells from oocyte resulting in an early cleavage embryo (i.e. cleaved between 23-26h post-injection or 25-28h post-insemination) from patient 5
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241227
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241227/suppl/GSM241227.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241228 | GPL570 |
|
cumulus cells-early cleavage-rep6a
|
cumulus cells from oocyte resulting in an early cleavage embryo (i.e. cleaved between 23-26h post-injection or 25-28h post-insemination) from patient 6
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241228
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241228/suppl/GSM241228.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241229 | GPL570 |
|
cumulus cells-early cleavage-rep6b
|
cumulus cells from oocyte resulting in an early cleavage embryo (i.e. cleaved between 23-26h post-injection or 25-28h post-insemination) from patient 6
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241229
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241229/suppl/GSM241229.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241230 | GPL570 |
|
cumulus cells-late cleavage-rep1a
|
cumulus cells from oocyte resulting in a late cleavage embryo from patient 1
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241230
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241230/suppl/GSM241230.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241231 | GPL570 |
|
cumulus cells-late cleavage-rep1b
|
cumulus cells from oocyte resulting in a late cleavage embryo from patient 1
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241231
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241231/suppl/GSM241231.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241232 | GPL570 |
|
cumulus cells-late cleavage-rep2
|
cumulus cells from oocyte resulting in a late cleavage embryo from patient 2
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241232
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241232/suppl/GSM241232.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241233 | GPL570 |
|
cumulus cells-late cleavage-rep3
|
cumulus cells from oocyte resulting in a late cleavage embryo from patient 3
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241233
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241233/suppl/GSM241233.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241234 | GPL570 |
|
cumulus cells-late cleavage-rep4
|
cumulus cells from oocyte resulting in a late cleavage embryo from patient 4
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241234
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241234/suppl/GSM241234.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241235 | GPL570 |
|
cumulus cells-late cleavage-rep5
|
cumulus cells from oocyte resulting in a late cleavage embryo from patient 5
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241235
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241235/suppl/GSM241235.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241236 | GPL570 |
|
cumulus cells-late cleavage-rep6a
|
cumulus cells from oocyte resulting in a late cleavage embryo from patient 6
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241236
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241236/suppl/GSM241236.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
GSM241237 | GPL570 |
|
cumulus cells-late cleavage-rep6b
|
cumulus cells from oocyte resulting in a late cleavage embryo from patient 6
|
The oocyte enclosed by these cumulus cells was fertilized normally (2PN) and developed into a 4-cell embryo with good morphology on day 2 and no multinucleated blastomeres present.
|
Comparison of cumulus cells from eight oocytes resulting in an early cleavage embryo (EC-CC; n=8) and from eight oocytes resulting in a non-EC embryo (NEC-CC; n=8).
|
| Sample_geo_accession | GSM241237
| | Sample_status | Public on Nov 10 2007
| | Sample_submission_date | Nov 06 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cumulus cells were immediately lysed in 100 ml Trizol reagent (Invitrogen, Carlsbad, USA) supplemented with 1% (v/v) 2-mercapto-ethanol (Merck, Darmstadt, Germany), snap-frozen in liquid nitrogen and stored at -80°C (cumulus cells from one oocyte per vial).
| | Sample_growth_protocol_ch1 | In consenting IVF patients, immediately following ultrasound-guided cumulus-oocyte-complex (COC) retrieval, a proportion of the cumulus cells surrounding a single oocyte were removed using a sharp needle.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturers’ instructions with some adaptations for the small quantity of RNA. RNA was precipitated with isopropyl alcohol for 2h and the RNA pellet was washed three times with 75% ethanol. To be able to track the small RNA pellet, 5 mg glycogen (Ambion, Woodward, USA) was added to the sample before RNA precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fifty ng total RNA was amplified using the two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, USA) in combination with the MEGAscript T7 in vitro transcription system (Ambion) and biotin labeled with GeneChip IVT labeling kit (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G.
| | Sample_data_processing | Affymetrix GCOS, TGT value: 500, Norm Factor: 1
| | Sample_platform_id | GPL570
| | Sample_contact_name | Aafke,,van Montfoort
| | Sample_contact_email | avmn@sgyn.azm.nl
| | Sample_contact_laboratory | IVF laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Academic hospital Maastricht
| | Sample_contact_address | P.O.Box 5800
| | Sample_contact_city | maastricht
| | Sample_contact_zip/postal_code | 6202 AZ
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM241nnn/GSM241237/suppl/GSM241237.CEL.gz
| | Sample_series_id | GSE9526
| | Sample_data_row_count | 54675
| | |
|
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