Search results for the GEO ID: GSE9918 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM250998 | GPL1355 |
|
NAIVE A
|
naive adult rat retina
|
naive
|
RNA from naive adult rat retina rep 1
|
Sample_geo_accession | GSM250998
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250998/suppl/GSM250998.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM250999 | GPL1355 |
|
NAIVE B
|
naive adult rat retina
|
naive
|
RNA from naive adult rat retina rep 2
|
Sample_geo_accession | GSM250999
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM250nnn/GSM250999/suppl/GSM250999.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251000 | GPL1355 |
|
NAIVE C
|
naive adult rat retina
|
naive
|
RNA from naive adult rat retina rep 3
|
Sample_geo_accession | GSM251000
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251000/suppl/GSM251000.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251001 | GPL1355 |
|
NAIVE D
|
naive adult rat retina
|
naive
|
RNA from naive adult rat retina rep 4
|
Sample_geo_accession | GSM251001
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251001/suppl/GSM251001.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251002 | GPL1355 |
|
NAIVE E
|
naive adult rat retina
|
naive
|
RNA from naive adult rat retina rep 5
|
Sample_geo_accession | GSM251002
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251002/suppl/GSM251002.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251003 | GPL1355 |
|
AXO 12H A
|
retinas 12h post IONT
|
time and injury 12h post IONT
|
RNA from retinas 12h post IONT rep 1
|
Sample_geo_accession | GSM251003
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251003/suppl/GSM251003.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251004 | GPL1355 |
|
AXO 12H B
|
retinas 12h post IONT
|
time and injury 12h post IONT
|
RNA from retinas 12h post IONT rep 2
|
Sample_geo_accession | GSM251004
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251004/suppl/GSM251004.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251005 | GPL1355 |
|
AXO 12H C
|
retinas 12h post IONT
|
time and injury 12h post IONT
|
RNA from retinas 12h post IONT rep 3
|
Sample_geo_accession | GSM251005
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251005/suppl/GSM251005.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251006 | GPL1355 |
|
AXO 24H B
|
retinas 24h post IONT
|
time and injury 24h post IONT
|
RNA from retinas 24h post IONT rep 1
|
Sample_geo_accession | GSM251006
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251006/suppl/GSM251006.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251007 | GPL1355 |
|
AXO 24H A
|
retinas 24h post IONT
|
time and injury 24h post IONT
|
RNA from retinas 24h post IONT rep 2
|
Sample_geo_accession | GSM251007
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251007/suppl/GSM251007.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251008 | GPL1355 |
|
AXO 48H A
|
retinas 48h post IONT
|
time and injury 48h post IONT
|
RNA from retinas 48h post IONT rep 1
|
Sample_geo_accession | GSM251008
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251008/suppl/GSM251008.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251009 | GPL1355 |
|
AXO 48H B
|
retinas 48h post IONT
|
time and injury 48h post IONT
|
RNA from retinas 48h post IONT rep 2
|
Sample_geo_accession | GSM251009
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251009/suppl/GSM251009.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251010 | GPL1355 |
|
AXO 48H C
|
retinas 48h post IONT
|
time and injury 48h post IONT
|
RNA from retinas 48h post IONT rep 3
|
Sample_geo_accession | GSM251010
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251010/suppl/GSM251010.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251011 | GPL1355 |
|
AXO 3D A
|
retinas 3d post IONT
|
time and injury 3d post IONT
|
RNA from retinas 3d post IONT rep 1
|
Sample_geo_accession | GSM251011
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251011/suppl/GSM251011.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251012 | GPL1355 |
|
AXO 3D B
|
retinas 3d post IONT
|
time and injury 3d post IONT
|
RNA from retinas 3d post IONT rep 2
|
Sample_geo_accession | GSM251012
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251012/suppl/GSM251012.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251013 | GPL1355 |
|
AXO 7D A
|
retinas 7d post IONT
|
time and injury 7d post IONT
|
RNA from retinas 7d post IONT rep 1
|
Sample_geo_accession | GSM251013
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251013/suppl/GSM251013.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251014 | GPL1355 |
|
AXO 7D B
|
retinas 7d post IONT
|
time and injury 7d post IONT
|
RNA from retinas 7d post IONT rep 2
|
Sample_geo_accession | GSM251014
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251014/suppl/GSM251014.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251015 | GPL1355 |
|
AXO 7D C
|
retinas 7d post IONT
|
time and injury 7d post IONT
|
RNA from retinas 7d post IONT rep 3
|
Sample_geo_accession | GSM251015
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251015/suppl/GSM251015.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251016 | GPL1355 |
|
AXO 15D A
|
retinas 15d post IONT
|
time and injury 15d post IONT
|
RNA from retinas 15d post IONT rep 1
|
Sample_geo_accession | GSM251016
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251016/suppl/GSM251016.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251017 | GPL1355 |
|
AXO 15D B
|
retinas 15d post IONT
|
time and injury 15d post IONT
|
RNA from retinas 15d post IONT rep 2
|
Sample_geo_accession | GSM251017
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251017/suppl/GSM251017.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251018 | GPL1355 |
|
CRUSH 12H A
|
retinas 12h post IONC
|
time and injury 12h post IONC
|
RNA from retinas 12h post IONC rep 1
|
Sample_geo_accession | GSM251018
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251018/suppl/GSM251018.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251019 | GPL1355 |
|
CRUSH 12H B
|
retinas 12h post IONC
|
time and injury 12h post IONC
|
RNA from retinas 12h post IONC rep 2
|
Sample_geo_accession | GSM251019
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251019/suppl/GSM251019.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251020 | GPL1355 |
|
CRUSH 12H C
|
retinas 12h post IONC
|
time and injury 12h post IONC
|
RNA from retinas 12h post IONC rep 3
|
Sample_geo_accession | GSM251020
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251020/suppl/GSM251020.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251021 | GPL1355 |
|
CRUSH 24H A
|
retinas 24h post IONC
|
time and injury 24h post IONC
|
RNA from retinas 24h post IONC rep 1
|
Sample_geo_accession | GSM251021
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251021/suppl/GSM251021.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251022 | GPL1355 |
|
CRUSH 24H B
|
retinas 24h post IONC
|
time and injury 24h post IONC
|
RNA from retinas 24h post IONC rep 2
|
Sample_geo_accession | GSM251022
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251022/suppl/GSM251022.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251023 | GPL1355 |
|
CRUSH 24H C
|
retinas 24h post IONC
|
time and injury 24h post IONC
|
RNA from retinas 24h post IONC rep 3
|
Sample_geo_accession | GSM251023
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251023/suppl/GSM251023.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251024 | GPL1355 |
|
CRUSH 48H A
|
retinas 48h post IONC
|
time and injury 48h post IONC
|
RNA from retinas 48h post IONC rep 1
|
Sample_geo_accession | GSM251024
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251024/suppl/GSM251024.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251025 | GPL1355 |
|
CRUSH 48H B
|
retinas 48h post IONC
|
time and injury 48h post IONC
|
RNA from retinas 48h post IONC rep 2
|
Sample_geo_accession | GSM251025
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251025/suppl/GSM251025.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251026 | GPL1355 |
|
CRUSH 48H C
|
retinas 48h post IONC
|
time and injury 48h post IONC
|
RNA from retinas 48h post IONC rep 3
|
Sample_geo_accession | GSM251026
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251026/suppl/GSM251026.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251027 | GPL1355 |
|
CRUSH 3D A
|
retinas 3d post IONC
|
time and injury 3d post IONC
|
RNA from retinas 3d post IONC rep 1
|
Sample_geo_accession | GSM251027
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251027/suppl/GSM251027.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251028 | GPL1355 |
|
CRUSH 3D B
|
retinas 3d post IONC
|
time and injury 3d post IONC
|
RNA from retinas 3d post IONC rep 2
|
Sample_geo_accession | GSM251028
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251028/suppl/GSM251028.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251029 | GPL1355 |
|
CRUSH 3D C
|
retinas 3d post IONC
|
time and injury 3d post IONC
|
RNA from retinas 3d post IONC rep 3
|
Sample_geo_accession | GSM251029
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251029/suppl/GSM251029.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251030 | GPL1355 |
|
CRUSH 7D A
|
retinas 7d post IONC
|
time and injury 7d post IONC
|
RNA from retinas 7d post IONC rep 1
|
Sample_geo_accession | GSM251030
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251030/suppl/GSM251030.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251031 | GPL1355 |
|
CRUSH 7D B
|
retinas 7d post IONC
|
time and injury 7d post IONC
|
RNA from retinas 7d post IONC rep 2
|
Sample_geo_accession | GSM251031
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251031/suppl/GSM251031.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
GSM251032 | GPL1355 |
|
CRUSH 7D C
|
retinas 7d post IONC
|
time and injury 7d post IONC
|
RNA from retinas 7d post IONC rep 3
|
Sample_geo_accession | GSM251032
| Sample_status | Public on Jun 13 2008
| Sample_submission_date | Dec 17 2007
| Sample_last_update_date | Jun 13 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | naive rats were not treated. IONT rats underwent introorbital nerve transection, IONC rats intraorbital nerve crush
| Sample_growth_protocol_ch1 | Rats were kept in standard housing condintions
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and was following by a further cleansing step through RNAeasy mini kit columns (Quiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on Rat norvergicous Affymetrix GeneChip Rat Genome 230 2.0 Array
| Sample_scan_protocol | Gene Chip Operating software, (GCOS 1.3 Afffymetrix, Santa Clara CA)
| Sample_data_processing | Data were processing using Bioconductor software, normalization was done with the GCRMA package. IONT and IONC samples at each time point were compared to naive ones. Significantly regulated sequences were extracted using the limma package (Bvalue>0, pvalueFDR<0,01) and the maSigPro package for time course series (pvalueFDR<0,01, Rsquared treshold=0,6).Log2 output values of regulated sequences were transformed to their net (fold change) values. Control level of expresion was set to 1, above 1 are up-regulated sequences and below 1 to 0 downregulated (when a RNA is not present its value is not negative but 0).
| Sample_platform_id | GPL1355
| Sample_contact_name | MARTA,,AGUDO
| Sample_contact_email | martabar@um.es
| Sample_contact_department | OFTALMOLOGY
| Sample_contact_institute | MURCIA UNIVERSITY
| Sample_contact_address | FACULTAD MEDICINA, CAMPUS ESPINARDO
| Sample_contact_city | MURCIA
| Sample_contact_zip/postal_code | 30100
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM251nnn/GSM251032/suppl/GSM251032.CEL.gz
| Sample_series_id | GSE9918
| Sample_data_row_count | 31099
| |
|
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