Search results for the GEO ID: GSE9894 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM250019 | GPL570 |
|
BM-MSC culture rep 1
|
bone marrow MSC in culture from normal donor 1
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 67 years
treatment: none
|
Gene expression data of human bone marrow derived mesenchymal stem cells.
BM-MSC of a male 67-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage.
|
Sample_geo_accession | GSM250019
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | None.
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250019/suppl/GSM250019.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250019/suppl/GSM250019.EXP.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE9894
| Sample_series_id | GSE18043
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250020 | GPL570 |
|
BM-MSC culture rep 2
|
bone marrow MSC in culture from normal donor 2
|
cell type: bone marrow-derived mesenchymal stem cells
gender: male
age: 72 years
treatment: none
|
Gene expression data of human bone marrow derived mesenchymal stem cells.
BM-MSC of a male 72-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage.
|
Sample_geo_accession | GSM250020
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | None.
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250020/suppl/GSM250020.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250020/suppl/GSM250020.EXP.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE9894
| Sample_series_id | GSE18043
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250021 | GPL570 |
|
BM-MSC culture rep 3
|
bone marrow MSC in culture from normal donor 3
|
cell type: bone marrow-derived mesenchymal stem cells
gender: female
age: 74 years
treatment: none
|
Gene expression data of human bone marrow derived mesenchymal stem cells.
BM-MSC of a female 74-years old cultured in alpha-MEM + 10% FCS + 1 ng/mL FGF2 for 1 passage.
|
Sample_geo_accession | GSM250021
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | None.
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250021/suppl/GSM250021.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250021/suppl/GSM250021.EXP.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE9894
| Sample_series_id | GSE18043
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250022 | GPL570 |
|
CD235a+ sorted rep 1
|
CD235a+ cells sorted from bone marrow aspirates of normal donor 1
|
cell type: erythropoietic cells sorted by CD235a labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow erythroblasts
|
Sample_geo_accession | GSM250022
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250022/suppl/GSM250022.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250022/suppl/GSM250022.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250023 | GPL570 |
|
CD235a+ sorted rep 2
|
CD235a+ cells sorted from bone marrow aspirates of normal donor 2
|
cell type: erythropoietic cells sorted by CD235a labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow erythroblasts
|
Sample_geo_accession | GSM250023
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250023/suppl/GSM250023.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250023/suppl/GSM250023.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250024 | GPL570 |
|
CD235a+ sorted rep 3
|
CD235a+ cells sorted from bone marrow aspirates of normal donor 3
|
cell type: erythropoietic cells sorted by CD235a labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow erythroblasts
|
Sample_geo_accession | GSM250024
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250024/suppl/GSM250024.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250024/suppl/GSM250024.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250025 | GPL570 |
|
CD11b+ sorted rep 1
|
CD11b+ cells sorted from bone marrow aspirates of normal donor 1
|
cell type: granulopoietic cells sorted by CD11b labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow myeloid cells (granulomonocytic series )
|
Sample_geo_accession | GSM250025
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250025/suppl/GSM250025.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250025/suppl/GSM250025.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250026 | GPL570 |
|
CD11b+ sorted rep 2
|
CD11b+ cells sorted from bone marrow aspirates of normal donor 2
|
cell type: granulopoietic cells sorted by CD11b labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow myeloid cells (granulomonocytic series )
|
Sample_geo_accession | GSM250026
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250026/suppl/GSM250026.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250026/suppl/GSM250026.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250027 | GPL570 |
|
CD11b+ sorted rep 3
|
CD11b+ cells sorted from bone marrow aspirates of normal donor 3
|
cell type: granulopoietic cells sorted by CD11b labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow myeloid cells (granulomonocytic series )
|
Sample_geo_accession | GSM250027
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250027/suppl/GSM250027.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250027/suppl/GSM250027.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250028 | GPL570 |
|
CD45+ sorted rep 1
|
CD45+ cells sorted from bone marrow aspirates of normal donor 1
|
cell type: hematopoietic cells sorted by CD45 labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow hematopoietic cells (mostly granulomonocytic series and lymphocytes)
|
Sample_geo_accession | GSM250028
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250028/suppl/GSM250028.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250028/suppl/GSM250028.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250029 | GPL570 |
|
CD45+ sorted rep 2
|
CD45+ cells sorted from bone marrow aspirates of normal donor 2
|
cell type: hematopoietic cells sorted by CD45 labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow hematopoietic cells (mostly granulomonocytic series and lymphocytes)
|
Sample_geo_accession | GSM250029
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250029/suppl/GSM250029.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250029/suppl/GSM250029.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
GSM250030 | GPL570 |
|
CD45+ sorted rep 3
|
CD45+ cells sorted from bone marrow aspirates of normal donor 3
|
cell type: hematopoietic cells sorted by CD45 labeling of BM cells immediately after BM aspiration
|
gene expression data of human bone marrow hematopoietic cells (mostly granulomonocytic series and lymphocytes)
|
Sample_geo_accession | GSM250030
| Sample_status | Public on Dec 15 2007
| Sample_submission_date | Dec 14 2007
| Sample_last_update_date | Oct 08 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | CD235a+, CD45+ and CD11b+ hematopoietic fractions were isolated from BM samples using MACS (Miltenyi).
| Sample_growth_protocol_ch1 | Human BM cells obtained from iliac crest aspirates were amplified by cultivation in alpha-MEM with 10 % fetal calf serum (FCS), 1 ng/mL fibroblast growth factor 2 (FGF2), 20 µmol/L L-glutamine and 100 units/mL penicillin G
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was collected using the RNeasy mini kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2µg of total RNA was amplified and labeled using the GeneChip® one-cycle target labeling and control reagents (Affymetrix)
| Sample_hyb_protocol | 50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing = Data were processed with GCOS 1.4 (TGT | 150) and subsequently imported and further analyzed in the online database (www.bioretis.de).
| Sample_platform_id | GPL570
| Sample_contact_name | Thomas,,Häupl
| Sample_contact_email | thomas.haeupl@charite.de
| Sample_contact_phone | +49(0)30 450513293
| Sample_contact_fax | +49(0)30 450513957
| Sample_contact_department | Rheumatology
| Sample_contact_institute | Charité
| Sample_contact_address | Tucholskystr. 2
| Sample_contact_city | Berlin
| Sample_contact_zip/postal_code | 10117
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250030/suppl/GSM250030.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250030/suppl/GSM250030.EXP.gz
| Sample_series_id | GSE9894
| Sample_series_id | GSE24598
| Sample_data_row_count | 54675
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|