Search results for the GEO ID: GSE9593  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM242185 | GPL570 |
|
BM-MSC_donor1(#285)-P2-44yrs
|
bone marrow mesenchymal stem cells of donor1 - passage 2
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 2
44 year old donor
|
Donor1(#285)-P2-44yrs
|
| Sample_geo_accession | GSM242185
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 12 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 2
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., BLOOD, 2001).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., EXP HEMATOL 2005;Wagner et al., EXP HEMATOL 2006). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242185/suppl/GSM242185.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242185/suppl/GSM242185.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_series_id | GSE12274
| | Sample_data_row_count | 54675
| | |
|
GSM242650 | GPL570 |
|
BM-MSC_donor1-P3
|
bone marrow mesenchymal stem cells of donor1 - passage 3
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 3
|
Donor1-P3
|
| Sample_geo_accession | GSM242650
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 3
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242650/suppl/GSM242650.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242650/suppl/GSM242650.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242651 | GPL570 |
|
BM-MSC_donor1-P5
|
bone marrow mesenchymal stem cells of donor1 - passage 5
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 5
|
Donor1-P5
|
| Sample_geo_accession | GSM242651
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 5
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242651/suppl/GSM242651.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242651/suppl/GSM242651.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242652 | GPL570 |
|
BM-MSC_donor1-P6
|
bone marrow mesenchymal stem cells of donor1 - passage 6
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 6
|
Donor1-P6
|
| Sample_geo_accession | GSM242652
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 6
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242652/suppl/GSM242652.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242652/suppl/GSM242652.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242653 | GPL570 |
|
BM-MSC_donor1-P7
|
bone marrow mesenchymal stem cells of donor1 - passage 7
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 7
|
Donor1-P7
|
| Sample_geo_accession | GSM242653
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 7
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242653/suppl/GSM242653.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242653/suppl/GSM242653.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242666 | GPL570 |
|
BM-MSC_donor1-P8
|
bone marrow mesenchymal stem cells of donor1 - passage 8
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 8
|
Donor1-P8
|
| Sample_geo_accession | GSM242666
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 8
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242666/suppl/GSM242666.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242666/suppl/GSM242666.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242667 | GPL570 |
|
BM-MSC_donor1-P10
|
bone marrow mesenchymal stem cells of donor1 - passage 10
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 10
|
Donor1-P10
|
| Sample_geo_accession | GSM242667
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 10
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242667/suppl/GSM242667.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242667/suppl/GSM242667.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242668 | GPL570 |
|
BM-MSC_donor1-P11
|
bone marrow mesenchymal stem cells of donor1 - passage 11
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 11
|
Donor1-P11
|
| Sample_geo_accession | GSM242668
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 11
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242668/suppl/GSM242668.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242668/suppl/GSM242668.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242669 | GPL570 |
|
BM-MSC_donor2(#287)-P2-55yrs
|
bone marrow mesenchymal stem cells of donor2 - passage 2
|
mesenchymal stem cells from human bone marrow (donor 2)
(alternatively named mesenchymal stromal cells)
Passage 2
55 year old donor
|
Donor2-P2-55yrs
|
| Sample_geo_accession | GSM242669
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 2
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242669/suppl/GSM242669.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242669/suppl/GSM242669.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_series_id | GSE12274
| | Sample_data_row_count | 54675
| | |
|
GSM242672 | GPL570 |
|
BM-MSC_donor2-P7
|
bone marrow mesenchymal stem cells of donor2 - passage 7
|
mesenchymal stem cells from human bone marrow (donor 2)
(alternatively named mesenchymal stromal cells)
Passage 7 (senescent passage)
|
Donor2-P7
|
| Sample_geo_accession | GSM242672
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 7
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242672/suppl/GSM242672.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242672/suppl/GSM242672.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242673 | GPL570 |
|
BM-MSC_donor3(#288)-P2-24yrs
|
bone marrow mesenchymal stem cells of donor3 - passage 2
|
mesenchymal stem cells from human bone marrow (donor 3)
(alternatively named mesenchymal stromal cells)
Passage 2
24 year old donor
|
Donor3-P2-24yrs
|
| Sample_geo_accession | GSM242673
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 2
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242673/suppl/GSM242673.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242673/suppl/GSM242673.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_series_id | GSE12274
| | Sample_data_row_count | 54675
| | |
|
GSM242674 | GPL570 |
|
BM-MSC_donor3-P8
|
bone marrow mesenchymal stem cells of donor3 - passage 8
|
mesenchymal stem cells from human bone marrow (donor 3)
(alternatively named mesenchymal stromal cells)
Passage 8 (senescent passage)
|
Donor3-P8
|
| Sample_geo_accession | GSM242674
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 8
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology.
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242674/suppl/GSM242674.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242674/suppl/GSM242674.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
| | |
|
GSM242675 | GPL570 |
|
BM-MSC_donor1-P4
|
bone marrow mesenchymal stem cells of donor1 - passage 4
|
mesenchymal stem cells from human bone marrow
(alternatively named mesenchymal stromal cells)
Passage 4
|
Donor1-P4
|
| Sample_geo_accession | GSM242675
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Nov 13 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | University of Heidelberg
| | Sample_treatment_protocol_ch1 | Cells were harvested by trypsination after passage 4
| | Sample_growth_protocol_ch1 | Human bone marrow (BM) samples were taken after informed consent using guidelines approved by the Ethic Committee on the Use of Human Subjects at the University of Heidelberg. Mesenchymal stem cells (MSC) were isolated in the culture medium described by M. Reyes and colleagues (Reyes et al., 2001, Blood, 98, 2615-2625).
| | Sample_growth_protocol_ch1 | The medium consists of 58% Dulbecco´s Modified Eagles Medium-Low Glucose (DMEM-LG, Cambrex, Apen, Germany) and 40% MCDB201 (Sigma, Deisenhofen, Germany), 2% FCS (HyClone, Bonn, Germany), supplemented with 2 mM L-Glutamine, 100 U/ml Pen/Strep (Cambrex), 1% insulin transferrin selenium, 1% linoleic acid bovine serum albumin, 10 nM dexamethasone, 0.1 mM L-ascorbic-acid-2-phosphate (all from Sigma), PDGF-bb and EGF (10ng/ml each, R&D Systems, Wiesbaden, Germany). Tissue culture flasks were coated with 10 ng/ml fibronectin (Sigma) before use (Wagner et al., 2005, Exp Hematol, 33, 1402-1416; Wagner et al., Exp Hematol, 34, 536-548). MSC were cultured at 37°C in a humidified atmosphere containing 5% carbon dioxide with medium changes twice a week. After 7-10 days initial colonies were replated in a new culture flask (passage 1, P1). Upon subconfluent growth (70%) cells were always replated at a density of 10(4) cells/cm(2).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen, Paisley, Scotland) according to the manufacturer´s instructions. RNA quality was controlled using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany) and quantified with a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Two µg total RNA was amplified with GeneChip one-cycle target labeling kit (Affymetrix, High Wycombe, United Kingdom) according to the manufacturers instructions. Quality of amplified RNA was controlled by LabChip technology.
| | Sample_hyb_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were hybridized with 15 µg amplified RNA and washed with a fluidics station 450 (Affymetrix).
| | Sample_scan_protocol | GeneChip Human Genome U133_Plus_2.0 (Affymetrix) were scanned with GeneChip scanner 3000 (Affymetrix)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wolfgang,,Wagner
| | Sample_contact_email | wwagner@ukaachen.de
| | Sample_contact_phone | +49 241 8088611
| | Sample_contact_laboratory | Stem Cell Biology and Cellular Engineering
| | Sample_contact_department | Helmholtz Institute for Biomedical Engineering
| | Sample_contact_institute | RWTH Aachen University
| | Sample_contact_address | Pauwelsstrasse 20
| | Sample_contact_city | Aachen
| | Sample_contact_zip/postal_code | 52074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242675/suppl/GSM242675.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242675/suppl/GSM242675.CHP.gz
| | Sample_series_id | GSE9593
| | Sample_data_row_count | 54675
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