Search results for the GEO ID: GSE9761  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM246267 | GPL570 |
|
CMV_rep1
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep1
|
| Sample_geo_accession | GSM246267
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246267/suppl/GSM246267.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246268 | GPL570 |
|
CMV_rep2
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep2
|
| Sample_geo_accession | GSM246268
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246268/suppl/GSM246268.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246269 | GPL570 |
|
CMV_rep3
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep3
|
| Sample_geo_accession | GSM246269
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246269/suppl/GSM246269.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246270 | GPL570 |
|
CMV_rep4
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep4
|
| Sample_geo_accession | GSM246270
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246270/suppl/GSM246270.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246271 | GPL570 |
|
CMV_rep5
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep5
|
| Sample_geo_accession | GSM246271
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246271/suppl/GSM246271.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246272 | GPL570 |
|
CMV_rep6
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep6
|
| Sample_geo_accession | GSM246272
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246272/suppl/GSM246272.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246273 | GPL570 |
|
Era_rep1
|
infected with recombinant adenovirus bearing cDNA for Eralpha
|
MDA-MB-231 cells
|
Era_rep1
|
| Sample_geo_accession | GSM246273
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246273/suppl/GSM246273.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246274 | GPL570 |
|
Era_rep2
|
infected with recombinant adenovirus bearing cDNA for Eralpha
|
MDA-MB-231 cells
|
Era_rep2
|
| Sample_geo_accession | GSM246274
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246274/suppl/GSM246274.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246275 | GPL570 |
|
Era_rep3
|
infected with recombinant adenovirus bearing cDNA for Eralpha
|
MDA-MB-231 cells
|
Era_rep3
|
| Sample_geo_accession | GSM246275
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246275/suppl/GSM246275.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246276 | GPL570 |
|
Era_rep4
|
infected with recombinant adenovirus bearing cDNA for Eralpha
|
MDA-MB-231 cells
|
Era_rep4
|
| Sample_geo_accession | GSM246276
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246276/suppl/GSM246276.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246277 | GPL570 |
|
Era_rep5
|
infected with recombinant adenovirus bearing cDNA for Eralpha
|
MDA-MB-231 cells
|
Era_rep5
|
| Sample_geo_accession | GSM246277
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246277/suppl/GSM246277.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246278 | GPL570 |
|
Era_rep6
|
infected with recombinant adenovirus bearing cDNA for Eralpha
|
MDA-MB-231 cells
|
Era_rep6
|
| Sample_geo_accession | GSM246278
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for Eralpha in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246278/suppl/GSM246278.CEL.gz
| | Sample_series_id | GSE9757
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246279 | GPL570 |
|
ERa3411 rep1
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep1
|
| Sample_geo_accession | GSM246279
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246279/suppl/GSM246279.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246280 | GPL570 |
|
ERa3411 rep2
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep2
|
| Sample_geo_accession | GSM246280
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246280/suppl/GSM246280.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246281 | GPL570 |
|
ERa3411 rep3
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep3
|
| Sample_geo_accession | GSM246281
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246281/suppl/GSM246281.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246282 | GPL570 |
|
ERa3411 rep4
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep4
|
| Sample_geo_accession | GSM246282
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246282/suppl/GSM246282.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246283 | GPL570 |
|
ERa3411 rep5
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep5
|
| Sample_geo_accession | GSM246283
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246283/suppl/GSM246283.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246284 | GPL570 |
|
ERa3411 rep6
|
infected with recombinant adenovirus bearing cDNA for ERalpha 203/204/211E (3411E)
|
MDA-MB-231 cells
|
ERa3411_rep6
|
| Sample_geo_accession | GSM246284
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246284/suppl/GSM246284.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246285 | GPL570 |
|
CMV rep1
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep1
|
| Sample_geo_accession | GSM246285
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246285/suppl/GSM246285.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246286 | GPL570 |
|
CMV rep2
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep2
|
| Sample_geo_accession | GSM246286
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246286/suppl/GSM246286.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246287 | GPL570 |
|
CMV rep3
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep3
|
| Sample_geo_accession | GSM246287
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246287/suppl/GSM246287.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246288 | GPL570 |
|
CMV rep4
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep4
|
| Sample_geo_accession | GSM246288
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246288/suppl/GSM246288.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246289 | GPL570 |
|
CMV rep5
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep5
|
| Sample_geo_accession | GSM246289
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246289/suppl/GSM246289.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246290 | GPL570 |
|
CMV rep6
|
infected with recombinant adenovirus bearing no cDNA
|
MDA-MB-231 cells
|
CMV_rep6
|
| Sample_geo_accession | GSM246290
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERalpha mutant defective in binding to ERE (3411E) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246290/suppl/GSM246290.CEL.gz
| | Sample_series_id | GSE9758
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54675
| | |
|
GSM246297 | GPL570 |
|
Erb rep 1
|
infected with recombinant adenovirus bearing cDNA for ERbeta; rep1
|
MDA-MB-231
|
Erb_rep1
|
| Sample_geo_accession | GSM246297
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246297/suppl/GSM246297.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246298 | GPL570 |
|
Erb rep 2
|
infected with recombinant adenovirus bearing cDNA for ERbeta; rep2
|
MDA-MB-231
|
Erb_rep2
|
| Sample_geo_accession | GSM246298
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246298/suppl/GSM246298.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246299 | GPL570 |
|
Erb rep 3
|
infected with recombinant adenovirus bearing cDNA for ERbeta; rep3
|
MDA-MB-231
|
Erb_rep3
|
| Sample_geo_accession | GSM246299
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246299/suppl/GSM246299.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246300 | GPL570 |
|
Erb rep 4
|
infected with recombinant adenovirus bearing cDNA for ERbeta; rep4
|
MDA-MB-231
|
Erb_rep4
|
| Sample_geo_accession | GSM246300
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246300/suppl/GSM246300.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246301 | GPL570 |
|
Erb rep 5
|
infected with recombinant adenovirus bearing cDNA for ERbeta; rep5
|
MDA-MB-231
|
Erb_rep5
|
| Sample_geo_accession | GSM246301
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246301/suppl/GSM246301.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246302 | GPL570 |
|
Erb rep 6
|
infected with recombinant adenovirus bearing cDNA for ERbeta; rep6
|
MDA-MB-231
|
Erb_rep6
|
| Sample_geo_accession | GSM246302
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246302/suppl/GSM246302.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246303 | GPL570 |
|
ErbDBD rep 1
|
infected with recombinant adenovirus bearing cDNA for ERbeta mutant defective in binding to ERE; rep1
|
MDA-MB-231
|
ErbDBD_rep1
|
| Sample_geo_accession | GSM246303
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246303/suppl/GSM246303.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246304 | GPL570 |
|
ErbDBD rep 2
|
infected with recombinant adenovirus bearing cDNA for ERbeta mutant defective in binding to ERE; rep2
|
MDA-MB-231
|
ErbDBD_rep2
|
| Sample_geo_accession | GSM246304
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246304/suppl/GSM246304.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246305 | GPL570 |
|
ErbDBD rep 3
|
infected with recombinant adenovirus bearing cDNA for ERbeta mutant defective in binding to ERE; rep3
|
MDA-MB-231
|
ErbDBD_rep3
|
| Sample_geo_accession | GSM246305
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246305/suppl/GSM246305.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246306 | GPL570 |
|
ErbDBD rep 4
|
infected with recombinant adenovirus bearing cDNA for ERbeta mutant defective in binding to ERE; rep4
|
MDA-MB-231
|
ErbDBD_rep4
|
| Sample_geo_accession | GSM246306
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246306/suppl/GSM246306.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246307 | GPL570 |
|
ErbDBD rep 5
|
infected with recombinant adenovirus bearing cDNA for ERbeta mutant defective in binding to ERE; rep5
|
MDA-MB-231
|
ErbDBD_rep5
|
| Sample_geo_accession | GSM246307
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246307/suppl/GSM246307.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
|
GSM246308 | GPL570 |
|
ErbDBD rep 6
|
infected with recombinant adenovirus bearing cDNA for ERbeta mutant defective in binding to ERE; rep6
|
MDA-MB-231
|
ErbDBD_rep6
|
| Sample_geo_accession | GSM246308
| | Sample_status | Public on Dec 04 2007
| | Sample_submission_date | Dec 03 2007
| | Sample_last_update_date | Aug 14 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Infected cells were treated with 17beta-estradiol for 6h
| | Sample_growth_protocol_ch1 | MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
| | Sample_hyb_protocol | 2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
| | Sample_scan_protocol | After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
| | Sample_data_processing | Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephen,,Welle
| | Sample_contact_email | stephen_welle@urmc.rochester.edu
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Avenue
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM246nnn/GSM246308/suppl/GSM246308.CEL.gz
| | Sample_series_id | GSE9759
| | Sample_series_id | GSE9761
| | Sample_data_row_count | 54613
| | |
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