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Search results for the GEO ID: GSE9773
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GSM246492
GPL96
EVT-1 First trimester placental organ explants grown on matrigel Dissected mesenchymal villous tissue of early pregnancy was arranged radially on 80 μl drops of growth-factor-reduced Matrigel™ (BD, Bedford, MA) in 24 well plates (SFM; DMEM:Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. Affymetrix
GSM246493
GPL96
EVT-2 First trimester placental organ explants grown on matrigel Dissected mesenchymal villous tissue of early pregnancy was arranged radially on 80 μl drops of growth-factor-reduced Matrigel™ (BD, Bedford, MA) in 24 well plates (SFM; DMEM:Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. Affymetrix
GSM246494
GPL96
EVT-3 First trimester placental organ explants grown on matrigel Dissected mesenchymal villous tissue of early pregnancy was arranged radially on 80 μl drops of growth-factor-reduced Matrigel™ (BD, Bedford, MA) in 24 well plates (SFM; DMEM:Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. Affymetrix
GSM246495
GPL96
CTB-1 First trimester cytotrophoblast cells grown in culture for 24 hours First trimester placental villous material was digested with a 0.125% (v/v) trypsin solution (GIBCO, Life Technologies, Paisley, UK). The released cells were loaded on top of a Percoll (Pharmacia, Uppsala, Sweden) gradient ranging from 70 to 10% (v/v). After centrifugation, the band containing the trophoblasts was collected and sequentially purified using immunomagnetic beads (Dynabeads M-450, Dynal) conjugated to CD45 RB leukocyte common antigen (Dako, CA) and to fibroblast specific antibody A 502 (Dianova, Hamburg). Trophoblasts were cultured for 24 h in DMEM (GIBCO) with 10% FCS, 100 mg/ml streptomycin and 100 IU/ml penicillin (GIBCO). Affymetrix
GSM246497
GPL96
CTB-2 First trimester cytotrophoblast cells grown in culture for 24 hours. First trimester placental villous material was digested with a 0.125% (v/v) trypsin solution (GIBCO, Life Technologies, Paisley, UK). The released cells were loaded on top of a Percoll (Pharmacia, Uppsala, Sweden) gradient ranging from 70 to 10% (v/v). After centrifugation, the band containing the trophoblasts was collected and sequentially purified using immunomagnetic beads (Dynabeads M-450, Dynal) conjugated to CD45 RB leukocyte common antigen (Dako, CA) and to fibroblast specific antibody A 502 (Dianova, Hamburg). Trophoblasts were cultured for 24 h in DMEM (GIBCO) with 10% FCS, 100 mg/ml streptomycin and 100 IU/ml penicillin (GIBCO). Affymetrix
GSM246499
GPL96
CTB-3 First trimester cytotrophoblast cells grown in culture for 24 hours. First trimester placental villous material was digested with a 0.125% (v/v) trypsin solution (GIBCO, Life Technologies, Paisley, UK). The released cells were loaded on top of a Percoll (Pharmacia, Uppsala, Sweden) gradient ranging from 70 to 10% (v/v). After centrifugation, the band containing the trophoblasts was collected and sequentially purified using immunomagnetic beads (Dynabeads M-450, Dynal) conjugated to CD45 RB leukocyte common antigen (Dako, CA) and to fibroblast specific antibody A 502 (Dianova, Hamburg). Trophoblasts were cultured for 24 h in DMEM (GIBCO) with 10% FCS, 100 mg/ml streptomycin and 100 IU/ml penicillin (GIBCO). Affymetrix
GSM307639
GPL96
EVT-4 First trimester placental organ explants grown on matrigel Dissected mesenchymal villous tissue of early pregnancy was arranged radially on 80 μl drops of growth-factor-reduced Matrigel™ (BD, Bedford, MA) in 24 well plates (SFM; DMEM:Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. Affymetrix
GSM307640
GPL96
EVT-5 First trimester placental organ explants grown on matrigel Dissected mesenchymal villous tissue of early pregnancy was arranged radially on 80 μl drops of growth-factor-reduced Matrigel™ (BD, Bedford, MA) in 24 well plates (SFM; DMEM:Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. Affymetrix
GSM307641
GPL96
EVT-6 First trimester placental organ explants grown on matrigel Dissected mesenchymal villous tissue of early pregnancy was arranged radially on 80 μl drops of growth-factor-reduced Matrigel™ (BD, Bedford, MA) in 24 well plates (SFM; DMEM:Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation Affymetrix
GSM307642
GPL96
CTB-4 First trimester cytotrophoblast cells grown in culture for 24 hours First trimester placental villous material was digested with a 0.125% (v/v) trypsin solution (GIBCO, Life Technologies, Paisley, UK). The released cells were loaded on top of a Percoll (Pharmacia, Uppsala, Sweden) gradient ranging from 70 to 10% (v/v). After centrifugation, the band containing the trophoblasts was collected and sequentially purified using immunomagnetic beads (Dynabeads M-450, Dynal) conjugated to CD45 RB leukocyte common antigen (Dako, CA) and to fibroblast specific antibody A 502 (Dianova, Hamburg). Trophoblasts were cultured for 24 h in DMEM (GIBCO) with 10% FCS, 100 mg/ml streptomycin and 100 IU/ml penicillin (GIBCO). Affymetrix
GSM307643
GPL96
CTB-5 First trimester cytotrophoblast cells grown in culture for 24 hours First trimester placental villous material was digested with a 0.125% (v/v) trypsin solution (GIBCO, Life Technologies, Paisley, UK). The released cells were loaded on top of a Percoll (Pharmacia, Uppsala, Sweden) gradient ranging from 70 to 10% (v/v). After centrifugation, the band containing the trophoblasts was collected and sequentially purified using immunomagnetic beads (Dynabeads M-450, Dynal) conjugated to CD45 RB leukocyte common antigen (Dako, CA) and to fibroblast specific antibody A 502 (Dianova, Hamburg). Trophoblasts were cultured for 24 h in DMEM (GIBCO) with 10% FCS, 100 mg/ml streptomycin and 100 IU/ml penicillin (GIBCO). Affymetrix
 
 
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