Search results for the GEO ID: GSE9758  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM246279 | GPL570 |
|
ERa3411 rep1
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep1
|
| Sample_geo_accession | GSM246279
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246279/suppl/GSM246279.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246280 | GPL570 |
|
ERa3411 rep2
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep2
|
| Sample_geo_accession | GSM246280
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246280/suppl/GSM246280.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246281 | GPL570 |
|
ERa3411 rep3
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep3
|
| Sample_geo_accession | GSM246281
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246281/suppl/GSM246281.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246282 | GPL570 |
|
ERa3411 rep4
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep4
|
| Sample_geo_accession | GSM246282
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246282/suppl/GSM246282.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246283 | GPL570 |
|
ERa3411 rep5
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep5
|
| Sample_geo_accession | GSM246283
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246283/suppl/GSM246283.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246284 | GPL570 |
|
ERa3411 rep6
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep6
|
| Sample_geo_accession | GSM246284
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246284/suppl/GSM246284.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246285 | GPL570 |
|
CMV rep1
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep1
|
| Sample_geo_accession | GSM246285
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246285/suppl/GSM246285.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246286 | GPL570 |
|
CMV rep2
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep2
|
| Sample_geo_accession | GSM246286
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246286/suppl/GSM246286.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246287 | GPL570 |
|
CMV rep3
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep3
|
| Sample_geo_accession | GSM246287
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246287/suppl/GSM246287.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246288 | GPL570 |
|
CMV rep4
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep4
|
| Sample_geo_accession | GSM246288
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246288/suppl/GSM246288.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246289 | GPL570 |
|
CMV rep5
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep5
|
| Sample_geo_accession | GSM246289
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246289/suppl/GSM246289.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
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GSM246290 | GPL570 |
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CMV rep6
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infected with recombinant adenovirus bearing no cDNA
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MDA-MB-231 cells
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CMV_rep6
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| Sample_geo_accession | GSM246290
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246290/suppl/GSM246290.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
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