Result

Search results for the GEO ID: GSE9659

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GSM ID

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Title

Source name

Description

Characteristics

GSM244087

GPL85

RL120502-control

brain; control

CNS mixed glial cultures from 2-3 day old Sprague-Dawley rats

Maintained in defined medium with 2% fetal bovine serum

Sample_geo_accession	GSM244087
Sample_status	Public on Nov 07 2008
Sample_submission_date	Nov 21 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were maintained in defined medium with 2% fetal bovine serum for 6-8 days, then treated with cytokine mixtures representative of Th1, Th2 or monocyte/macrophage cytokines for 6 hours (Lisak, et al., Mult. Scler. 12:149-168, 2006)
Sample_growth_protocol_ch1	Mixed CNS glial cultures were prepared from neonatal rat brain using a modification of the shakeoff technique (McCarthy and deVellis, J. Cell. Biol. 85:890-902, 1980; Dyer and Benjamins, J. Neurosci. 8:4307-4318, 1988)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Rat RG-U34A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Agilent GeneArray Scanner. Normalization Factor- 1.0, TGT=500 (all probe sets)
Sample_data_processing	All cel files were read and normalized by dChip v1.2, normalized data were analysed by GeneSpring software
Sample_platform_id	GPL85
Sample_contact_name	wenbo,,xu
Sample_contact_institute	wayne state university
Sample_contact_address	3127 Scott Hall ,540 East Canfield
Sample_contact_city	Detroit
Sample_contact_state	MI
Sample_contact_zip/postal_code	48201
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM244nnn/GSM244087/suppl/GSM244087.CEL.gz
Sample_series_id	GSE9659
Sample_data_row_count	8799

GSM244088

GPL85

RL120502-mono macro

brain; treated with cytokines typical of monocyte/macrophages

CNS mixed glial cultures from 2-3 day old Sprague-Dawley rats

Treated with cytokines typical of monocyte/macrophages for 6 hours; recombinant rat IL1a, IL1b, IL-12p40, and TNF-a, all at 10 ng/ml in defined medium with 2% fetal bovine serum.

Sample_geo_accession	GSM244088
Sample_status	Public on Nov 07 2008
Sample_submission_date	Nov 21 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were maintained in defined medium with 2% fetal bovine serum for 6-8 days, then treated with cytokine mixtures representative of Th1, Th2 or monocyte/macrophage cytokines for 6 hours (Lisak, et al., Mult. Scler. 12:149-168, 2006)
Sample_growth_protocol_ch1	Mixed CNS glial cultures were prepared from neonatal rat brain using a modification of the shakeoff technique (McCarthy and deVellis, J. Cell. Biol. 85:890-902, 1980; Dyer and Benjamins, J. Neurosci. 8:4307-4318, 1988)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Rat RG-U34A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Agilent GeneArray Scanner. Normalization Factor- 1.0, TGT=500 (all probe sets)
Sample_data_processing	All cel files were read and normalized by dChip v1.2, normalized data were analysed by GeneSpring software
Sample_platform_id	GPL85
Sample_contact_name	wenbo,,xu
Sample_contact_institute	wayne state university
Sample_contact_address	3127 Scott Hall ,540 East Canfield
Sample_contact_city	Detroit
Sample_contact_state	MI
Sample_contact_zip/postal_code	48201
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM244nnn/GSM244088/suppl/GSM244088.CEL.gz
Sample_series_id	GSE9659
Sample_data_row_count	8799

GSM244089

GPL85

RL120502-Th1

brain; treated with cytokines typical of Th1 cells

CNS mixed glial cultures from 2-3 day old Sprague-Dawley rats

Treated with cytokines typical of Th1 cells for 6 hours; recombinant rat IL-2, interferon-g, TNF-a, and g-CSF, all at 10 ng/ml in defined medium with 2% fetal bovine serum.

Sample_geo_accession	GSM244089
Sample_status	Public on Nov 07 2008
Sample_submission_date	Nov 21 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were maintained in defined medium with 2% fetal bovine serum for 6-8 days, then treated with cytokine mixtures representative of Th1, Th2 or monocyte/macrophage cytokines for 6 hours (Lisak, et al., Mult. Scler. 12:149-168, 2006)
Sample_growth_protocol_ch1	Mixed CNS glial cultures were prepared from neonatal rat brain using a modification of the shakeoff technique (McCarthy and deVellis, J. Cell. Biol. 85:890-902, 1980; Dyer and Benjamins, J. Neurosci. 8:4307-4318, 1988)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Rat RG-U34A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Agilent GeneArray Scanner. Normalization Factor- 1.0, TGT=500 (all probe sets)
Sample_data_processing	All cel files were read and normalized by dChip v1.2, normalized data were analysed by GeneSpring software
Sample_platform_id	GPL85
Sample_contact_name	wenbo,,xu
Sample_contact_institute	wayne state university
Sample_contact_address	3127 Scott Hall ,540 East Canfield
Sample_contact_city	Detroit
Sample_contact_state	MI
Sample_contact_zip/postal_code	48201
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM244nnn/GSM244089/suppl/GSM244089.CEL.gz
Sample_series_id	GSE9659
Sample_data_row_count	8799

GSM244090

GPL85

RL120502-Th2

brain; treated with cytokines typical of Th2 cells

CNS mixed glial cultures from 2-3 day old Sprague-Dawley rats

Treated with cytokines typical of Th2 cells for 6 hours; recombinant rat IL-5, IL-10, G-CSF and porcine TGF-b1, all at 10 ng/ml in defined medium with 2% fetal bovine serum.

Sample_geo_accession	GSM244090
Sample_status	Public on Nov 07 2008
Sample_submission_date	Nov 21 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were maintained in defined medium with 2% fetal bovine serum for 6-8 days, then treated with cytokine mixtures representative of Th1, Th2 or monocyte/macrophage cytokines for 6 hours (Lisak, et al., Mult. Scler. 12:149-168, 2006)
Sample_growth_protocol_ch1	Mixed CNS glial cultures were prepared from neonatal rat brain using a modification of the shakeoff technique (McCarthy and deVellis, J. Cell. Biol. 85:890-902, 1980; Dyer and Benjamins, J. Neurosci. 8:4307-4318, 1988)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Rat RG-U34A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Agilent GeneArray Scanner. Normalization Factor- 1.0, TGT=500 (all probe sets)
Sample_data_processing	All cel files were read and normalized by dChip v1.2, normalized data were analysed by GeneSpring software
Sample_platform_id	GPL85
Sample_contact_name	wenbo,,xu
Sample_contact_institute	wayne state university
Sample_contact_address	3127 Scott Hall ,540 East Canfield
Sample_contact_city	Detroit
Sample_contact_state	MI
Sample_contact_zip/postal_code	48201
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM244nnn/GSM244090/suppl/GSM244090.CEL.gz
Sample_series_id	GSE9659
Sample_data_row_count	8799

GSM244091

GPL85

BB082802-control

brain; control

CNS mixed glial cultures from 2-3 day old Sprague-Dawley rats

Maintained in defined medium with 2% fetal bovine serum

Sample_geo_accession	GSM244091
Sample_status	Public on Nov 07 2008
Sample_submission_date	Nov 21 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were maintained in defined medium with 2% fetal bovine serum for 6-8 days, then treated with cytokine mixtures representative of Th1, Th2 or monocyte/macrophage cytokines for 6 hours (Lisak, et al., Mult. Scler. 12:149-168, 2006)
Sample_growth_protocol_ch1	Mixed CNS glial cultures were prepared from neonatal rat brain using a modification of the shakeoff technique (McCarthy and deVellis, J. Cell. Biol. 85:890-902, 1980; Dyer and Benjamins, J. Neurosci. 8:4307-4318, 1988)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Rat RG-U34A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Agilent GeneArray Scanner. Normalization Factor- 1.0, TGT=500 (all probe sets)
Sample_data_processing	All cel files were read and normalized by dChip v1.2, normalized data were analysed by GeneSpring software
Sample_platform_id	GPL85
Sample_contact_name	wenbo,,xu
Sample_contact_institute	wayne state university
Sample_contact_address	3127 Scott Hall ,540 East Canfield
Sample_contact_city	Detroit
Sample_contact_state	MI
Sample_contact_zip/postal_code	48201
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM244nnn/GSM244091/suppl/GSM244091.CEL.gz
Sample_series_id	GSE9659
Sample_data_row_count	8799

GSM244092

GPL85

BB082802-mono macro

brain; treated with cytokines typical of monocyte/macrophages

CNS mixed glial cultures from 2-3 day old Sprague-Dawley rats

Treated with cytokines typical of monocyte/macrophages for 6 hours; recombinant rat IL1a, IL1b, IL-12p40, and TNF-a, all at 10 ng/ml in defined medium with 2% fetal bovine serum.

Sample_geo_accession	GSM244092
Sample_status	Public on Nov 07 2008
Sample_submission_date	Nov 21 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were maintained in defined medium with 2% fetal bovine serum for 6-8 days, then treated with cytokine mixtures representative of Th1, Th2 or monocyte/macrophage cytokines for 6 hours (Lisak, et al., Mult. Scler. 12:149-168, 2006)
Sample_growth_protocol_ch1	Mixed CNS glial cultures were prepared from neonatal rat brain using a modification of the shakeoff technique (McCarthy and deVellis, J. Cell. Biol. 85:890-902, 1980; Dyer and Benjamins, J. Neurosci. 8:4307-4318, 1988)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Rat RG-U34A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Agilent GeneArray Scanner. Normalization Factor- 1.0, TGT=500 (all probe sets)
Sample_data_processing	All cel files were read and normalized by dChip v1.2, normalized data were analysed by GeneSpring software
Sample_platform_id	GPL85
Sample_contact_name	wenbo,,xu
Sample_contact_institute	wayne state university
Sample_contact_address	3127 Scott Hall ,540 East Canfield
Sample_contact_city	Detroit
Sample_contact_state	MI
Sample_contact_zip/postal_code	48201
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM244nnn/GSM244092/suppl/GSM244092.CEL.gz
Sample_series_id	GSE9659
Sample_data_row_count	8799

GSM244093

GPL85

BB082802-Th1

brain; treated with cytokines typical of Th1 cells

CNS mixed glial cultures from 2-3 day old Sprague-Dawley rats

Treated with cytokines typical of Th1 cells for 6 hours; recombinant rat IL-2, interferon-g, TNF-a, and g-CSF, all at 10 ng/ml in defined medium with 2% fetal bovine serum.

Sample_geo_accession	GSM244093
Sample_status	Public on Nov 07 2008
Sample_submission_date	Nov 21 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were maintained in defined medium with 2% fetal bovine serum for 6-8 days, then treated with cytokine mixtures representative of Th1, Th2 or monocyte/macrophage cytokines for 6 hours (Lisak, et al., Mult. Scler. 12:149-168, 2006)
Sample_growth_protocol_ch1	Mixed CNS glial cultures were prepared from neonatal rat brain using a modification of the shakeoff technique (McCarthy and deVellis, J. Cell. Biol. 85:890-902, 1980; Dyer and Benjamins, J. Neurosci. 8:4307-4318, 1988)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Rat RG-U34A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Agilent GeneArray Scanner. Normalization Factor- 1.0, TGT=500 (all probe sets)
Sample_data_processing	All cel files were read and normalized by dChip v1.2, normalized data were analysed by GeneSpring software
Sample_platform_id	GPL85
Sample_contact_name	wenbo,,xu
Sample_contact_institute	wayne state university
Sample_contact_address	3127 Scott Hall ,540 East Canfield
Sample_contact_city	Detroit
Sample_contact_state	MI
Sample_contact_zip/postal_code	48201
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM244nnn/GSM244093/suppl/GSM244093.CEL.gz
Sample_series_id	GSE9659
Sample_data_row_count	8799

GSM244094

GPL85

BB082802-Th2

brain; treated with cytokines typical of Th2 cells

CNS mixed glial cultures from 2-3 day old Sprague-Dawley rats

Treated with cytokines typical of Th2 cells for 6 hours; recombinant rat IL-5, IL-10, G-CSF and porcine TGF-b1, all at 10 ng/ml in defined medium with 2% fetal bovine serum.

Sample_geo_accession	GSM244094
Sample_status	Public on Nov 07 2008
Sample_submission_date	Nov 21 2007
Sample_last_update_date	Aug 14 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were maintained in defined medium with 2% fetal bovine serum for 6-8 days, then treated with cytokine mixtures representative of Th1, Th2 or monocyte/macrophage cytokines for 6 hours (Lisak, et al., Mult. Scler. 12:149-168, 2006)
Sample_growth_protocol_ch1	Mixed CNS glial cultures were prepared from neonatal rat brain using a modification of the shakeoff technique (McCarthy and deVellis, J. Cell. Biol. 85:890-902, 1980; Dyer and Benjamins, J. Neurosci. 8:4307-4318, 1988)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Rat RG-U34A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Agilent GeneArray Scanner. Normalization Factor- 1.0, TGT=500 (all probe sets)
Sample_data_processing	All cel files were read and normalized by dChip v1.2, normalized data were analysed by GeneSpring software
Sample_platform_id	GPL85
Sample_contact_name	wenbo,,xu
Sample_contact_institute	wayne state university
Sample_contact_address	3127 Scott Hall ,540 East Canfield
Sample_contact_city	Detroit
Sample_contact_state	MI
Sample_contact_zip/postal_code	48201
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM244nnn/GSM244094/suppl/GSM244094.CEL.gz
Sample_series_id	GSE9659
Sample_data_row_count	8799

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