Search results for the GEO ID: GSE9727  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM245893 | GPL339 |
|
D- S49_untreated_time0, biological rep1
|
S49 D- cells untreated
|
Balb/C, Lymphoma, tumor
|
Untreated (time 0) D- S49 cells, biological rep1
|
| Sample_geo_accession | GSM245893
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245893/suppl/GSM245893.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245894 | GPL339 |
|
D- S49_untreated_time0, biological rep2
|
S49 D- cells untreated
|
Balb/C, Lymphoma, tumor
|
Untreated (time 0) D- S49 cells, biological rep2
|
| Sample_geo_accession | GSM245894
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245894/suppl/GSM245894.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245895 | GPL339 |
|
D- S49_untreated_time0, biological rep3
|
S49 D- cells untreated
|
Balb/C, Lymphoma, tumor
|
Untreated (time 0) D- S49 cells, biological rep3
|
| Sample_geo_accession | GSM245895
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245895/suppl/GSM245895.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245896 | GPL339 |
|
D- S49_untreated_time0, biological rep4
|
S49 D- cells untreated
|
Balb/C, Lymphoma, tumor
|
Untreated (time 0) D- S49 cells, biological rep4
|
| Sample_geo_accession | GSM245896
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245896/suppl/GSM245896.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245897 | GPL339 |
|
D- S49_treated_2h, biological rep1
|
S49 D- cells 2h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
2h 8-CPT-cAMP treated D- S49 cells, biological rep1
|
| Sample_geo_accession | GSM245897
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245897/suppl/GSM245897.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245898 | GPL339 |
|
D- S49_treated_2h, biological rep2
|
S49 D- cells 2h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
2h 8-CPT-cAMP treated D- S49 cells, biological rep2
|
| Sample_geo_accession | GSM245898
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245898/suppl/GSM245898.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245899 | GPL339 |
|
D- S49_treated_2h, biological rep3
|
S49 D- cells 2h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
2h 8-CPT-cAMP treated D- S49 cells, biological rep3
|
| Sample_geo_accession | GSM245899
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245899/suppl/GSM245899.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245900 | GPL339 |
|
D- S49_treated_6h, biological rep1
|
S49 D- cells 6h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
6h 8-CPT-cAMP treated D- S49 cells, biological rep1
|
| Sample_geo_accession | GSM245900
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245900/suppl/GSM245900.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245901 | GPL339 |
|
D- S49_treated_6h, biological rep2
|
S49 D- cells 6h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
6h 8-CPT-cAMP treated D- S49 cells, biological rep2
|
| Sample_geo_accession | GSM245901
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245901/suppl/GSM245901.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245902 | GPL339 |
|
D- S49_treated_6h, biological rep3
|
S49 D- cells 6h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
6h 8-CPT-cAMP treated D- S49 cells, biological rep3
|
| Sample_geo_accession | GSM245902
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245902/suppl/GSM245902.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245903 | GPL339 |
|
D- S49_treated_24h, biological rep1
|
S49 D- cells 24h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
24h 8-CPT-cAMP treated D- S49 cells, biological rep1
|
| Sample_geo_accession | GSM245903
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245903/suppl/GSM245903.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245904 | GPL339 |
|
D- S49_treated_24h, biological rep2
|
S49 D- cells 24h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
24h 8-CPT-cAMP treated D- S49 cells, biological rep2
|
| Sample_geo_accession | GSM245904
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245904/suppl/GSM245904.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM245905 | GPL339 |
|
D- S49_treated_24h, biological rep3
|
S49 D- cells 24h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
24h 8-CPT-cAMP treated D- S49 cells, biological rep3
|
| Sample_geo_accession | GSM245905
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Nov 29 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM245nnn/GSM245905/suppl/GSM245905.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248218 | GPL339 |
|
WT S49_untreated_time0, biological rep1
|
S49 WT cells untreated
|
Balb/C, Lymphoma, tumor
|
Untreated (time 0) WT S49 cells, biological rep1
|
| Sample_geo_accession | GSM248218
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248218/suppl/GSM248218.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248219 | GPL339 |
|
WT S49_untreated_time0, biological rep2
|
S49 WT cells untreated
|
Balb/C, Lymphoma, tumor
|
Untreated (time 0) WT S49 cells, biological rep2
|
| Sample_geo_accession | GSM248219
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248219/suppl/GSM248219.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248220 | GPL339 |
|
WT S49_untreated_time0, biological rep3
|
S49 WT cells untreated
|
Balb/C, Lymphoma, tumor
|
Untreated (time 0) WT S49 cells, biological rep3
|
| Sample_geo_accession | GSM248220
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248220/suppl/GSM248220.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248221 | GPL339 |
|
WT S49_untreated_time0, biological rep4
|
S49 WT cells untreated
|
Balb/C, Lymphoma, tumor
|
Untreated (time 0) WT S49 cells, biological rep4
|
| Sample_geo_accession | GSM248221
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248221/suppl/GSM248221.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248222 | GPL339 |
|
WT S49_untreated_2h, biological rep1
|
S49 WT cells 2h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
2h 8-CPT-cAMP treated WT S49 cells, biological rep1
|
| Sample_geo_accession | GSM248222
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248222/suppl/GSM248222.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248223 | GPL339 |
|
WT S49_untreated_2h, biological rep2
|
S49 WT cells 2h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
2h 8-CPT-cAMP treated WT S49 cells, biological rep2
|
| Sample_geo_accession | GSM248223
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248223/suppl/GSM248223.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248224 | GPL339 |
|
WT S49_untreated_2h, biological rep3
|
S49 WT cells 2h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
2h 8-CPT-cAMP treated WT S49 cells, biological rep3
|
| Sample_geo_accession | GSM248224
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248224/suppl/GSM248224.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248225 | GPL339 |
|
WT S49_untreated_6h, biological rep1
|
S49 WT cells 6h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
6h 8-CPT-cAMP treated WT S49 cells, biological rep1
|
| Sample_geo_accession | GSM248225
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248225/suppl/GSM248225.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248226 | GPL339 |
|
WT S49_untreated_6h, biological rep2
|
S49 WT cells 6h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
6h 8-CPT-cAMP treated WT S49 cells, biological rep2
|
| Sample_geo_accession | GSM248226
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248226/suppl/GSM248226.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248227 | GPL339 |
|
WT S49_untreated_6h, biological rep3
|
S49 WT cells 6h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
6h 8-CPT-cAMP treated WT S49 cells, biological rep3
|
| Sample_geo_accession | GSM248227
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248227/suppl/GSM248227.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248228 | GPL339 |
|
WT S49_untreated_24h, biological rep1
|
S49 WT cells 24h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
24h 8-CPT-cAMP treated WT S49 cells, biological rep1
|
| Sample_geo_accession | GSM248228
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248228/suppl/GSM248228.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248229 | GPL339 |
|
WT S49_untreated_24h, biological rep2
|
S49 WT cells 24h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
24h 8-CPT-cAMP treated WT S49 cells, biological rep2
|
| Sample_geo_accession | GSM248229
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248229/suppl/GSM248229.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
GSM248230 | GPL339 |
|
WT S49_untreated_24h, biological rep3
|
S49 WT cells 24h treatment with 8-CPT-cAMP
|
Balb/C, Lymphoma, tumor
|
24h 8-CPT-cAMP treated WT S49 cells, biological rep3
|
| Sample_geo_accession | GSM248230
| | Sample_status | Public on Dec 12 2007
| | Sample_submission_date | Dec 11 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | cells were treated with 100 mM 8-CPT-cAMP for the indicated times
| | Sample_growth_protocol_ch1 | D- S49 cells were grown in suspension cultures with Dulbecco's modified Eagles' medium supplemented with 10% heat-inactivated horse serum and 10 mM HEPES in a humidified atmosphere containing 10% CO2 at 37ºC. Cultures were initiated at a density of 2.5-5 x 10E5 cells/ml and carried out for the indicated times.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated from cells by using RNeasy mini-columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cellular RNA was reverse transcribed with superscript II reverse transcriptase and an oligo-dT primer containing a T7 RNA polymerase promoter. Single stranded cDNA was converted into double-stranded cDNA (ds-cDNA) and then purified (MessageAMP aRNA Kit, Ambion, Austin, TX). Biotinylated cRNA was generated from ds-cDNA by in vitro transcription (IVT) (MessageAMP aRNA Kit, Ambion). After a further round of purification, IVT reactions yielded 30–70 µg of biotinylated cRNA, which was fragmented to ~100–base pair fragments before hybridization.
| | Sample_hyb_protocol | Each Affymetrix 430 A chip was hybridized to 10 µg of fragmented cRNA.
| | Sample_scan_protocol | Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix)
| | Sample_data_processing | Cel files were converted to signal values by gcRMA
| | Sample_platform_id | GPL339
| | Sample_contact_name | Alexander,C,Zambon
| | Sample_contact_laboratory | Paul Insel Lab
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Clalifornia, San Diego
| | Sample_contact_address | 9500 Gilman Dr.
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92093-0636
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM248nnn/GSM248230/suppl/GSM248230.CEL.gz
| | Sample_series_id | GSE9727
| | Sample_data_row_count | 22690
| | |
|
| |
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