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Search results for the GEO ID: GSE9927
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GPL ID
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Title
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Description
Characteristics
GSM251101
GPL570
Normal Control C-10a Normal Control C-10a CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251105
GPL570
Normal Control C-1a Normal Control CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251110
GPL570
Normal Control C-2a Normal Control C-2a CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251111
GPL570
Normal Control C-3a Control C-3a CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251114
GPL570
Normal Control C-4a Normal Control C-4a CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251126
GPL570
Normal Control C-5a Normal Control C-5a CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251129
GPL570
Normal Control C-6a Normal Control C-6a CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251192
GPL570
Normal Control C-7a Normal Control C-7a CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251194
GPL570
Normal Control C-8a Normal Control C-8a CD4+ T-cells from normal HIV- donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251195
GPL570
HIV+ Individual HIV-10a HIV+ Individual HIV-10a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251196
GPL570
HIV+ Individual HIV-11a HIV+ Individual HIV-11a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251197
GPL570
HIV+ Individual HIV-1a HIV+ Individual HIV-1a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251198
GPL570
HIV+ Individual HIV-2a HIV+ Individual HIV-2a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251199
GPL570
HIV+ Individual HIV-3a HIV+ Individual HIV-3a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251200
GPL570
HIV+ Individual HIV-4a HIV+ Individual HIV-4a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251207
GPL570
HIV+ Individual HIV-5a HIV+ Individual HIV-5a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251208
GPL570
HIV+ Individual HIV-6a HIV+ Individual HIV-6a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251209
GPL570
HIV+ Individual HIV-7a HIV+ Individual HIV-7a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251210
GPL570
HIV+ Individual HIV-8a HIV+ Individual HIV-8a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
GSM251211
GPL570
HIV+ Individual HIV-9a HIV+ Individual HIV-9a CD4+ T-cells from HIV+ donor Activated CD4+ T cells were similarly isolated by positive selection from CD4+ T cells using CD25 microbeads (Miltenyi Biotec, Auburn, CA). In order to ensure isolation of CD25lo cells, the manufacturer’s protocol was altered such that 2 μL of CD25 microbeads and 8 μL of wash media (PBS pH 7.2-7.4, 2% newborn calf serum, 0.1% glucose, 1% pen/strep, 12mM HEPES pH 7.2) were used per million CD4+ T cells. Purity of isolated CD25+ cells was almost always greater than 95%.
 
 
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