Search results for the GEO ID: GSE9601  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM242894 | GPL8300 |
|
Monocyte_HCMV-1
|
HCMV-infected monocytes at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte, biological rep1
|
| Sample_geo_accession | GSM242894
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242894/suppl/GSM242894.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242894/suppl/GSM242894.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_series_id | GSE11408
| | Sample_data_row_count | 12625
| | |
|
GSM242895 | GPL8300 |
|
Monocyte_HCMV-2
|
HCMV-infected monocytes at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte, biological rep2
|
| Sample_geo_accession | GSM242895
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242895/suppl/GSM242895.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242895/suppl/GSM242895.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_series_id | GSE11408
| | Sample_data_row_count | 12625
| | |
|
GSM242896 | GPL8300 |
|
Monocyte_HCMV-3
|
HCMV-infected monocytes at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte, biological rep3
|
| Sample_geo_accession | GSM242896
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242896/suppl/GSM242896.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242896/suppl/GSM242896.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_series_id | GSE11408
| | Sample_data_row_count | 12625
| | |
|
GSM242897 | GPL8300 |
|
Monocyte_HCMV-4
|
HCMV-infected monocytes at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte, biological rep4
|
| Sample_geo_accession | GSM242897
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242897/suppl/GSM242897.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242897/suppl/GSM242897.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_series_id | GSE11408
| | Sample_data_row_count | 12625
| | |
|
GSM242898 | GPL8300 |
|
Monocyte_HCMV-5
|
HCMV-infected monocytes at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte, biological rep5
|
| Sample_geo_accession | GSM242898
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242898/suppl/GSM242898.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242898/suppl/GSM242898.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_series_id | GSE11408
| | Sample_data_row_count | 12625
| | |
|
GSM242899 | GPL8300 |
|
Monocyte_HCMV-6
|
HCMV-infected monocytes at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte, biological rep6
|
| Sample_geo_accession | GSM242899
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242899/suppl/GSM242899.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242899/suppl/GSM242899.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_series_id | GSE11408
| | Sample_data_row_count | 12625
| | |
|
GSM242900 | GPL8300 |
|
Monocyte_HCMV_B11-1
|
HCMV-infected monocyte pretreated with Bay11 at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte pretreated with Bay11, biological rep1
|
| Sample_geo_accession | GSM242900
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242900/suppl/GSM242900.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242900/suppl/GSM242900.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_data_row_count | 12625
| | |
|
GSM242901 | GPL8300 |
|
Monocyte_HCMV_B11-2
|
HCMV-infected monocyte pretreated with Bay11 at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte pretreated with Bay11, biological rep2
|
| Sample_geo_accession | GSM242901
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242901/suppl/GSM242901.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242901/suppl/GSM242901.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_data_row_count | 12625
| | |
|
GSM242902 | GPL8300 |
|
Monocyte_HCMV_B11-3
|
HCMV-infected monocyte pretreated with Bay11 at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte pretreated with Bay11, biological rep3
|
| Sample_geo_accession | GSM242902
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242902/suppl/GSM242902.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242902/suppl/GSM242902.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_data_row_count | 12625
| | |
|
GSM242903 | GPL8300 |
|
Monocyte_HCMV_LY-1
|
HCMV-infected monocyte pretreated with LY at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte pretreated with LY, biological rep1
|
| Sample_geo_accession | GSM242903
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242903/suppl/GSM242903.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242903/suppl/GSM242903.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_data_row_count | 12625
| | |
|
GSM242904 | GPL8300 |
|
Monocyte_HCMV_LY-2
|
HCMV-infected monocyte pretreated with LY at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte pretreated with LY, biological rep2
|
| Sample_geo_accession | GSM242904
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242904/suppl/GSM242904.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242904/suppl/GSM242904.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_data_row_count | 12625
| | |
|
GSM242905 | GPL8300 |
|
Monocyte_HCMV_LY-3
|
HCMV-infected monocyte pretreated with LY at 4 hours post infection
|
Tissue: Peripheral blood
Virus Strain: Towne/E
|
HCMV-infected monocyte pretreated with LY, biological rep3
|
| Sample_geo_accession | GSM242905
| | Sample_status | Public on Nov 15 2007
| | Sample_submission_date | Nov 14 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
| | Sample_growth_protocol_ch1 | Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Andrew,D.,Yurochko
| | Sample_contact_email | ayuroc@lsuhsc.edu
| | Sample_contact_phone | 318-675-8332
| | Sample_contact_fax | 318-675-5764
| | Sample_contact_department | Microbiology & Immunology
| | Sample_contact_institute | LSUHSC-S
| | Sample_contact_address | 1501 Kings Highway
| | Sample_contact_city | Shreveport
| | Sample_contact_state | LA
| | Sample_contact_zip/postal_code | 71130-3932
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242905/suppl/GSM242905.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM242nnn/GSM242905/suppl/GSM242905.CHP.gz
| | Sample_series_id | GSE9601
| | Sample_data_row_count | 12625
| | |
|
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